Citation: Hua Chai, Peng Miao. Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures[J]. Chinese Chemical Letters, ;2021, 32(2): 783-786. doi: 10.1016/j.cclet.2020.06.030 shu

Ultrasensitive assay of ctDNA based on DNA triangular prism and three-way junction nanostructures

Hua Chai ^{a, b} ,
Peng Miao ^{a, *,}

a.
Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou 215163, China
b.
Jinan Guokeyigong Science and Technology Development Co., Ltd., Ji'nan 250103, China

miaopeng@sibet.ac.cn

Received Date: 18 May 2020
Revised Date: 7 June 2020
Accepted Date: 20 June 2020
Available Online: 22 June 2020

Figures(4)

Circulating tumor DNA (ctDNA) refers to a class of acellular nucleic acids carrying genetic features of primary tumor, which can be regarded as a promising noninvasive biomarker for cancer diagnosis. The development of ctDNA assay is an important component of liquid biopsy. In this study, we have fabricated a novel electrochemical strategy for ultrasensitive detection of ctDNA combining the merits of strand displacement amplification and DNA nanostructures. Stable DNA triangular prism is firstly self-assembled and modified on the electrode surface. After target initiated strand displacement polymerization reaction, the generated DNA product helps the formation of three-way junction nanostructure on triangular prism, which localizes electrochemical species. By carefully investigating the electrochemical responses, the limit of detection (LOD) for ctDNA assay as low as 48 amol/L is achieved. This proposed electrochemical biosensor shows great potential for clinical applications.
- Circulating tumor DNA,
- Biosensor,
- DNA triangular prism,
- DNA three-way junction,
- Strand displacement polymerization
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