Citation: Zhang Hailiang, Xu Pengfei, Zhang Xintong, Cao Xiaozheng, Han Wenxiu, Liu Meihui, Liu Xiaohui, Zeng Wenbin. Au nanoparticles based ultra-fast "Turn-On" fluorescent sensor for detection of biothiols and its application in living cell imaging[J]. Chinese Chemical Letters, ;2020, 31(5): 1083-1086. doi: 10.1016/j.cclet.2019.10.005 shu

Au nanoparticles based ultra-fast "Turn-On" fluorescent sensor for detection of biothiols and its application in living cell imaging

Zhang Hailiang ^a,b ,
Xu Pengfei ^a,b ,
Zhang Xintong ^a,c ,
Cao Xiaozheng ^a ,
Han Wenxiu ^a,b ,
Liu Meihui ^a ,
Liu Xiaohui ^a ,
Zeng Wenbin ^a,*,

a.
Xiangya School of Pharmaceutical Sciences, Central South University, Changsha 410006, China
b.
Institute of Clinical Pharmacy and Pharmacology, Jining First People's Hospital, Jining Medical University, Jining 272000, China
c.
Yidu Central Hospital of Weifang, Weifang 261000, China

wbzeng@hotmail.com

Received Date: 25 August 2019
Revised Date: 8 October 2019
Accepted Date: 9 October 2019
Available Online: 14 October 2019

Figures(4)

Au or other metal nanostructures have the ability to strongly quench the fluorescence of fluorophores. This feature has made AuNP-conjugates attractive for the construction of platforms for various bioanalytes to overcome the limitations of small molecule fluorophores (poor solubility, long reaction time). In this paper, an ultrafast "Turn-On" fluorescent sensor for biothiols was constructed. The sensor is based on the fluorescent resonance energy transfer (FRET) effect between the fluorophore (PN) and AuNPs, which effectively quenches the fluorescence of the fluorophore. In the presence of thiols, PN is displaced and released from AuNP surfaces, and thus, the fluorescence is rapidly restored. The sensor features appreciable water solubility and ultrafast response time (a few seconds for Cys). In addition, it exhibits high selectivity and a detection limit as low as 12 nmol/L for Hcy. Moreover, the sensor presents good biocompatibility and has been successfully applied for imaging biothiols in living cells.
- Gold nanoparticle,
- FRET,
- Biothiols,
- Sensor,
- Fluorescent imaging
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