Citation: Zishan Feng, Wei Zhang, Longjie Li, Bocheng Tu, Weicong Ye, Xiaofeng Tang, Hongbo Wang, Xianjin Xiao, Tongbo Wu. A cost-effective detection of low-abundance mutation with DNA three-way junction structure and lambda exonuclease[J]. Chinese Chemical Letters, ;2021, 32(2): 779-782. doi: 10.1016/j.cclet.2020.06.009 shu

A cost-effective detection of low-abundance mutation with DNA three-way junction structure and lambda exonuclease

Zishan Feng ^a ,
Wei Zhang ^{b, c} ,
Longjie Li ^a ,
Bocheng Tu ^a ,
Weicong Ye ^a ,
Xiaofeng Tang ^b ,
Hongbo Wang ^c ,
Xianjin Xiao ^{a, b, c, *,} ,
Tongbo Wu ^{a, b, *,}

a.
School of Pharmacy, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
b.
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
c.
Department of Obstetrics and Gynaecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China

xiaoxianjin@hust.edu.cn

wutongbo@hust.edu.cn

Received Date: 10 May 2020
Revised Date: 28 May 2020
Accepted Date: 5 June 2020
Available Online: 17 June 2020

Figures(4)

We presented a low-abundance mutation detection method with lambda exonuclease and DNA three-way junction structure. The assistant strand in the DNA three-way junction structure could regulate the reaction system from the kinetics and thermodynamics aspects. The optimization of the assistant strand helps to improve the selectivity of the mutant-type DNA to the wild-type DNA about 35 times. Moreover, the cost of the optimization process could be saved by about 90%. The method was applied to the detection of a human ovarian cancer-related gene mutation BRCA1 (rs1799949, c.2082C>T). The limit of detection to the mutation abundance in the DNA three-way junction structure system (0.2%) was one order lower compared with that in the double-stranded DNA structure system (2%). The mutation abundance in different standard samples was quantitively measured, and the results were consistent with the initial abundance in the standard samples.
- Gene mutation,
- Cancer,
- Low abundance,
- DNA three-way junction structure,
- Lambda exonuclease
1. [1]
  K. Cibulskis, M.S. Lawrence, S.L. Carter, et al., Nat. Biotechnol. 31 (2013) 213-219. doi: 10.1038/nbt.2514
2. [2]
  J.C.M. Wan, C. Massie, J. Garcia-Corbacho, et al., Nat. Rev. Cancer 17 (2017) 223-238. doi: 10.1038/nrc.2017.7
3. [3]
  A. Letai, Nat. Med. 23 (2017) 1028-1035. doi: 10.1038/nm.4389
4. [4]
  G. De Rubis, S.R. Krishnan, M. Bebawy, Trends Pharmacol. Sci. 40 (2019) 172-186. doi: 10.1016/j.tips.2019.01.006
5. [5]
  V. Cucchiara, M.R. Cooperberg, M. Dall' Era, et al., Eur. Urol. 73 (2018) 572-582. doi: 10.1016/j.eururo.2017.10.036
6. [6]
  J.J. Zhang, X.L. Pan, C.E. Bell, Biochemistry 53 (2014) 7415-7425. doi: 10.1021/bi501155q
7. [7]
  J. Li, L.L. Wang, H. Mamon, et al., Nat. Med. 14 (2008) 579-584. doi: 10.1038/nm1708
8. [8]
  H. Kimura, K. Kasahara, M. Kawaishi, et al., Clin. Cancer Res. 12 (2006) 3915-3921. doi: 10.1158/1078-0432.CCR-05-2324
9. [9]
  T. Forshew, M. Murtaza, C. Parkinson, et al., Sci. Transl. Med. 4 (2012) 136ra68.
10. [10]
  A.M. Newman, S.V. Bratman, J. To, et al., Nat. Med. 20 (2014) 552-558.
11. [11]
  K. Knez, D. Spasic, K.P.F. Janssen, J. Lammertyn, Analyst 139 (2014) 353-370. doi: 10.1039/C3AN01436C
12. [12]
  Y.V. Gerasimova, D.M. Kolpashchikov, Chem. Soc. Rev. 43 (2014) 6405-6438. doi: 10.1039/C4CS00083H
13. [13]
  D.M. Kolpashchikov, J. Am. Chem. Soc. 128 (2006) 10625-10628. doi: 10.1021/ja0628093
14. [14]
  S.X. Chen, D.Y. Zhang, G. Seelig, Nat. Chem. 5 (2013) 782-789. doi: 10.1038/nchem.1713
15. [15]
  M. Bai, F. Chen, X. Cao, et al., Angew. Chem. Int. Ed. 59 (2020) 13267-13272. doi: 10.1002/anie.202001598
16. [16]
  X.J. Xiao, T.B. Wu, L. Xu, W. Chen, M.P. Zhao, Nucleic Acids Res. 45 (2017) e90. doi: 10.1093/nar/gkx117
17. [17]
  L. Zhou, Y. Wang, C. Yang, et al., Biosens. Bioelectron. 126 (2019) 657-663. doi: 10.1016/j.bios.2018.11.028
18. [18]
  J.S. Wang, D.Y. Zhang, Nat. Chem. 7 (2015) 545-553. doi: 10.1038/nchem.2266
19. [19]
  X.J. Xiao, C. Song, C. Zhang, X. Su, M.P. Zhao, Chem. Commun. 48 (2012) 1964-1966. doi: 10.1039/C1CC15360A
20. [20]
  T.B. Wu, X.J. Xiao, F.D. Gu, M.P. Zhao, Chem. Commun. 51 (2015) 17402-17405. doi: 10.1039/C5CC05749C
21. [21]
  T.B. Wu, X.J. Xiao, Z. Zhang, M.P. Zhao, Chem. Sci. 6 (2015) 1206-1211. doi: 10.1039/C4SC03375B
22. [22]
  J.J. Xu, L.J. Li, N. Chen, et al., Chem. Commun. 53 (2017) 9422-9425. doi: 10.1039/C7CC04816E
23. [23]
  F.M. Shen, H. Qian, Y.L. Cheng, et al., Talanta 206 (2020) 120216. doi: 10.1016/j.talanta.2019.120216
24. [24]
  X. Chen, N. Liu, L.Q. Liu, et al., Nat. Commun. 10 (2019) 4675. doi: 10.1038/s41467-019-12593-9
25. [25]
  T.B. Wu, Y.F. Yang, W. Chen, et al., Nucleic Acids Res. 46 (2018) 3119-3129. doi: 10.1093/nar/gky154
26. [26]
  T.B. Wu, W. Chen, Z.Y. Yang, et al., Nucleic Acids Res. 46 (2018) e24. doi: 10.1093/nar/gkx1218
27. [27]
  D.Y. Zhang, S.X. Chen, P. Yin, Nat. Chem. 4 (2012) 208-214. doi: 10.1038/nchem.1246
1. [1]
  Hao Cai , Xiaoyan Wu , Lei Jiang , Feng Yu , Yuxiang Yang , Yan Li , Xian Zhang , Jian Liu , Zijian Li , Hong Bi . Lysosome-targeted carbon dots with a light-controlled nitric oxide releasing property for enhanced photodynamic therapy. Chinese Chemical Letters, 2024, 35(4): 108946-. doi: 10.1016/j.cclet.2023.108946
2. [2]
  Qi Wang , Yicong Gao , Feng Lu , Quli Fan . Preparation and Performance Characterization of the Second Near-Infrared Phototheranostic Probe: A New Design and Teaching Practice of Polymer Chemistry Comprehensive Experiment. University Chemistry, 2024, 39(11): 342-349. doi: 10.12461/PKU.DXHX202404141
3. [3]
  Yuanyi Zhou , Ke Ma , Jinfeng Liu , Zirun Zheng , Bo Hu , Yu Meng , Zhizhong Li , Mingshan Zhu . Is reactive oxygen species the only way for cancer inhibition over single atom nanomedicine? Autophagy regulation also works. Chinese Chemical Letters, 2024, 35(6): 109056-. doi: 10.1016/j.cclet.2023.109056
4. [4]
  Jianing He , Xiao Wang , Zijian Wang , Ruize Jiang , Ke Wang , Rui Zhang , Huilin Wang , Baokang Geng , Hongyi Gao , Shuyan Song , Hongjie Zhang . Investigation on Cu promotion effect on Ce-based solid solution-anchored Rh single atoms for three-way catalysis. Chinese Chemical Letters, 2025, 36(2): 109640-. doi: 10.1016/j.cclet.2024.109640
5. [5]
  Yanfei Liu , Yaqin Hu , Yifu Tan , Qiwen Chen , Zhenbao Liu . Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition. Chinese Chemical Letters, 2025, 36(1): 110289-. doi: 10.1016/j.cclet.2024.110289
6. [6]
  Gaojian Yang , Zhiyang Li , Rabia Usman , Zhu Chen , Yuan Liu , Song Li , Hui Chen , Yan Deng , Yile Fang , Nongyue He . DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer. Chinese Chemical Letters, 2025, 36(2): 109930-. doi: 10.1016/j.cclet.2024.109930
7. [7]
  Yifan LIU , Zhan ZHANG , Rongmei ZHU , Ziming QIU , Huan PANG . A three-dimensional flower-like Cu-based composite and its low-temperature calcination derivatives for efficient oxygen evolution reaction. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 979-990. doi: 10.11862/CJIC.20240008
8. [8]
  Junjie Wang , Yan Wang , Zhengdong Li , Changqiang Xie , Musammir Khan , Xingzhou Peng , Fabiao Yu . Triphenylamine-AIEgens photoactive materials for cancer theranostics. Chinese Chemical Letters, 2024, 35(6): 108934-. doi: 10.1016/j.cclet.2023.108934
9. [9]
  Ling Yang , Min Ren , Jie Wang , Liming He , Shanshan Wu , Shuai Yang , Wei Zhao , Hao Cheng , Xiaoming Zhou , Maling Gou . A non-viral gene therapy for melanoma by staphylococcal enterotoxin A. Chinese Chemical Letters, 2024, 35(5): 108822-. doi: 10.1016/j.cclet.2023.108822
10. [10]
  Huijie An , Chen Yang , Zhihui Jiang , Junjie Yuan , Zhongming Qiu , Longhao Chen , Xin Chen , Mutu Huang , Linlang Huang , Hongju Lin , Biao Cheng , Hongjiang Liu , Zhiqiang Yu . Luminescence-activated Pt(Ⅳ) prodrug for in situ triggerable cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109134-. doi: 10.1016/j.cclet.2023.109134
11. [11]
  Mengjuan Sun , Muye Zhou , Yifang Xiao , Hailei Tang , Jinhua Chen , Ruitao Zhang , Chunjiayu Li , Qi Ya , Qian Chen , Jiasheng Tu , Qiyue Wang , Chunmeng Sun . Reversibly size-switchable polyion complex micelles for antiangiogenic cancer therapy. Chinese Chemical Letters, 2024, 35(7): 109110-. doi: 10.1016/j.cclet.2023.109110
12. [12]
  Haijing Cui , Weihao Zhu , Chuning Yue , Ming Yang , Wenzhi Ren , Aiguo Wu . Recent progress of ultrasound-responsive titanium dioxide sonosensitizers in cancer treatment. Chinese Chemical Letters, 2024, 35(10): 109727-. doi: 10.1016/j.cclet.2024.109727
13. [13]
  Xiaofang Luo , Ye Wu , Xiaokun Zhang , Min Tang , Feiye Ju , Zuodong Qin , Gregory J Duns , Wei-Dong Zhang , Jiang-Jiang Qin , Xin Luan . Peptide-based strategies for overcoming multidrug-resistance in cancer therapy. Chinese Chemical Letters, 2025, 36(1): 109724-. doi: 10.1016/j.cclet.2024.109724
14. [14]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
15. [15]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
16. [16]
  Huijiao Fu , Peiqin Liang , Qianwen Chen , Yan Wang , Guang Li , Xuzi Cai , Shengtao Wang , Kun Chen , Shengying Shi , Zhiqiang Yu , Xuefeng Wang . COX-2 blocking therapy in cisplatin chemosensitization of ovarian cancer: An allicin-based nanomedicine approach. Chinese Chemical Letters, 2024, 35(8): 109241-. doi: 10.1016/j.cclet.2023.109241
17. [17]
  Yang Liu , Yan Liu , Kaiyin Yang , Zhiruo Zhang , Wenbo Zhang , Bingyou Yang , Hua Li , Lixia Chen . A selective HK2 degrader suppresses SW480 cancer cell growth by degrading HK2. Chinese Chemical Letters, 2024, 35(8): 109264-. doi: 10.1016/j.cclet.2023.109264
18. [18]
  Xiangqian Cao , Chenkai Yang , Xiaodong Zhu , Mengxin Zhao , Yilin Yan , Zhengnan Huang , Jinming Cai , Jingming Zhuang , Shengzhou Li , Wei Li , Bing Shen . Synergistic enhancement of chemotherapy for bladder cancer by photothermal dual-sensitive nanosystem with gold nanoparticles and PNIPAM. Chinese Chemical Letters, 2024, 35(8): 109199-. doi: 10.1016/j.cclet.2023.109199
19. [19]
  Qiang Li , Jiangbo Fan , Hongkai Mu , Lin Chen , Yongzhen Yang , Shiping Yu . Nucleus-targeting orange-emissive carbon dots delivery adriamycin for enhanced anti-liver cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108947-. doi: 10.1016/j.cclet.2023.108947
20. [20]
  Yihan Zhou , Duo Gao , Yaying Wang , Li Liang , Qingyu Zhang , Wenwen Han , Jie Wang , Chunliu Zhu , Xinxin Zhang , Yong Gan . Worm-like micelles facilitate the intestinal mucus diffusion and drug accumulation for enhancing colorectal cancer therapy. Chinese Chemical Letters, 2024, 35(6): 108967-. doi: 10.1016/j.cclet.2023.108967

Get Citation

PDF

XML

Metrics

PDF Downloads(8)
Abstract views(1038)
HTML views(146)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142