Citation: Ding Cong, Chen Yage, Li Haozheng, Wang Bingyao, Wei Qi, Tang Huajun, Jia Shaokang, He Zhiyong, Wang Ping, Zhou Xiang. Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne[J]. Chinese Chemical Letters, ;2019, 30(7): 1393-1396. doi: 10.1016/j.cclet.2019.03.046 shu

Photostable lysosomal imaging of living cell with hyperspectral stimulated Raman scattering microscopy using a probe based on bisarylbutadiyne

Ding Cong ^a,1 ,
Chen Yage ^b,c,1 ,
Li Haozheng ^b,c,1 ,
Wang Bingyao ^a ,
Wei Qi ^a ,
Tang Huajun ^b,c ,
Jia Shaokang ^a ,
He Zhiyong ^a,*, ,
Wang Ping ^b,c ,
Zhou Xiang ^a,*,

a.
College of Chemistry and Molecular Sciences, Key Laboratory of Biomedical Polymers of the Ministry of Education, WuhanUniversity, Wuhan 430072, China
b.
Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology, Wuhan 430074, China
c.
MoE Key Laboratory for Biomedical Photonics, Collaborative Innovation Center for Biomedical Engineering, School of Engineering Sciences, Huazhong University of Science and Technology, Wuhan 430074, China

hezy@whu.edu.cn

xzhou@whu.edu.cn

Received Date: 26 February 2019
Revised Date: 25 March 2019
Accepted Date: 26 March 2019
Available Online: 27 July 2019

Figures(4)

We designed a lysosome-selective Raman probe by conjugating bisphenylbutadiyne with morpholine, a well-known lysosome targeting moiety. This probe, named Lyso-BADY, has a Raman peak 28 times more intense than that of 5-ethynyl-2'-deoxyuridine. Lysosome in living cells was successfully visualized by hyperspectral stimulated Raman scattering (SRS) microscopy with this extracellular probe. Further study showed that the Raman signal of Lyso-BADY remained steady and strong even after a prolonged irradiation time. The photo-stability feature of Lyso-BADY rendered molecules of the similar structure as potentially versatile probe for continuous imaging in the future
- Raman probe,
- SRS,
- Cell imaging,
- Photo-stability feature
1. [1]
  L. Wei, Z. Chen, L. Shi, et al., Nature 544(2017) 465-470. doi: 10.1038/nature22051
2. [2]
  L. Wei, F. Hu, Y. Shen, et al., Nat. Methods 11(2014) 410-412. doi: 10.1038/nmeth.2878
3. [3]
  Z. Zhao, Y. Shen, F. Hu, W. Min, Analyst 142(2017) 4018-4029. doi: 10.1039/C7AN01001J
4. [4]
  C.W. Freudiger, W. Min, B.G. Saar, et al., Science 322(2008) 1857-1861. doi: 10.1126/science.1165758
5. [5]
  H. Lei, Y. Hu, G. Li, Chin. Chem. Lett. 29(2018) 509-512. doi: 10.1016/j.cclet.2017.08.012
6. [6]
  H. Zhang, L. Sun, Y. Zhang, et al., Chin. Chem. Lett. 29(2018) 981-984. doi: 10.1016/j.cclet.2017.10.017
7. [7]
  F. Hu, C. Zeng, R. Long, et al., Nat. Methods 15(2018) 194-200. doi: 10.1038/nmeth.4578
8. [8]
  L. Wei, F. Hu, Z. Chen, Acc. Chem. Res. 49(2016) 1494-1502. doi: 10.1021/acs.accounts.6b00210
9. [9]
  Z. Chen, D.W. Paley, L. Wei, et al., J. Am. Chem. Soc. 136(2014) 8027-8033. doi: 10.1021/ja502706q
10. [10]
  Z.L. Song, Z. Chen, X. Bian, et al., J. Am. Chem. Soc. 136(2014) 13558-13561. doi: 10.1021/ja507368z
11. [11]
  L. Wei, Y. Shen, F. Xu, et al., ACS Chem. Biol. 10(2015) 901-908. doi: 10.1021/cb500787b
12. [12]
  H. Yamakoshi, K. Dodo, M. Okada, et al., J. Am. Chem. Soc. 133(2011) 6102-6105. doi: 10.1021/ja108404p
13. [13]
  F. Hu, Z. Chen, L. Zhang, et al., Angew. Chem. Int. Ed. 54(2015) 9821-9825. doi: 10.1002/anie.201502543
14. [14]
  L. Wei, Y. Yu, Y. Shen, M.C. Wang, W. Min, Proc. Natl. Acad. Sci. U. S. A.110(2013) 11226-11231. doi: 10.1073/pnas.1303768110
15. [15]
  L. Lin, X. Tian, S. Hong, Angew. Chem. Int. Ed. 52(2013) 7266-7271. doi: 10.1002/anie.201301387
16. [16]
  Y. Shen, Z. Zhao, L. Zhang, Proc. Natl. Acad. Sci. U. S. A.114(2017) 13394-13399. doi: 10.1073/pnas.1712555114
17. [17]
  A.C. Johansson, H. Appelqvist, C. Nilsson, et al., Apoptosis 15(2010) 527-540. doi: 10.1007/s10495-009-0452-5
18. [18]
  C. Settembre, A. Fraldi, D.L. Medina, A. Ballabio, Nature Rev. Mol. Cell Biol. 14(2013) 283-296. doi: 10.1038/nrm3565
19. [19]
  H. Xu, D. Ren, Annu. Rev. Physiol. 77(2015) 57-80. doi: 10.1146/annurev-physiol-021014-071649
20. [20]
  F.M. Platt, B. Boland, A.C. van der Spoel, J. Cell Biol. 199(2012) 723-734. doi: 10.1083/jcb.201208152
21. [21]
  B.L. Wei, P.W. Denton, E. O'Neill, et al., J. Virol. 79(2005) 5705-5712. doi: 10.1128/JVI.79.9.5705-5712.2005
22. [22]
  X.J. Cao, L.N. Chen, X. Zhang, et al., Anal. Chim. Acta 920(2016) 86-93. doi: 10.1016/j.aca.2016.03.029
23. [23]
  M. Li, H. Ge, V. Mirabello, et al., Chem. Commun. (Camb.) 53(2017) 11161-11164. doi: 10.1039/C7CC05166B
24. [24]
  X.L. Shi, G.J. Mao, X.B. Zhang, et al., Talanta 130(2014) 356-362. doi: 10.1016/j.talanta.2014.07.030
25. [25]
  X. Zhou, F. Su, H. Lu, et al., Biomaterials 33(2012) 171-180. doi: 10.1016/j.biomaterials.2011.09.053
26. [26]
  J.A. Mindell, Annu. Rev. Physiol. 74(2012) 69-86. doi: 10.1146/annurev-physiol-012110-142317
27. [27]
  H. Yamakoshi, K. Dodo, A. Palonpon, et al., J. Am. Chem. Soc. 134(2012) 20681-20689. doi: 10.1021/ja308529n
28. [28]
  J. Zhang, S. Yan, Z. He, et al., J. Phys. Chem. Lett. 9(2018) 4679-4685. doi: 10.1021/acs.jpclett.8b01991
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