Citation: Zheng Yong, Wu Fangming, Ling Shenglong, Li Jia-Bin, Tian Changlin. Total chemical synthesis of bivalently modified H3 by improved three-segment native chemical ligation[J]. Chinese Chemical Letters, ;2020, 31(5): 1267-1270. doi: 10.1016/j.cclet.2019.09.038 shu

Total chemical synthesis of bivalently modified H3 by improved three-segment native chemical ligation

Zheng Yong ^a,b ,
Wu Fangming ^a,b ,
Ling Shenglong ^a,b ,
Li Jia-Bin ^c,**, ,
Tian Changlin ^a,b,*,

a.
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China
b.
School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
c.
Jiangsu Key Laboratory of Neuropsychiatric Diseases and College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China

^**

lijiabin@ustc.edu.cn

cltian@ustc.edu.cn

Received Date: 21 August 2019
Revised Date: 17 September 2019
Accepted Date: 18 September 2019
Available Online: 19 September 2019

Figures(4)

The H3 bivalent modifications of trimethylation at Lys9 and acetylation at Lys18 (H3-K9Me3-K18Ac) were identified to collectively recruit TRIM33 in the nodal signaling pathway. To understand the underlying mechanism of TRIM33 recruitment, the nucleosome core particles (NCPs) containing full-length H3-K9Me3-K18Ac were indispensable samples. Herein we developed a pseudo dipeptide strategy to efficiently prepare peptide segments, facilitating the chemical synthesis of H3-K9Me3-K18Ac at a tens of milligram scale. The synthetic H3-K9Me3-K18Ac was then examined by CD spectroscopy, which demonstrated a prominent shift compared to recombinant H3. Finally, bivalently modified NCPs were assembled and verified by gel mobility shift assay with good homogeneity.
- Chemical protein synthesis,
- Native chemical ligation,
- Histone H3,
- Methylation,
- Acetylation,
- Nucleosome core particles
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