Citation: Pu Ximing, Zhou Xingxing, Huang Zhongbing, Yin Guangfu, Chen Xianchun. Fabrication of extracellular matrix-coated conductive polypyrrolepoly(L-lactide) fiber-films and their synergistic effect with (nerve growth factor)/(epidermal growth factor) on neurites growth[J]. Chinese Chemical Letters, ;2020, 31(5): 1141-1146. doi: 10.1016/j.cclet.2019.07.002 shu

Fabrication of extracellular matrix-coated conductive polypyrrolepoly(L-lactide) fiber-films and their synergistic effect with (nerve growth factor)/(epidermal growth factor) on neurites growth

College of Materials Science and Engineering, Sichuan University, Chengdu 610065, China

zbhuang@scu.edu.cn

Received Date: 6 April 2019
Revised Date: 22 June 2019
Accepted Date: 1 July 2019
Available Online: 2 July 2019

Figures(4)

Non-nerve cell-derived extracellular matrix (ECM) was coated on the aligned porous polypyrrole-poly(L-lactide) (PPy-PLLA) fiber-films with the conductivity of ~12 mS/m via L929 cells culture and lysing, resulting in~10% increase of PC12 cells attachment and 26 μm increase of neurites length. The neurite length of ~149 μm in EGF/NGF group (optimal concentration radio of 12.5/50 (ng/mL)) on aligned and ECM-conjugated fiber-films was significantly larger than ~94 μm in only NGF group (50 ng/mL), confirming the synergy of EGF, NGF and aligned ECM-conjuaged PPy-PLLA fibers. When differentiated PC12 cells were exerted electrical stimulation (ES) of 100 mV/cm for 4 h/day in 2 day through ECM-PPyPLLA fiber-films, their neurite length reached to ~251 μm, significantly larger than ~149 μm of group without ES, due to the higer expression of related neural proteins in ES group. A simple mechanism was proposed to analyze synergistical effect of ECM, EGF, NGF on axons adhesion and elongation along the aligned ECM-coated fibers under ES condition.
- Extracellular matrix,
- Axonal growth,
- Conducting PPy-PLLA fiber-film,
- Nerve growth factor,
- Epidermal growth factor,
- Electrical stimulation
1. [1]
  L. Tian, M.P. Prabhakaran, S. Regenerative, Biomaterials 2(2015) 31-45.
2. [2]
  H. Shin, S. Jo, A.G. Mikos, Biomaterials 24(2003) 4353-4364. doi: 10.1016/S0142-9612(03)00339-9
3. [3]
  X. Wen, P.A. Tresco, Biomaterials 27(2006) 3800-3809. doi: 10.1016/j.biomaterials.2006.02.036
4. [4]
  M.B. Runge, M. Dadsetan, J. Baltrusaitis, et al., Biomaterials 31(2010) 5916-5926. doi: 10.1016/j.biomaterials.2010.04.012
5. [5]
  F. Yang, R. Murugan, S. Wang, S. Ramakrishna, Biomaterials 26(2005) 2603-2610. doi: 10.1016/j.biomaterials.2004.06.051
6. [6]
  N. Bhardwaj, S.C. Kundu, Biotech. Adv. 28(2010) 325-347. doi: 10.1016/j.biotechadv.2010.01.004
7. [7]
  S.H. Lim, H.Q. Mao, Adv. Drug Deliv. Rev. 61(2009) 1084-1096. doi: 10.1016/j.addr.2009.07.011
8. [8]
  Y. Yu, X. Lü, F. Ding, J. Biomed. Nanotech. 11(2015) 816-827. doi: 10.1166/jbn.2015.1973
9. [9]
  J. Xie, W. Liu, M.R. MacEwan, P.C. Bridgman, Y. Xia, ACS Nano 8(2014) 1878-1885. doi: 10.1021/nn406363j
10. [10]
  N.K. Guimard, N. Gomez, C.E. Schmidt, Prog. Polym. Sci. 32(2007) 876-921. doi: 10.1016/j.progpolymsci.2007.05.012
11. [11]
  P.M. George, A.W. Lyckman, et al., Biomaterials 26(2005) 3511-3519. doi: 10.1016/j.biomaterials.2004.09.037
12. [12]
  C.E. Schmidt, V.R. Shastri, J.P. Vacanti, R. Langer, Proc. Natl. Acad. Sci. U. S. A. 94(1997) 8948-8953. doi: 10.1073/pnas.94.17.8948
13. [13]
  L. Jin, Z.Q. Feng, M.L. Zhu, et al., J. Biomed. Nanotech. 8(2012) 779-785. doi: 10.1166/jbn.2012.1443
14. [14]
  J. Zhang, K. Qiu, B. Sun, et al., J. Mater. Chem. B:Mater. Biol. Med. 2(2014) 7945-7954. doi: 10.1039/C4TB01185F
15. [15]
  Y. Lu, T. Li, X. Zhao, et al., Biomaterials 31(2010) 5169-5181. doi: 10.1016/j.biomaterials.2010.03.022
16. [16]
  D. Blumberg, M. Radeke, S. Feinstein, J. Neurosci. Res. 41(1995) 628-639. doi: 10.1002/jnr.490410509
17. [17]
  D.R. Tyson, S. Larkin, Y. Hamai, R.A. Bradshaw, Int. J. Dev. Neurosci. 21(2003) 63-74. doi: 10.1016/S0736-5748(02)00139-9
18. [18]
  H. Lu, T. Hoshiba, N. Kawazoe, et al., Biomaterials 32(2011) 9658-9666. doi: 10.1016/j.biomaterials.2011.08.091
19. [19]
  K. Hozumi, N. Suzuki, P.K. Nielsen, M. Nomizu, Y. Yamada, J. Biol. Chem. 281(2006) 32929-32940. doi: 10.1074/jbc.M605708200
20. [20]
  R. Timpl, H. Rohde, P.G. Robey, et al., J. Biol. Chem. 254(1979) 9933-9937.
21. [21]
  M.D. Pierschbacher, E. Ruoslahti, Nature 309(1983) 30-33.
22. [22]
  V.J. Mukhatyar, M. Salmeron-Sanchez, S. Rudra, et al., Biomaterials 32(2011) 3958-3968. doi: 10.1016/j.biomaterials.2011.02.015
23. [23]
  A. Alovskaya, T. Alekseeva, J. Phillips, V. King, R. Brown, Top Tissue Eng. 3(2007) 1-26.
24. [24]
  G. Fabregat, B. Teixeira-Dias, L.J. del Valle, et al., ACS Appl. Mater. Interf. 6(2014) 11940-11954. doi: 10.1021/am503904h
25. [25]
  R. Levi-Montalcini, Prog. Brain Res. 45(2011) 235-256.
26. [26]
  T. Dvir, B.P. Timko, D.S. Kohane, R. Langer, Nat. Nanotechn. 6(2011) 13-22. doi: 10.1038/nnano.2010.246
27. [27]
  Y.Y. Wu, R.A. Bradshaw, J. Biol. Chem. 271(1996) 13033-13039. doi: 10.1074/jbc.271.22.13033
28. [28]
  R.S. Morrison, H.I. Kornblum, F.M. Leslie, R.A. Bradshaw, Science 238(1987) 72-75. doi: 10.1126/science.3498986
29. [29]
  N.P. Tsai, Y.C. Tsui, J.E. Pintar, H.H. Loh, L.N. Wei, P. Natl. Acad. Sci. U. S. A. 107(2010) 3216-3221. doi: 10.1073/pnas.0912367107
30. [30]
  G.J.R. Delcroix, K.M. Curtis, P.C. Schiller, C.N. Montero-Menei, Differentiation 80(2010) 213-227. doi: 10.1016/j.diff.2010.07.001
31. [31]
  H. Yan, J. Schlessinger, M.V. Chao, Science 252(1991) 561-563. doi: 10.1126/science.1850551
32. [32]
  J.Y. Lee, C. Bashur, C. Milroy, et al., IEEE T. Nano Biosci. 11(2012) 15-21. doi: 10.1109/TNB.2011.2159621
33. [33]
  K. Alheim, C. Andersson, S. Tingsborg, et al., Proc. Natl. Acad. Sci. U. S. A. 88(1991) 9302-9306. doi: 10.1073/pnas.88.20.9302
34. [34]
  M.P. Prabhakaran, J.R. Venugopal, S. Ramakrishna, Biomaterials 30(2009) 4996-5003. doi: 10.1016/j.biomaterials.2009.05.057
35. [35]
  J. Huang, L. Lu, X. Hu, et al., Neurorehabil. Neural Repair 24(2010) 736-745. doi: 10.1177/1545968310368686
36. [36]
  J. Zhang, M. Li, E.T. Kang, K.G. Neoh, Acta Biomater. 32(2016) 46-56. doi: 10.1016/j.actbio.2015.12.024
37. [37]
  X. Zhou, A. Yang, Z. Huang, et al., Colloids Surf. B:Biointerfaces 149(2017) 217-225. doi: 10.1016/j.colsurfb.2016.10.014
38. [38]
  J.D. Foley, E.W. Grunwald, P.F. Nealey, C.J. Murphy, Biomaterials 26(2005) 3639-3644. doi: 10.1016/j.biomaterials.2004.09.048
39. [39]
  J. Zeng, Z. Huang, G. Yin, et al., Colloids Surf. B:Biointerfaces 110(2013) 450-457. doi: 10.1016/j.colsurfb.2013.05.012
40. [40]
  J. Ding, J. Zhang, J. Li, et al., Prog. Polym. Sci. 90(2019) 1-34. doi: 10.1016/j.progpolymsci.2019.01.002
41. [41]
  J. Li, W. Xu, D. Li, et al., ACS Nano 12(2018) 6685-6699. doi: 10.1021/acsnano.8b01729
42. [42]
  J. Li, W. Xu, J. Chen, et al., ACS Biomater. Sci. Eng. 4(2018) 2026-2036. doi: 10.1021/acsbiomaterials.7b00605
43. [43]
  J. Li, X. Feng, J. Shi, et al., ACS Omega 3(2018) 2715-2723. doi: 10.1021/acsomega.8b00090
44. [44]
  S. Li, S. Dong, W. Xu, et al., Adv. Sci. 5(2018) 1700527. doi: 10.1002/advs.201700527
45. [45]
  A. Yang, Z. Huang, G. Yin, X. Pu, Colloids Surf. B:Biointerfaces 134(2015) 469-474. doi: 10.1016/j.colsurfb.2015.07.028
46. [46]
  J.Y. Lee, C.A. Bashur, A.S. Goldstein, C.E. Schmidt, Biomaterials 30(2009) 4325-4335. doi: 10.1016/j.biomaterials.2009.04.042
47. [47]
  J. Xie, M.R. Macewan, S.M. Willerth, et al., Adv. Funct. Mater. 19(2009) 2312-2318. doi: 10.1002/adfm.200801904
48. [48]
  H. Aubin, J.W. Nichol, C.B. Hutson, et al., Biomaterials 31(2010) 6941-6951. doi: 10.1016/j.biomaterials.2010.05.056
49. [49]
  U. Malhotra, S. Maity, A. Chatterjee, J. Appl. Polym. Sci. 132(2015) 41336.
50. [50]
  X. Feng, J. Li, X. Zhang, et al., J. Control. Release 302(2019) 19-41. doi: 10.1016/j.jconrel.2019.03.020
51. [51]
  X. Chen, X. Zhuang, C. Fu, et al., Curr. Pharm. Design 21(2015) 1960-1966. doi: 10.2174/1381612821666150302152420
52. [52]
  Z. Jin, T. Zheng, E. Alarçin, et al., Small 13(2017) 1701949. doi: 10.1002/smll.201701949
53. [53]
  F. Yang, R. Murugan, S. Wang, S. Ramakrishna, Biomaterials 26(2005) 2603-2610. doi: 10.1016/j.biomaterials.2004.06.051
54. [54]
  J.M. Corey, D.Y. Lin, K.B. Mycek, et al., J. Biomed. Mater. Res. A 83(2007) 636-645.
55. [55]
  G. Bartosz, Biochem. Pharmacol. 77(2009) 1303-1315. doi: 10.1016/j.bcp.2008.11.009
56. [56]
  S.R. Heidemann, Int. Rev. Cytol. 165(1996) 235-296. doi: 10.1016/S0074-7696(08)62224-X
57. [57]
  N. Patel, M.M. Poo, J. Neurosci. 2(1982) 483-496. doi: 10.1523/JNEUROSCI.02-04-00483.1982
58. [58]
  Y. Sun, H. Shan, J. Wang, et al., J. Biomed. Nanotech. 15(2019) 939-950. doi: 10.1166/jbn.2019.2745
59. [59]
  S. Wang, Z. Zhang, D. Jiang, et al., Polymers 8(2016) 200. doi: 10.3390/polym8050200
60. [60]
  Z. Jin, T. Zheng, E. Alarçin, et al., Small 13(2017) 1701949. doi: 10.1002/smll.201701949
61. [61]
  J. Zhang, H. Liu, J.X. Ding, et al., RSC Adv. 5(2015) 32604-32608. doi: 10.1039/C5RA05530J
62. [62]
  D.J. Goldberg, D.W. Burmeister, J. Cell Biol. 103(1986) 1921-1931. doi: 10.1083/jcb.103.5.1921
63. [63]
  D. Johnson, A. Lanahan, C.R. Buck, et al., Cell 47(1986) 545-554. doi: 10.1016/0092-8674(86)90619-7
64. [64]
  P. Lamoureux, S.R. Heidemann, N.R. Martzke, K.E. Miller, Dev. Neurobiol. 70(2010) 135-149. doi: 10.1002/dneu.20764
65. [65]
  P.R. Chen, M.H. Chen, F.H. Lin, W.Y. Su, Biomaterials 26(2005) 6579-6587. doi: 10.1016/j.biomaterials.2005.03.037
1. [1]
  Xiongbo Song , Jinwen Xiao , Juan Wu , Li Sun , Long Chen . Decellularized amniotic membrane promotes the anti-inflammatory response of macrophages via PI3K/AKT/HIF-1α pathway. Chinese Chemical Letters, 2025, 36(1): 109844-. doi: 10.1016/j.cclet.2024.109844
2. [2]
  Yang Liu , Yan Liu , Kaiyin Yang , Zhiruo Zhang , Wenbo Zhang , Bingyou Yang , Hua Li , Lixia Chen . A selective HK2 degrader suppresses SW480 cancer cell growth by degrading HK2. Chinese Chemical Letters, 2024, 35(8): 109264-. doi: 10.1016/j.cclet.2023.109264
3. [3]
  Zhijia Zhang , Shihao Sun , Yuefang Chen , Yanhao Wei , Mengmeng Zhang , Chunsheng Li , Yan Sun , Shaofei Zhang , Yong Jiang . Epitaxial growth of Cu_2-xSe on Cu (220) crystal plane as high property anode for sodium storage. Chinese Chemical Letters, 2024, 35(7): 108922-. doi: 10.1016/j.cclet.2023.108922
4. [4]
  Wenhao Feng , Chunli Liu , Zheng Liu , Huan Pang . In-situ growth of N-doped graphene-like carbon/MOF nanocomposites for high-performance supercapacitor. Chinese Chemical Letters, 2024, 35(12): 109552-. doi: 10.1016/j.cclet.2024.109552
5. [5]
  Chuyu Huang , Zhishan Liu , Linping Zhao , Zuxiao Chen , Rongrong Zheng , Xiaona Rao , Yuxuan Wei , Xin Chen , Shiying Li . Metal-coordinated oxidative stress amplifier to suppress tumor growth combined with M2 macrophage elimination. Chinese Chemical Letters, 2024, 35(12): 109696-. doi: 10.1016/j.cclet.2024.109696
6. [6]
  Guizhi Zhu , Junrui Tan , Longfei Tan , Qiong Wu , Xiangling Ren , Changhui Fu , Zhihui Chen , Xianwei Meng . Growth of CeCo-MOF in dendritic mesoporous organosilica as highly efficient antioxidant for enhanced thermal stability of silicone rubber. Chinese Chemical Letters, 2025, 36(1): 109669-. doi: 10.1016/j.cclet.2024.109669
7. [7]
  Ruonan Yang , Jiajia Li , Dongmei Zhang , Xiuqi Zhang , Xia Li , Han Yu , Zhanhu Guo , Chuanxin Hou , Gang Lian , Feng Dang . Grain-refining Co_0.85Se@CNT cathode catalyst with promoted Li₂O₂ growth kinetics for lithium-oxygen batteries. Chinese Chemical Letters, 2024, 35(12): 109595-. doi: 10.1016/j.cclet.2024.109595
8. [8]
  Mengxiang Zhu , Tao Ding , Yunzhang Li , Yuanjie Peng , Ruiping Liu , Quan Zou , Leilei Yang , Shenglei Sun , Pin Zhou , Guosheng Shi , Dongting Yue . Graphene controlled solid-state growth of oxygen vacancies riched V₂O₅ catalyst to highly activate Fenton-like reaction. Chinese Chemical Letters, 2024, 35(12): 109833-. doi: 10.1016/j.cclet.2024.109833
9. [9]
  Gaojian Yang , Zhiyang Li , Rabia Usman , Zhu Chen , Yuan Liu , Song Li , Hui Chen , Yan Deng , Yile Fang , Nongyue He . DNA walker induced "signal on" fluorescence aptasensor strategy for rapid and sensitive detection of extracellular vesicles in gastric cancer. Chinese Chemical Letters, 2025, 36(2): 109930-. doi: 10.1016/j.cclet.2024.109930
10. [10]
  Yuqing Ding , Zhiying Yi , Zhihui Wang , Hongyu Chen , Yan Zhao . Liquid nitrogen post-treatment for improved aggregation and electrical properties in organic semiconductors. Chinese Chemical Letters, 2024, 35(12): 109918-. doi: 10.1016/j.cclet.2024.109918
11. [11]
  Dian-Xue Ma , Yu-Wu Zhong . Achieving highly-efficient room-temperature phosphorescence with a nylon matrix. Chinese Journal of Structural Chemistry, 2024, 43(9): 100391-100391. doi: 10.1016/j.cjsc.2024.100391
12. [12]
  Dan Ouyang , Huan Huang , Yanting He , Jiajing Chen , Jiali Lin , Zhuling Chen , Zongwei Cai , Zian Lin . Utilization of hydralazine as a reactive matrix for enhanced detection and on-MALDI-target derivatization of saccharides. Chinese Chemical Letters, 2024, 35(5): 108885-. doi: 10.1016/j.cclet.2023.108885
13. [13]
  Ran Wu , Dongxu Jiang , Hao Hu , Chenyu Yang , Liang Qin , Lulu Chen , Zehui Hu , Hualei Xu , Jinrong Li , Haiqiang Liu , Hua Guo , Jinxiang Fu , Qichen Hao , Yijun Zhou , Jinchao Feng , Qiang Wang , Xiaodong Wang . 4-Aminoazobenzene: A novel negative ion matrix for enhanced MALDI tissue imaging of metabolites. Chinese Chemical Letters, 2024, 35(11): 109624-. doi: 10.1016/j.cclet.2024.109624
14. [14]
  Haijun Shen , Yi Qiao , Chun Zhang , Yane Ma , Jialing Chen , Yingying Cao , Wenna Zheng . A matrix metalloproteinase-sensitive hydrogel combined with photothermal therapy for transdermal delivery of deferoxamine to accelerate diabetic pressure ulcer healing. Chinese Chemical Letters, 2024, 35(12): 110283-. doi: 10.1016/j.cclet.2024.110283
15. [15]
  Tianyi Yang , Fangxi Su , Dehuan Shi , Shenghong Zhong , Yalin Guo , Zhaohui Liu , Jianfeng Huang . Efficient propane dehydrogenation catalyzed by Ru nanoparticles anchored on a porous nitrogen-doped carbon matrix. Chinese Chemical Letters, 2025, 36(2): 110444-. doi: 10.1016/j.cclet.2024.110444
16. [16]
  Jiahao Xie , Jin Liu , Bin Liu , Xin Meng , Zhuang Cai , Xiaoqin Xu , Cheng Wang , Shijie You , Jinlong Zou . Yolk shell-structured pyrite-type cobalt sulfide grafted by nitrogen-doped carbon-needles with enhanced electrical conductivity for oxygen electrocatalysis. Chinese Chemical Letters, 2024, 35(7): 109236-. doi: 10.1016/j.cclet.2023.109236
17. [17]
  Hualei Xu , Manman Han , Haiqiang Liu , Liang Qin , Lulu Chen , Hao Hu , Ran Wu , Chenyu Yang , Hua Guo , Jinrong Li , Jinxiang Fu , Qichen Hao , Yijun Zhou , Jinchao Feng , Xiaodong Wang . 4-Nitrocatechol as a novel matrix for low-molecular-weight compounds in situ detection and imaging in biological tissues by MALDI-MSI. Chinese Chemical Letters, 2024, 35(6): 109095-. doi: 10.1016/j.cclet.2023.109095
18. [18]
  Pei Cao , Yilan Wang , Lejian Yu , Miao Wang , Liming Zhao , Xu Hou . Dynamic asymmetric mechanical responsive carbon nanotube fiber for ionic logic gate. Chinese Chemical Letters, 2024, 35(6): 109421-. doi: 10.1016/j.cclet.2023.109421
19. [19]
  Yihong Li , Zhong Qiu , Lei Huang , Shenghui Shen , Ping Liu , Haomiao Zhang , Feng Cao , Xinping He , Jun Zhang , Yang Xia , Xinqi Liang , Chen Wang , Wangjun Wan , Yongqi Zhang , Minghua Chen , Wenkui Zhang , Hui Huang , Yongping Gan , Xinhui Xia . Plasma enhanced reduction method for synthesis of reduced graphene oxide fiber/Si anode with improved performance. Chinese Chemical Letters, 2024, 35(11): 109510-. doi: 10.1016/j.cclet.2024.109510
20. [20]
  Manman Ou , Yunjian Zhu , Jiahao Liu , Zhaoxuan Liu , Jianjun Wang , Jun Sun , Chuanxiang Qin , Lixing Dai . Polyvinyl alcohol fiber with enhanced strength and modulus and intense cyan fluorescence based on covalently functionalized graphene quantum dots. Chinese Chemical Letters, 2025, 36(2): 110510-. doi: 10.1016/j.cclet.2024.110510

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(760)
HTML views(21)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142