Citation: Sun Yanhui, Zhan Aiyan, Zhou Shuang, Kuang Xiao, Shen Hongyan, Liu Hongzhuo, Xu Youjun. A novel mitochondria-targeting tetrapeptide for subcellular delivery of nanoparticles[J]. Chinese Chemical Letters, ;2019, 30(7): 1435-1439. doi: 10.1016/j.cclet.2019.05.001 shu

A novel mitochondria-targeting tetrapeptide for subcellular delivery of nanoparticles

Sun Yanhui ^a ,
Zhan Aiyan ^b ,
Zhou Shuang ^b ,
Kuang Xiao ^b ,
Shen Hongyan ^a ,
Liu Hongzhuo ^b,*, ,
Xu Youjun ^a,*,

a.
School of Pharmaceutial Engineering, Key Laboratory of Structure-Based Drug Design & Discovery(Ministry of Education), Shenyang Pharmaceutical University, Shenyang 110016, China
b.
Wuya College of Innovation, Shenyang Pharmaceutical University, Shenyang 110016, China

liuhongzhuo@syphu.edu.cn

xuyoujun@syphu.edu.cn

Received Date: 16 March 2019
Revised Date: 19 April 2019
Accepted Date: 5 May 2019
Available Online: 7 July 2019

Figures(5)

Mitochondrial dysfunction is associated with the emergence of several neurological and cardiovascular diseases. Hence, mitochondria-targeting delivery strategies are highly significant and critically needed. In this study, we developed a small library of peptides simulating the mitochondria-targeting peptide SS-31, a promising tetra-peptide with antioxidant character, and subsequently evaluated the toxicity, antioxidant ability and mitochondrial delivery of nanoparticles. Among the designed peptides, RF-2 (DArg-Dmt-Arg-Phe-NH₂) showed controlled toxicity and excellent protection against gentamicin-induced hair cell damage, as compared with SS-31. More importantly, RF-2-modified PLGA nanoparticles demonstrated high colocalization with mitochondria and comparable specific subcellular accumulation, when compared with SS-31. Taken together, the obtained results supported RF-2 as a mitochondriatargeting peptide with high potential as a targeted carrier.
- Mitochondrial targeting,
- Peptide,
- SS-31,
- Toxicity,
- Nanoparticles,
- Zebrafish
1. [1]
  J. Zielonka, J. Joseph, A. Sikora, et al., Chem Rev. 117(2017) 10043-10120. doi: 10.1021/acs.chemrev.7b00042
2. [2]
  R.K. Pathak, N. Kolishetti, S. Dhar, Wiley Interdiscip. Rev. Nanomed. Nanobiotechnol. 7(2015) 315-329. doi: 10.1002/wnan.1305
3. [3]
  I. Pezzini, V. Mattoli, G. Ciofani, Expert. Opin. Drug Deliv. 14(2017) 513-523. doi: 10.1080/17425247.2016.1218461
4. [4]
  R. Esterberg, T. Linbo, S.B. Pickett, et al., J. Clin. Invest. 126(2016) 3556-3566. doi: 10.1172/JCI84939
5. [5]
  C.P. Ojano-Dirain, P.J. Antonelli, Laryngoscope 122(2012) 2543-2548.
6. [6]
  C.P. Ojano-Dirain, P.J. Antonelli, C.G. Le Prell, Otol. Neurotol. 35(2014) 533-539. doi: 10.1097/MAO.0000000000000192
7. [7]
  Z. Wang, X. Kuang, J. Shi, et al., Biomater. Sci. 5(2017) 1800-1809. doi: 10.1039/C7BM00224F
8. [8]
  S.E. Abu-Gosh, N. Kolvazon, B. Tirosh, et al., Mol. Pharm. 6(2009) 1138-1144. doi: 10.1021/mp900032r
9. [9]
  M.P. Murphy, Trends Biotechnol. 15(1997) 326-330. doi: 10.1016/S0167-7799(97)01068-8
10. [10]
  H.H. Szeto, A.V. Birk, Clin. Pharmacol. Ther. 96(2014) 672-683. doi: 10.1038/clpt.2014.174
11. [11]
  H.H. Szeto, Br. J. Pharmacol. 171(2014) 2029-2050. doi: 10.1111/bph.12461
12. [12]
  X. Kuang, S. Zhou, W. Guo, et al., Drug Deliv. 24(2017) 1750-1761. doi: 10.1080/10717544.2017.1402220
13. [13]
  P. Tveden-Nyborg, T.K. Bergmann, J. Lykkesfeldt, Basic Clin. Pharmacol. Toxicol. 123(2018) 233-235.
14. [14]
  C.P. Cerrato, M. Pirisinu, E.N. Vlachos, et al., FASEB J. 29(2015) 4589-4599. doi: 10.1096/fj.14-269225
15. [15]
  E. Rosini, L. Caldinelli, L. Piubelli, Front. Mol. Biosci. 4(2017) 102.
16. [16]
  Y.H. Chen, W.L. Chen, Y.H. Wang, et al., Fish Physiol Biochem. 33(2007) 73-80. doi: 10.1007/s10695-006-9118-1
17. [17]
  J. Sasabe, Y. Miyoshi, S. Rakoff-Nahoum, et al., Nat. Microbiol. 1(2016) 16125. doi: 10.1038/nmicrobiol.2016.125
18. [18]
  H. Ou, J.A. Simon, E.W. Rubel, et al., Hear. Res. 288(2012) 58-66. doi: 10.1016/j.heares.2012.01.009
19. [19]
  C. Bechara, S. Sagan, FEBS Lett. 587(2013) 1693-1702. doi: 10.1016/j.febslet.2013.04.031
20. [20]
  E.I. Geihe, C.B. Cooley, J.R. Simon, et al., Proc. Natl. Acad. Sci. U. S. A. 109(2012) 13171-13176. doi: 10.1073/pnas.1211361109
1. [1]
  Jianhui Yin , Wenjing Huang , Changyong Guo , Chao Liu , Fei Gao , Honggang Hu . Tryptophan-specific peptide modification through metal-free photoinduced N-H alkylation employing N-aryl glycines. Chinese Chemical Letters, 2024, 35(6): 109244-. doi: 10.1016/j.cclet.2023.109244
2. [2]
  Yuwen Zhu , Xiang Deng , Yan Wu , Baode Shen , Lingyu Hang , Yuye Xue , Hailong Yuan . Formation mechanism of herpetrione self-assembled nanoparticles based on pH-driven method. Chinese Chemical Letters, 2025, 36(1): 109733-. doi: 10.1016/j.cclet.2024.109733
3. [3]
  Bohan Chen , Liming Gong , Jing Feng , Mingji Jin , Liqing Chen , Zhonggao Gao , Wei Huang . Research advances of nanoparticles for CAR-T therapy in solid tumors. Chinese Chemical Letters, 2024, 35(9): 109432-. doi: 10.1016/j.cclet.2023.109432
4. [4]
  Wei Su , Xiaoyan Luo , Peiyuan Li , Ying Zhang , Chenxiang Lin , Kang Wang , Jianzhuang Jiang . Phthalocyanine self-assembled nanoparticles for type Ⅰ photodynamic antibacterial therapy. Chinese Chemical Letters, 2024, 35(12): 109522-. doi: 10.1016/j.cclet.2024.109522
5. [5]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
6. [6]
  Deshuai Zhen , Chunlin Liu , Qiuhui Deng , Shaoqi Zhang , Ningman Yuan , Le Li , Yu Liu . A review of covalent organic frameworks for metal ion fluorescence sensing. Chinese Chemical Letters, 2024, 35(8): 109249-. doi: 10.1016/j.cclet.2023.109249
7. [7]
  Yihao Zhang , Yang Jiao , Xianchao Jia , Qiaojia Guo , Chunying Duan . Highly effective self-assembled porphyrin MOCs nanomaterials for enhanced photodynamic therapy in tumor. Chinese Chemical Letters, 2024, 35(5): 108748-. doi: 10.1016/j.cclet.2023.108748
8. [8]
  Yixin Zhang , Ting Wang , Jixiang Zhang , Pengyu Lu , Neng Shi , Liqiang Zhang , Weiran Zhu , Nongyue He . Formation mechanism for stable system of nanoparticle/protein corona and phospholipid membrane. Chinese Chemical Letters, 2024, 35(4): 108619-. doi: 10.1016/j.cclet.2023.108619
9. [9]
  Keyang Li , Yanan Wang , Yatao Xu , Guohua Shi , Sixian Wei , Xue Zhang , Baomei Zhang , Qiang Jia , Huanhua Xu , Liangmin Yu , Jun Wu , Zhiyu He . Flash nanocomplexation (FNC): A new microvolume mixing method for nanomedicine formulation. Chinese Chemical Letters, 2024, 35(10): 109511-. doi: 10.1016/j.cclet.2024.109511
10. [10]
  Yujie Li , Ya-Nan Wang , Yin-Gen Luo , Hongcai Yang , Jinrui Ren , Xiao Li . Advances in synthetic biology-based drug delivery systems for disease treatment. Chinese Chemical Letters, 2024, 35(11): 109576-. doi: 10.1016/j.cclet.2024.109576
11. [11]
  Fereshte Hassanzadeh-Afruzi , Mina Azizi , Iman Zare , Ehsan Nazarzadeh Zare , Anwarul Hasan , Siavash Iravani , Pooyan Makvandi , Yi Xu . Advanced metal-organic frameworks-polymer platforms for accelerated dermal wound healing. Chinese Chemical Letters, 2024, 35(11): 109564-. doi: 10.1016/j.cclet.2024.109564
12. [12]
  Shenglan Zhou , Haijian Li , Hongyi Gao , Ang Li , Tian Li , Shanshan Cheng , Jingjing Wang , Jitti Kasemchainan , Jianhua Yi , Fengqi Zhao , Wengang Qu . Recent advances in metal-loaded MOFs photocatalysts: From single atom, cluster to nanoparticle. Chinese Chemical Letters, 2025, 36(1): 110142-. doi: 10.1016/j.cclet.2024.110142
13. [13]
  Yixuan Zhu , Qingtong Wang , Jin Li , Lin Chen , Junlong Zhao . Blog of Oxytocin. University Chemistry, 2024, 39(9): 134-140. doi: 10.12461/PKU.DXHX202310090
14. [14]
  Yue WANG , Zhizhi GU , Jingyi DONG , Jie ZHU , Cunguang LIU , Guohan LI , Meichen LU , Jian HAN , Shengnan CAO , Wei WANG . Effects of kelp-derived carbon dots on embryonic development of zebrafish. Chinese Journal of Inorganic Chemistry, 2024, 40(6): 1209-1217. doi: 10.11862/CJIC.20230423
15. [15]
  Weiyu Chen , Zenghui Li , Chenguang Zhao , Lisha Zha , Junfeng Shi , Dan Yuan . Enzyme-modulate conformational changes in amphiphile peptide for selectively cell delivery. Chinese Chemical Letters, 2024, 35(12): 109628-. doi: 10.1016/j.cclet.2024.109628
16. [16]
  Xiaofang Luo , Ye Wu , Xiaokun Zhang , Min Tang , Feiye Ju , Zuodong Qin , Gregory J Duns , Wei-Dong Zhang , Jiang-Jiang Qin , Xin Luan . Peptide-based strategies for overcoming multidrug-resistance in cancer therapy. Chinese Chemical Letters, 2025, 36(1): 109724-. doi: 10.1016/j.cclet.2024.109724
17. [17]
  Pengyu Chen , Beibei Chen , Man He , Yuxi Zhou , Lei Lei , Jian Han , Bingsheng Zhou , Ligang Hu , Bin Hu . Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging. Chinese Chemical Letters, 2025, 36(2): 109908-. doi: 10.1016/j.cclet.2024.109908
18. [18]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
19. [19]
  Cheng-Yan Wu , Yi-Nan Gao , Zi-Han Zhang , Rui Liu , Quan Tang , Zhong-Lin Lu . Enhancing self-assembly efficiency of macrocyclic compound into nanotubes by introducing double peptide linkages. Chinese Chemical Letters, 2024, 35(11): 109649-. doi: 10.1016/j.cclet.2024.109649
20. [20]
  Bharathi Natarajan , Palanisamy Kannan , Longhua Guo . Metallic nanoparticles for visual sensing: Design, mechanism, and application. Chinese Journal of Structural Chemistry, 2024, 43(9): 100349-100349. doi: 10.1016/j.cjsc.2024.100349

Get Citation

PDF

XML

Metrics

PDF Downloads(19)
Abstract views(1033)
HTML views(150)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142