Citation: Chen Jiancui, Xue Huifeng, Chen Qiaoyun, Lin Yao, Tang Dianping, Zheng Jinwen. Enzyme-conjugated hybridization chain reaction for magneto-controlled immunoassay of squamous cell carcinoma antigen with pH meter[J]. Chinese Chemical Letters, ;2019, 30(9): 1631-1634. doi: 10.1016/j.cclet.2019.03.045 shu

Enzyme-conjugated hybridization chain reaction for magneto-controlled immunoassay of squamous cell carcinoma antigen with pH meter

a.
Cervical Disease Diagnosis and Treatment Health Center, Fujian Provincial Maternity and Children's Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou 350001, China
b.
College of Life Sciences, Fujian Normal University, Fuzhou 350117, China
c.
Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), Department of Chemistry, Fuzhou University, Fuzhou 350116, China

dianping.tang@fzu.edu.cn

3442733394@qq.com

Received Date: 1 March 2019
Revised Date: 25 March 2019
Accepted Date: 26 March 2019
Available Online: 30 September 2019

Figures(4)

A nanoparticle-based potentiometric immunoassay was designed for the sensitive detection of squamous cell carcinoma antigen (SCCA; cervical carcinoma marker) on a portable pH meter coupling enzyme-labeled hybridization chain reaction (HCR) with two alternating hairpin DNA probes for the signal amplification. Initially, a sandwich-type immunoreaction was carried out between anti-SCCA capture antibody-conjugated magnetic bead and detection antibody/initiator strand-coated gold nanoparticle (AuNP). Then, the HCR reaction was readily executed between two glucose oxidase (GOx)-labeled hairpins through the initiator strand to form numerous GOx concatamers on the AuNP via the long nicked double-helix. The concatenated GOx oxidized glucose into gluconic acid and hydrogen peroxide, thus resulting in the pH change of the detection solution on a handheld pH meter. Several labeling protocols including GOx-antibody, GOx-AuNP-antibody and GOx-HCR-AuNP-antibody were investigated for detection of target SCCA, and improved analytical features were obtained with the immune-HCR assay. Under optimum conditions, the immune-HCR assay exhibited good pH responses for the determination of SCCA at a concentration as low as 5.7 pg/mL. Additionally, the immune-HCR assay had good precision and reproducibility, high specificity, and acceptable accuracy for analyzing human serum specimens.
- Immune-HCR assay,
- pH Meter,
- Molecular biological amplification,
- Nano label,
- Enzyme label
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1.
沈阳化工大学材料科学与工程学院沈阳 110142