Citation: Ji Dongqing, Ying Zi, Zhang Yuan, Chen Wei, Janyasupab Metini, Gao Xinghua, Feng Lingyan, Wen Weijia. RhIr@MoS₂ nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum[J]. Chinese Chemical Letters, ;2020, 31(8): 2115-2118. doi: 10.1016/j.cclet.2019.12.029 shu

RhIr@MoS₂ nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum

Ji Dongqing ^a,b ,
Ying Zi ^a ,
Zhang Yuan ^a,b,*, ,
Chen Wei ^c ,
Janyasupab Metini ^d ,
Gao Xinghua ^a ,
Feng Lingyan ^a,*, ,
Wen Weijia ^a

a.
Materials Genome Institute, Shanghai University, Shanghai 200444, China
b.
State Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China
c.
Department of Emergency, Tongji Hospital, Tongji University School of Medicine, Shanghai 200065, China
d.
Department of Electronics Engineering, King Mongkut's Institute of Technology Ladkrabang, Bangkok 10520, Thailand

zhangyuan@shu.edu.cn

lingyanfeng@t.shu.edu.cn

Received Date: 17 November 2019
Revised Date: 17 December 2019
Accepted Date: 19 December 2019
Available Online: 20 December 2019

Figures(4)

Dihydronicotinamide adenine dinucleotide (NADH) is an important enzyme in all living cells, which is found to be abnormally expressed in cancer cells. Since it is redox-active, an electrochemical detection method would be suitable for monitoring its concentration in biological fluids. Here we present a strategy for specific determination of NADH in real human serum by using RhIr@MoS₂ nanohybrids based microsensor. To implement the protocol, RhIr nanocrysrals are in-situ grown onto MoS₂ interlayers forming a nanohybrid structure (RhIr@MoS₂). After being locally deposited on an electrochemical microsensor, it could be used for the analysis of NADH. The developed RhIr@MoS₂ nanohybrids based microsensor possesses the ability for analyzing NADH at the applied potential of 0.07 V (much lower than most reported values). The detection limit is evaluated as low as 1 nmol/L even in bovine serum albumin (BSA) media. In addition, the sampling analysis of human serum from cancer patients and health controls shows that the microsensor displays good diagnostic sensitivity and specificity, illustrating that this developed detection technique is a relatively accurate method for measuring NADH in biological fluids. The proposed electrochemical microsensor assay also owns the benefits of convenience, disposable and easy processing, which make it a great possibility for future point-of-care cancer diagnosis.
- MoS₂,
- Nanohybrid,
- Electrochemical microsensor,
- Point-of-care (POC) test,
- Cancer diagnosis
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沈阳化工大学材料科学与工程学院沈阳 110142

RhIr@MoS2 nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum

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通讯作者: 陈斌, bchen63@163.com

RhIr@MoS₂ nanohybrids based disposable microsensor for the point-of-care testing of NADH in real human serum