Citation: Lu Zhuoxuan, Xu Lingfeng, He Nongyue, Huang Fengying, Xu Tiefeng, Li Li, Zhang Yanwei, Zhang Liming. Cy5.5-MSA-G250 nanoparticles (CMGNPs) induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner[J]. Chinese Chemical Letters, ;2019, 30(6): 1320-1324. doi: 10.1016/j.cclet.2019.03.001 shu

Cy5.5-MSA-G250 nanoparticles (CMGNPs) induce M1 polarity of RAW264. 7 macrophage cells via TLR4-dependent manner

1.
Key Laboratory of Tropical Disease and Translational Medicine of the Ministry of Education & Hainan Provincial Key Laboratory of Tropical Medicine, Hainan Medical College, Haikou 571101, China
2.
Hunan Key Laboratory of Biomedical Materials and Devices, Hunan University of Technology, Zhuzhou 412008, China
3.
The First Affiliated Hospital and the Oncological Institute of Hainan Medical College, Haikou 571101, China

lmzhang1980@163.com

Received Date: 15 January 2019
Revised Date: 26 February 2019
Accepted Date: 1 March 2019
Available Online: 1 June 2019

Figures(4)

Cy5.5-MSA-G250 nanoparticles (CMGNPs) had been proved to have unique advantages for cancer treatment, including excellent photothermal performance, tumor cell-selective cytotoxicity, direct visualization, and good biocompatibility. However, to cellular systems, the CMGNPs are considered as foreign invaders, and the effect of CMGNPs on immunity system is still unknown. Therefore, more efforts are needed to understand the role of CMGNPs on the immunity system. In this study, we attempted to screen the pro-inflammatory responses on RAW264.7 macrophages after treated with the CMGNPs. In vitro experiments clearly showed that CMGNPs not only enhances phagocytosis capacity of RAW264.7 cells, but also promotes M1 polarization, associated with changes in cell morphology and increased expression of inflammatory cytokines. This ability to induce M1 polarization may be beneficial to CMGNPs to achieve better anticancer effects in clinical trials. Moreover, the observed M1 macrophages' polarization triggered by CMGNPs can be abolished after adding TLR4 inhibitor, CLI095, suggesting that TLR4 is involved in CMGNP-induced inflammation.
- CMGNPs,
- Macrophage,
- Polarization,
- Pro-inflammatory cytokines,
- TLR 4 pathway
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1.
沈阳化工大学材料科学与工程学院沈阳 110142