Citation: Jiafu Chang, Wenxin Lv, Jiahui Wu, Haiyin Li, Feng Li. Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification[J]. Chinese Chemical Letters, ;2021, 32(2): 775-778. doi: 10.1016/j.cclet.2020.05.041 shu

Simultaneous photoelectrochemical detection of dual microRNAs by capturing CdS quantum dots and methylene blue based on target-initiated strand displaced amplification

Jiafu Chang ^{a, b} ,
Wenxin Lv ^a ,
Jiahui Wu ^a ,
Haiyin Li ^{a, *,} ,
Feng Li ^{a, b, *,}

a.
College of Chemistry and Pharmaceutical Sciences, Qingdao Agricultural University, Qingdao 266109, China
b.
College of Chemistry, Chemical Engineering and Materials Science, Shandong Normal University, Ji'nan 250014, China

lihaiyin@qau.edu.cn

lifeng@qau.edu.cn

Received Date: 15 April 2020
Revised Date: 19 May 2020
Accepted Date: 28 May 2020
Available Online: 31 May 2020

Figures(4)

Herein, we propose a novel photoelectrochemical (PEC) biosensor for dual microRNAs (miRNAs) highly sensitive and simultaneous biosensing based on strand displaced amplification (SDA) reaction. The recognition of HmiR-21 and Hlet-7a by microRNA-21 and let-7a leads to their change in hairpin structures, subsequently initiating the immobilization of abundant CdS quantum dots (CdS QDs) and methylene blue (MB) based on SDA reaction. The immobilized CdS QDs and MB produce both high PEC currents under 430 nm light and 627 nm light illumination, respectively, and the generated PEC currents are closely relied on target miRNAs amounts. Thus, highly sensitive and simultaneous detection of microRNA-21 Herein, we propose a novel photoelectrochemical (PEC) biosensor for dual microRNAs (miRNAs) highly sensitive and simultaneous biosensing based on strand displaced amplification (SDA) reaction. The recognition of H_miR-21 and H_let-7a by microRNA-21 and let-7a leads to their change in hairpin structures, subsequently initiating the immobilization of abundant CdS quantum dots (CdS QDs) and methylene blue (MB) based on SDA reaction. The immobilized CdS QDs and MB produce both high PEC currents under 430 nm light and 627 nm light illumination, respectively, and the generated PEC currents are closely relied on target miRNAs amounts. Thus, highly sensitive and simultaneous detection of microRNA-21 and let-7a was readily achieved with detection limit at 6.6 fmol/L and 15.4 fmol/L based on 3σ, respectively. Further, this PEC biosensor was applied in simultaneous analysis of miRNA-21 and let-7a in breast cancer patient's serum with acceptable results. We expect this biosensor will find more useful application in diagnosis of miRNA-related diseases.
- PEC,
- CdS QDs,
- Methylene blue,
- Multiple microRNAs,
- Strand displaced amplification,
- Simultaneous biosensing
1. [1]
  Y.Q. Xia, R.L. Zhang, Z.L. Wang, J. Tian, X.Y. Chen, Chem. Soc. Rev. 46 (2017) 2824-2843. doi: 10.1039/C6CS00675B
2. [2]
  B. Wu, C. Eliscovich, Y.J. Yoon, R.H. Singer, Science 352 (2016) 1430-1435. doi: 10.1126/science.aaf1084
3. [3]
  R.J. Deng, L.H. Tang, Q.Q. Tian, et al., Angew. Chem. Int. Ed. 126 (2014) 2421-2425. doi: 10.1002/ange.201309388
4. [4]
  X. Miao, Z. Cheng, H. Ma, et al., Anal. Chem. 90 (2018) 1098-1103. doi: 10.1021/acs.analchem.7b01991
5. [5]
  J.F. Chang, X. Wang, J. Wang, H.Y. Li, F. Li, Anal. Chem. 91 (2019) 3604-3610. doi: 10.1021/acs.analchem.8b05599
6. [6]
  W. Pan, B. Liu, X.N. Gao, et al., Nanoscale 10 (2018) 14264-14271. doi: 10.1039/C8NR04106G
7. [7]
  Y.H. Yuan, Y.D. Wu, B.Z. Chi, et al., Biosens. Bioelectron. 97 (2017) 325-331. doi: 10.1016/j.bios.2017.06.022
8. [8]
  A. VÃ¡zquez-Guardado, S. Barkam, M. Peppler, et al., Nano Lett. 19 (2019) 449-454. doi: 10.1021/acs.nanolett.8b04253
9. [9]
  J.R. Wang, Z.X. Lu, H.L. Tang, et al., Anal. Chem. 89 (2017) 10834-10840. doi: 10.1021/acs.analchem.7b02342
10. [10]
  S. Wu, C. Li, H. Shi, Y. Huang, G.X. Li, Anal. Chem. 90 (2018) 9929-9935. doi: 10.1021/acs.analchem.8b02127
11. [11]
  W.J. Wang, Y.L. Cai, B.C. Li, et al., Chin. Chem. Lett. 29 (2018) 111-114. doi: 10.1016/j.cclet.2017.05.009
12. [12]
  Q. Zhou, G.H. Li, K.Y. Chen, et al., Anal. Chem. 92 (2020) 983-990. doi: 10.1021/acs.analchem.9b03915
13. [13]
  Y. Zhang, N. Hao, Z. Zhou, et al., Chem. Commun. 53 (2017) 5810-5813. doi: 10.1039/C7CC01582H
14. [14]
  Q. Zhou, H. Xue, Y.Y. Zhang, et al., ACS Sens. 3 (2018) 1385-1391. doi: 10.1021/acssensors.8b00307
15. [15]
  Y.Q. Lv, Z.X. Zhou, Y.F. Shen, et al., ACS Sens. 3 (2018) 1362-1367. doi: 10.1021/acssensors.8b00292
16. [16]
  S.W. Yang, J. Sun, P. He, et al., Chem. Mater. 27 (2015) 2004-2011. doi: 10.1021/acs.chemmater.5b00112
17. [17]
  Q. Liu, D.B. Zhu, M.L. Guo, Y. Yu, Y.J. Cao, Chin. Chem. Lett. 30 (2019) 1639-1642. doi: 10.1016/j.cclet.2019.05.058
18. [18]
  N. Zhang, L. Zhang, Y.F. Ruan, et al., Biosens. Bioelectron. 94 (2017) 207-218. doi: 10.1016/j.bios.2017.03.011
19. [19]
  G.N. Cai, Z.Z. Yu, R.R. Ren, D.P. Tang, ACS Sens. 3 (2018) 632-639. doi: 10.1021/acssensors.7b00899
20. [20]
  Q. Hao, X.N. Shan, J.P. Lei, et al., Chem. Sci. 7 (2016) 774-780. doi: 10.1039/C5SC03336E
21. [21]
  T. Hou, N.N. Xu, W.X. Wang, L. Ge, F. Li, Anal. Chem. 90 (2018) 9591-9597. doi: 10.1021/acs.analchem.8b02523
22. [22]
  Y.N. Zheng, W.B. Liang, C.Y. Xiong, et al., Anal. Chem. 89 (2017) 9445-9451. doi: 10.1021/acs.analchem.7b02270
23. [23]
  F.C. Simmel, B. Yurke, H.R. Singh, Chem. Rev. 119 (2019) 6326-6369.
1. [1]
  Zongyi Huang , Cheng Guo , Quanxing Zheng , Hongliang Lu , Pengfei Ma , Zhengzhong Fang , Pengfei Sun , Xiaodong Yi , Zhou Chen . Efficient photocatalytic biomass-alcohol conversion with simultaneous hydrogen evolution over ultrathin 2D NiS/Ni-CdS photocatalyst. Chinese Chemical Letters, 2024, 35(7): 109580-. doi: 10.1016/j.cclet.2024.109580
2. [2]
  Yiyang Shen , Zhen Zhang , Ruyi Liang , Tongbo Wu . Unraveling the interplay of DNAzyme and interfacial factors for enhanced biosensing. Chinese Chemical Letters, 2024, 35(12): 109638-. doi: 10.1016/j.cclet.2024.109638
3. [3]
  Erzhuo Cheng , Yunyi Li , Wei Yuan , Wei Gong , Yanjun Cai , Yuan Gu , Yong Jiang , Yu Chen , Jingxi Zhang , Guangquan Mo , Bin Yang . Galvanostatic method assembled ZIFs nanostructure as novel nanozyme for the glucose oxidation and biosensing. Chinese Chemical Letters, 2024, 35(9): 109386-. doi: 10.1016/j.cclet.2023.109386
4. [4]
  Chuang LIU , Lichao SUN , Qingfeng ZHANG . Chiral inorganic nanocatalysts for electrochemical and enzyme-mimicked biosensing. Chinese Journal of Inorganic Chemistry, 2025, 41(1): 59-78. doi: 10.11862/CJIC.20240406
5. [5]
  Ying Xu , Chengying Shen , Hailong Yuan , Wei Wu . Mapping multiple phases in curcumin binary solid dispersions by fluorescence contrasting. Chinese Chemical Letters, 2024, 35(9): 109324-. doi: 10.1016/j.cclet.2023.109324
6. [6]
  Zhi Wang , Lingpeng Yan , Yelin Hao , Jingxia Zheng , Yongzhen Yang , Xuguang Liu . Highly efficient and photothermally stable CDs@ZIF-8 for laser illumination. Chinese Chemical Letters, 2024, 35(10): 109430-. doi: 10.1016/j.cclet.2023.109430
7. [7]
  Hui Liu , Xiangyang Tang , Zhuang Cheng , Yin Hu , Yan Yan , Yangze Xu , Zihan Su , Futong Liu , Ping Lu . Constructing multifunctional deep-blue emitters with weak charge transfer excited state for high-performance non-doped blue OLEDs and single-emissive-layer hybrid white OLEDs. Chinese Chemical Letters, 2024, 35(10): 109809-. doi: 10.1016/j.cclet.2024.109809
8. [8]
  Yang Qin , Jiangtian Li , Xuehao Zhang , Kaixuan Wan , Heao Zhang , Feiyang Huang , Limei Wang , Hongxun Wang , Longjie Li , Xianjin Xiao . Toeless and reversible DNA strand displacement based on Hoogsteen-bond triplex. Chinese Chemical Letters, 2024, 35(5): 108826-. doi: 10.1016/j.cclet.2023.108826
9. [9]
  Jiayu Xu , Meng Li , Baoxia Dong , Ligang Feng . Fully fluorinated hybrid zeolite imidazole/Prussian blue analogs with combined advantages for efficient oxygen evolution reaction. Chinese Chemical Letters, 2024, 35(6): 108798-. doi: 10.1016/j.cclet.2023.108798
10. [10]
  Pingping Wang , Huixian Miao , Kechuan Sheng , Bin Wang , Fan Feng , Xuankun Cai , Wei Huang , Dayu Wu . Efficient blue-light-excitable copper(Ⅰ) coordination network phosphors for high-performance white LEDs. Chinese Chemical Letters, 2024, 35(4): 108600-. doi: 10.1016/j.cclet.2023.108600
11. [11]
  Xianping Du , Ying Huang , Chen Chen , Zhenhe Feng , Meng Zong . Encapsulating Si particles in multiple carbon shells with pore-rich for constructing free-standing anodes of lithium storage. Chinese Chemical Letters, 2024, 35(12): 109990-. doi: 10.1016/j.cclet.2024.109990
12. [12]
  Liwen Wang , Boyang Wang , Siyu Lu , Shubo Lv , Xiaoli Qu . High quantum yield yellow emission carbon dots for the construction of blue light blocking films. Chinese Chemical Letters, 2025, 36(2): 110497-. doi: 10.1016/j.cclet.2024.110497
13. [13]
  Jia-Mei Qin , Xue Li , Wei Lang , Fu-Hao Zhang , Qian-Yong Cao . An AIEgen nano-assembly for simultaneous detection of ATP and H₂S. Chinese Chemical Letters, 2024, 35(6): 108925-. doi: 10.1016/j.cclet.2023.108925
14. [14]
  Gang Lang , Jing Feng , Bo Feng , Junlan Hu , Zhiling Ran , Zhiting Zhou , Zhenju Jiang , Yunxiang He , Junling Guo . Supramolecular phenolic network-engineered C–CeO₂ nanofibers for simultaneous determination of isoniazid and hydrazine in biological fluids. Chinese Chemical Letters, 2024, 35(6): 109113-. doi: 10.1016/j.cclet.2023.109113
15. [15]
  Dan Luo , Jinya Tian , Jianqiao Zhou , Xiaodong Chi . Anthracene-bridged "Texas-sized" box for the simultaneous detection and uptake of tryptophan. Chinese Chemical Letters, 2024, 35(9): 109444-. doi: 10.1016/j.cclet.2023.109444
16. [16]
  Tong Su , Yue Wang , Qizhen Zhu , Mengyao Xu , Ning Qiao , Bin Xu . Multiple conductive network for KTi₂(PO₄)₃ anode based on MXene as a binder for high-performance potassium storage. Chinese Chemical Letters, 2024, 35(8): 109191-. doi: 10.1016/j.cclet.2023.109191
17. [17]
  Yi Cao , Xiaojiao Ge , Yuanyuan Wei , Lulu He , Aiguo Wu , Juan Li . Tumor microenvironment-activatable neuropeptide-drug conjugates enhanced tumor penetration and inhibition via multiple delivery pathways and calcium deposition. Chinese Chemical Letters, 2024, 35(4): 108672-. doi: 10.1016/j.cclet.2023.108672
18. [18]
  Yuan CONG , Yunhao WANG , Wanping LI , Zhicheng ZHANG , Shuo LIU , Huiyuan GUO , Hongyu YUAN , Zhiping ZHOU . Construction and photocatalytic properties toward rhodamine B of CdS/Fe₃O₄ heterojunction. Chinese Journal of Inorganic Chemistry, 2024, 40(11): 2241-2249. doi: 10.11862/CJIC.20240219
19. [19]
  Kuangdi Luo , Yang Qin , Xuehao Zhang , Hanxu Ji , Heao Zhang , Jiangtian Li , Xianjin Xiao , Xinyu Wang . Regulable toehold lock for the effective control of strand displacement reaction sequence and circuit leakage. Chinese Chemical Letters, 2024, 35(7): 109104-. doi: 10.1016/j.cclet.2023.109104
20. [20]
  Zengchao Guo , Weiwei Liu , Tengfei Liu , Jinpeng Wang , Hui Jiang , Xiaohui Liu , Yossi Weizmann , Xuemei Wang . Engineered exosome hybrid copper nanoscale antibiotics facilitate simultaneous self-assembly imaging and elimination of intracellular multidrug-resistant superbugs. Chinese Chemical Letters, 2024, 35(7): 109060-. doi: 10.1016/j.cclet.2023.109060

Get Citation

PDF

XML

Metrics

PDF Downloads(10)
Abstract views(1038)
HTML views(167)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142