Citation: Shan Zhi, Lyu Mingsheng, Curry Dennis, Oakley David, Oakes Ken, Zhang Xu. Cu-DNAzyme facilitates highly sensitive immunoassay[J]. Chinese Chemical Letters, ;2019, 30(9): 1652-1654. doi: 10.1016/j.cclet.2019.05.037 shu

Cu-DNAzyme facilitates highly sensitive immunoassay

Shan Zhi ^a,b ,
Lyu Mingsheng ^a,c ,
Curry Dennis ^a ,
Oakley David ^a ,
Oakes Ken ^d ,
Zhang Xu ^a,e,*,

a.
Verschuren Centre for Sustainability in Energy & the Environment, Cape Breton University, Sydney, Nova Scotia, B1P 6L2, Canada
b.
College of Life Science, Sichuan Agriculture University, Yaan 625014, China
c.
Jiangsu Key Laboratory of Marine Bioresources and Environment, Huaihai Institute of Technology, Lianyungang 222005, China
d.
Department of Biology, Cape Breton University, Sydney, Nova Scotia, B1P 6L2, Canada
e.
Department of Chemistry, Cape Breton University, Sydney, Nova Scotia, B1P 6L2, Canada

xu_zhang@cbu.ca

Received Date: 4 April 2019
Revised Date: 20 May 2019
Accepted Date: 20 May 2019
Available Online: 21 September 2019

Figures(4)

Fast and sensitive antigen detection is important in biomedical research and development. Despite, being invented 48 years ago, the enzyme-linked immunosorbent assay (ELISA) remains one of the most successful and widely employed bioanalytical techniques in research and clinical diagnostics due to its reliability and simplistic design. Recently, nanotechnology has offered efficient signal reporting. In spite of some improvements in these systems, there are typically increased material costs involved and the need for expensive equipment or complicated chemical processes, thus negating any possible benefits over ELISA. Herein, we communicate a simple Cu-DNAzyme system for signal transduction of a CuO nanoparticle-labeled immunoassay. The reported immunoassay amplifies signal generation similar to traditional ELISA and is fast, simple, cost-effective, and sensitive, holding promise for biomedical applications and point-of-care testing.
- Cu-DNAzyme,
- Immunoassay,
- ELISA,
- CuO,
- Nanoparticles
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