Citation: Guangjin Li, Jiali Wang, Dongyang Li, Shenghua Liu, Jun Yin, Zhibing Lai, Guangfu Yang. A Hg(Ⅱ)-specific probe for imaging application in living systems and quantitative analysis in environmental/food samples[J]. Chinese Chemical Letters, ;2021, 32(4): 1527-1531. doi: 10.1016/j.cclet.2020.09.040 shu

A Hg(Ⅱ)-specific probe for imaging application in living systems and quantitative analysis in environmental/food samples

Guangjin Li ^a ,
Jiali Wang ^b ,
Dongyang Li ^a ,
Shenghua Liu ^a ,
Jun Yin ^{a, *,} ,
Zhibing Lai ^{b, *,} ,
Guangfu Yang ^{a, *,}

a.
Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, Hubei International Scientific and Technological Cooperation Base of Pesticide and Green Synthesis, International Joint Research Center for Intelligent Biosensing Technology and Health, College of Chemistry, Central China Normal University, Wuhan 430079, China
b.
National Key Laboratory of Crop Genetics Improvement, Huazhong Agricultural University, Wuhan 430070, China

yinj@mail.ccnu.edu.cn

zhibing@mail.hzau.edu.cn

gfyang@mail.ccnu.edu.cn

Received Date: 15 August 2020
Revised Date: 22 September 2020
Accepted Date: 23 September 2020
Available Online: 24 September 2020

Figures(4)

Mercury ions are highly toxic and can accumulate along food chains in water, soil, crops and animals. Effective detection of mercury ions in various media is of great significance for maintaining the ecological environment and protecting people's health. In this work, a mercury ions specific fluorescent probe was developed by a simple one-step reaction of commercial substrates of 4-chloro-7-nitro-2, 1, 3-benzoxadiazole and 1-(2-aminoethyl)-4-methylpiperazine. Investigation on sensing behavior showed that this probe had high sensitivity and selectivity towards mercury ions. Furthermore, this probe could be used as a tool to track the level of mercury ions in living system. In living cells, the probe with green emission emitted a bright red fluorescence when it was bound to mercury ions. In Arabidopsis thaliana, similar red emission could be detected from the root tip and stalk when A. thaliana was grown in culture medium containing mercury ions. The imaging in zebrafish showed that mercury ions were mainly concentrated in the stomach and head of zebrafish. Especially, this probe could be applied in quantitative analysis of mercury ions in tap water, green tea, sea shrimp and soil. This work provided a practical tool for the detection of mercury ions in living systems and quantitative analysis in real samples.
- Fluorescent probe,
- Cell imaging,
- Plant imaging,
- Zebrafish imaging,
- Quantitative analysis
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