Citation: Xiaojie Ren, Lide Liao, Zhaoguang Yang, Haipu Li, Xi Li, Yangang Wang, Yong Ye, Xiangzhi Song. Rational design of a bifunctional fluorescent probe for distinguishing Hcy/Cys from GSH with ideal properties[J]. Chinese Chemical Letters, ;2021, 32(3): 1061-1065. doi: 10.1016/j.cclet.2020.09.024 shu

Rational design of a bifunctional fluorescent probe for distinguishing Hcy/Cys from GSH with ideal properties

Xiaojie Ren ^{a, 1} ,
Lide Liao ^{a, 1} ,
Zhaoguang Yang ^{a, b} ,
Haipu Li ^{a, b} ,
Xi Li ^c ,
Yangang Wang ^{c, **,} ,
Yong Ye ^d ,
Xiangzhi Song ^{a, b, *,}

a.
College of Chemistry & Chemical Engineering, Central South University, Changsha 410083, China
b.
Key Laboratory of Hunan Province for Water Environment and Agriculture Product Safety, Changsha 410083, China
c.
College of Biological Chemical Science and Engineering, Jiaxing University, Jiaxing 314001, China
d.
College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, China

^**

Received Date: 4 July 2020
Revised Date: 14 September 2020
Accepted Date: 15 September 2020
Available Online: 16 September 2020

Figures(9)

By pairing two fluorophores according to their optical properties such as absorption spectral overlap and absorptivity, fluorescent quantum yield and emission spectral separation, a bifunctional fluorescent probe, TQBF-NBD, was rationally designed and synthesized to discriminatively sense Hcy/Cys and GSH with good selectivity and sensitivity. It is noted that this probe could work under a single-wavelength excitation and displayed a mega-large Stokes shift. TQBF-NBD reacted with Hcy/Cys to give a mixed green-red fluorescence and displayed a red fluorescence upon the treatment with GSH. Distinguishable imaging of intracellular Hcy/Cys from GSH with the help of TQBF-NBD was realized in living cells and zebrafish.
- Borate complex,
- Red-emitting,
- Mega-large stokes shift,
- Fluorescent probe
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沈阳化工大学材料科学与工程学院沈阳 110142