Citation: Cheng Guorong, Xing Junpeng, Pi Zifeng, Liu Shu, Liu Zhiqiang, Song Fengrui. α-Glucosidase immobilization on functionalized Fe₃O₄ magnetic nanoparticles for screening of enzyme inhibitors[J]. Chinese Chemical Letters, ;2019, 30(3): 656-659. doi: 10.1016/j.cclet.2018.12.003 shu

α-Glucosidase immobilization on functionalized Fe₃O₄ magnetic nanoparticles for screening of enzyme inhibitors

Cheng Guorong ^a,b ,
Xing Junpeng ^a ,
Pi Zifeng ^a ,
Liu Shu ^a,*, ,
Liu Zhiqiang ^a ,
Song Fengrui ^a,*,

a.
National Center of Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
University of Science and Technology of China, Hefei 230029, China

mslab20@ciac.ac.cn

songfr@ciac.ac.cn

Received Date: 28 September 2018
Revised Date: 30 November 2018
Accepted Date: 3 November 2018
Available Online: 4 March 2018

Figures(2)

Magnetic nanoparticles (MNPs) are widely used for the immobilization of enzyme owing to the unique properties such as good biocompatibility and rapid separation. Herein, we used Fe₃O₄ magnetic nanoparticles (Fe₃O₄ MNPs) as the carrier core with (3-aminopropyl)triethoxysilane (APTES) modification by our approach, in which α-glucosidase was stereoscopically immobilized on the surface of Fe₃O₄ MNPs via covalent binding. The result of immobilization was characterized by scanning electron microscope (SEM) and fourier transform-infrared spectroscopy (FT-IR). Then we optimized some key parameters of the immobilization reaction, including the ratio of MNPs to enzyme, GA concentration, crosslinking time and immobilization time. Moreover, under the optimal conditions, pH tolerance, thermo stability and reusability of the immobilized enzyme were investigated and compared with the free one. In order to evaluate the change of the affinity of the enzyme to its specific substrate after immobilization, the Michaelis-Menten constant (K_m) was also studied. Finally, the immobilized α-glucosidase combining with high performance liquid chromatography-tandem mass spectrometry technique (HPLC-MS/MS) was applied to screen and identify eight inhibitors from Polygonum cuspidatum extract. These results indicated that the established method had the broad prospects for biotechnological applications.
- Magnetic nanoparticles (MNPs),
- α-Glucosidase,
- Immobilization,
- HPLC-MS/MS,
- Inhibitor screening,
- Polygonum cuspidatum
1. [1]
  H. Gao, Y.N. Huang, B. Gao, et al., Food Chem. 106(2008) 1195-1201. doi: 10.1016/j.foodchem.2007.07.064
2. [2]
  J.H. O'Keefe, D.S.H. Bell, Am. J. Cardiol. 100(2007) 899-904. doi: 10.1016/j.amjcard.2007.03.107
3. [3]
  C. Schnack, G. Röggla, A. Luger, G. Schernthaner, Eur. J. Clin. Pharmacol. 30(1986) 417-419. doi: 10.1007/BF00607953
4. [4]
  M. Hanefeld, R.G. Josse, J.L. Chiasson, Diabetes Care 28(2005) 1840-1841. doi: 10.2337/diacare.28.7.1840
5. [5]
  J.A. Balfour, D. McTavish, Drugs 46(1993) 1025-1054. doi: 10.2165/00003495-199346060-00007
6. [6]
  J.P. Sels, M.S. Huijberts, B.H. Wolffenbuttel, Expert Opin. Pharmacother. 1(1999) 149-156. doi: 10.1517/14656566.1.1.149
7. [7]
  D.M. Liu, J. Chen, Y.P. Shi, Talanta 164(2017) 548-555. doi: 10.1016/j.talanta.2016.12.028
8. [8]
  D. Tang, J.X. Zhu, A.G. Wu, et al., J. Chromatogr. A 1286(2013) 102-110. doi: 10.1016/j.chroma.2013.02.058
9. [9]
  W.J. Chen, Y. Wu, X. Zhao, et al., Chin. Chem. Lett. 27(2016) 1701-1707. doi: 10.1016/j.cclet.2016.05.022
10. [10]
  Y. Zhao, M.X. Chen, K.T. Kongstad, A.K. Jager, D. Staerk, J. Agric. Food Chem. 65(2017) 4421-4427. doi: 10.1021/acs.jafc.7b01353
11. [11]
  Z. Pan, H. Liang, C. Liang, W. Xu, Chin. J. Chromatogr. 33(2015) 22-28. doi: 10.3724/SP.J.1123.2014.07006
12. [12]
  F. Li, Z. Zhan, F. Liu, et al., Org. Lett. 15(2013) 674-677. doi: 10.1021/ol3035033
13. [13]
  Z. Uddin, Y.H. Song, M.J. Curtis-Long, et al., J. Ethnopharmacol. 193(2016) 283-292. doi: 10.1016/j.jep.2016.08.026
14. [14]
  E. Chaita, E. Gikas, N. Aligiannis, Phytochem. Anal. 28(2017) 125-131. doi: 10.1002/pca.v28.2
15. [15]
  Y.T. Zhu, X.Y. Ren, L. Yuan, et al., Food Chem. 173(2015) 521-526. doi: 10.1016/j.foodchem.2014.10.087
16. [16]
  E.M. Forsberg, J.R. Green, J.D. Brennan, Anal. Chem. 83(2011) 5230-5236. doi: 10.1021/ac200534t
17. [17]
  J. Dong, W. Ning, W. Liu, M.L. Bruening, Analyst 142(2017) 2578-2586. doi: 10.1039/C7AN00778G
18. [18]
  S. Talekar, A. Joshi, S. Kambale, et al., Chem. Eng. J. 325(2017) 80-90. doi: 10.1016/j.cej.2017.05.054
19. [19]
  Y. Rui, X.M. Wu, B.D. Ma, Y. Xu, Chin. Chem. Lett. 29(2018) 1387-1390. doi: 10.1016/j.cclet.2017.10.033
20. [20]
  M. Asgher, S. Noreen, M. Bilal, Int. J. Biol. Macromol. 95(2017) 54-62. doi: 10.1016/j.ijbiomac.2016.11.012
21. [21]
  Z. Fathi, E. Doustkhah, S. Rostamnia, et al., Int. J. Biol. Macromol. 117(2018) 218-224. doi: 10.1016/j.ijbiomac.2018.05.156
22. [22]
  D.M. Liu, J. Chen, Y.P. Shi, Int. J. Biol. Macromol. 105(2017) 308-316. doi: 10.1016/j.ijbiomac.2017.07.045
23. [23]
  W. Feng, J. Qiao, J. Jiang, et al., Talanta 182(2018) 600-605. doi: 10.1016/j.talanta.2018.02.029
24. [24]
  M.R. Ladole, J.S. Mevada, A.B. Pandit, Bioresource Technol. 239(2017) 117-126. doi: 10.1016/j.biortech.2017.04.096
25. [25]
  T. Wang, D. Li, B. Yu, J. Qi, J. Sep. Sci. 40(2017) 1877-1886. doi: 10.1002/jssc.v40.9
26. [26]
  A. Soozanipour, A. Taheri-Kafrani, A. Landarani Isfahani, Chem. Eng. J. 270(2015) 235-243. doi: 10.1016/j.cej.2015.02.032
27. [27]
  X.C. Li, X. Fan, L.J. Han, et al., Chin. J. Mar. Drugs 21(2002) 8-11.
28. [28]
  P. Sledz, S. Lang, C.J. Stubbs, C. Abell, Angew. Chem. Int. Ed. 51(2012) 7680-7683. doi: 10.1002/anie.201202660
29. [29]
  M. Wang, Z. Xu, L. Chen, H. Yin, S. Ai, Anal. Chem. 84(2012) 9072-9078. doi: 10.1021/ac301620m
30. [30]
  T. Matsui, T. Ueda, T. Oki, et al., J. Agric. Food Chem. 49(2001) 1948-1951. doi: 10.1021/jf001251u
31. [31]
  Y. Tao, Y. Zhang, Y. Wang, Y. Cheng, Anal. Chim. Acta 785(2013) 75-81. doi: 10.1016/j.aca.2013.04.058
32. [32]
  Y. Tao, Z. Chen, Y. Zhang, Y. Wang, Y. Cheng, J. Pharm. Biomed. Anal. 78-79(2013) 190-201.
33. [33]
  J. Cao, J.J. Xu, X.G. Liu, S.L. Wang, L.Q. Peng, J. Chromatogr. A 1468(2016) 86-94. doi: 10.1016/j.chroma.2016.09.022
34. [34]
  X. Wang, L. Wang, X. He, Y. Zhang, L. Chen, Talanta 78(2009) 327-332. doi: 10.1016/j.talanta.2008.11.024
35. [35]
  S. Zhang, D. Wu, H. Li, et al., Food Funct. 8(2017) 3219-3227. doi: 10.1039/C7FO00928C
36. [36]
  W. Hu, Z. Lou, Chem. Res. 24(2013) 144-148.
37. [37]
  J.C. Feng, S.R. Yu, J. Li, T. Mo, P. Li, Chem. Eng. J. 286(2016) 216-222. doi: 10.1016/j.cej.2015.10.083
38. [38]
  D.M. Liu, J. Chen, Y.P. Shi, Anal. Chim. Acta 1006(2018) 90-98. doi: 10.1016/j.aca.2017.12.022
39. [39]
  D.M. Liu, J. Chen, Y.P. Shi, RSC Adv. 5(2015) 56841-56847. doi: 10.1039/C5RA07982A
40. [40]
  T. Li, S. Li, N. Wang, L. Tain, Food Chem. 109(2008) 703-708. doi: 10.1016/j.foodchem.2008.01.012
1. [1]
  Jia Chen , Yun Liu , Zerong Long , Yan Li , Hongdeng Qiu . Colorimetric detection of α-glucosidase activity using Ni-CeO₂ nanorods and its application to potential natural inhibitor screening. Chinese Chemical Letters, 2024, 35(9): 109463-. doi: 10.1016/j.cclet.2023.109463
2. [2]
  Yiran Tao , Chunlei Dai , Zhaoxiang Xie , Xinru You , Kaiwen Li , Jun Wu , Hai Huang . Redox responsive polymeric nanoparticles enhance the efficacy of cyclin dependent kinase 7 inhibitor for enhanced treatment of prostate cancer. Chinese Chemical Letters, 2024, 35(8): 109170-. doi: 10.1016/j.cclet.2023.109170
3. [3]
  Jisheng Liu , Junli Chen , Xifeng Zhang , Yin Wu , Xin Qi , Jie Wang , Xiang Gao . Red blood cell membrane-coated FLT3 inhibitor nanoparticles to enhance FLT3-ITD acute myeloid leukemia treatment. Chinese Chemical Letters, 2024, 35(9): 109779-. doi: 10.1016/j.cclet.2024.109779
4. [4]
  Guo-Ping Yin , Ya-Juan Li , Li Zhang , Ling-Gao Zeng , Xue-Mei Liu , Chang-Hua Hu . Citrinsorbicillin A, a novel homotrimeric sorbicillinoid isolated by LC-MS-guided with cytotoxic activity from the fungus Trichoderma citrinoviride HT-9. Chinese Chemical Letters, 2024, 35(8): 109035-. doi: 10.1016/j.cclet.2023.109035
5. [5]
  Pengyu Chen , Beibei Chen , Man He , Yuxi Zhou , Lei Lei , Jian Han , Bingsheng Zhou , Ligang Hu , Bin Hu . Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging. Chinese Chemical Letters, 2025, 36(2): 109908-. doi: 10.1016/j.cclet.2024.109908
6. [6]
  Wantong Zhang , Zixing Xu , Guofei Dai , Zhijian Li , Chunhui Deng . Removal of Microcystin-LR in lake water sample by hydrophilic mesoporous silica composites under high-throughput MALDI-TOF MS detection platform. Chinese Chemical Letters, 2024, 35(5): 109135-. doi: 10.1016/j.cclet.2023.109135
7. [7]
  Jun-Jie Fang , Zheng Liu , Yun-Peng Xie , Xing Lu . Superatomic Ag₅₈ nanoclusters incorporating a [MS₄@Ag₁₂]²⁺ (M = Mo or W) kernel show aggregation-induced emission. Chinese Chemical Letters, 2024, 35(10): 109345-. doi: 10.1016/j.cclet.2023.109345
8. [8]
  Zian Lin , Yingxue Jin . Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI-MS) for Disease Marker Screening and Identification: A Comprehensive Experiment Teaching Reform in Instrumental Analysis. University Chemistry, 2024, 39(11): 327-334. doi: 10.12461/PKU.DXHX202403066
9. [9]
  Yong-Fang Shi , Sheng-Hua Zhou , Zuju Ma , Xin-Tao Wu , Hua Lin , Qi-Long Zhu . From [Ba3S][GeS4] to [Ba3CO3][MS4] (M = Ge, Sn): Enhancing optical anisotropy in IR birefringent crystals via functional group implantation. Chinese Journal of Structural Chemistry, 2025, 44(1): 100455-100455. doi: 10.1016/j.cjsc.2024.100455
10. [10]
  Hongmei Yu , Baoxi Zhang , Meiju Liu , Cheng Xing , Guorong He , Li Zhang , Ningbo Gong , Yang Lu , Guanhua Du . Theoretical and experimental cocrystal screening of temozolomide with a series of phenolic acids, promising cocrystal coformers. Chinese Chemical Letters, 2024, 35(5): 109032-. doi: 10.1016/j.cclet.2023.109032
11. [11]
  Beitong Zhu , Xiaorui Yang , Lirong Jiang , Tianhong Chen , Shuangfei Wang , Lintao Zeng . A portable and versatile fluorescent platform for high-throughput screening of toxic phosgene, diethyl chlorophosphate and volatile acyl chlorides. Chinese Chemical Letters, 2025, 36(1): 110222-. doi: 10.1016/j.cclet.2024.110222
12. [12]
  Qingyun Hu , Wei Wang , Junyuan Lu , He Zhu , Qi Liu , Yang Ren , Hong Wang , Jian Hui . High-throughput screening of high energy density LiMn_1-xFe_xPO₄ via active learning. Chinese Chemical Letters, 2025, 36(2): 110344-. doi: 10.1016/j.cclet.2024.110344
13. [13]
  Chao Chen , Wenwen Yu , Guangen Huang , Xuelian Ren , Xiangli Chen , Yixin Li , Shenggui Liang , Mengmeng Xu , Mingyue Zheng , Yaxi Yang , He Huang , Wei Tang , Bing Zhou . Asymmetric macrocyclization enabled by Rh(Ⅲ)-catalyzed CH activation: Enantioenriched macrocyclic inhibitor of Zika virus infection. Chinese Chemical Letters, 2024, 35(11): 109574-. doi: 10.1016/j.cclet.2024.109574
14. [14]
  Shuheng Zhang , Yuanyuan Zhang , Wanyu Wang , Yuzhu Hu , Xinchuan Chen , Bilan Wang , Xiang Gao . A combination strategy of DOX and VEGFR-2 targeted inhibitor based on nanomicelle for enhancing lymphoma therapy. Chinese Chemical Letters, 2024, 35(12): 109658-. doi: 10.1016/j.cclet.2024.109658
15. [15]
  Bharathi Natarajan , Palanisamy Kannan , Longhua Guo . Metallic nanoparticles for visual sensing: Design, mechanism, and application. Chinese Journal of Structural Chemistry, 2024, 43(9): 100349-100349. doi: 10.1016/j.cjsc.2024.100349
16. [16]
  Hongdao LI , Shengjian ZHANG , Hongmei DONG . Magnetic relaxation and luminescent behavior in nitronyl nitroxide-based annuluses of rare-earth ions. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 972-978. doi: 10.11862/CJIC.20230411
17. [17]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
18. [18]
  Mengyuan Li , Xitong Ren , Yanmei Gao , Mengyao Mu , Shiping Zhu , Shufang Tian , Minghua Lu . Constructing bifunctional magnetic porous poly(divinylbenzene) polymer for high-efficient removal and sensitive detection of bisphenols. Chinese Chemical Letters, 2024, 35(12): 109699-. doi: 10.1016/j.cclet.2024.109699
19. [19]
  Zhao-Bo Hu , Ling-Ao Gui , Long-He Li , Tong-Tong Xiao , Adam T. Hand , Pagnareach Tin , Mykhaylo Ozerov , Yan Peng , Zhongwen Ouyang , Zhenxing Wang , Zi-Ling Xue , You Song . Co^Ⅱ single-ion magnet and its multi-dimensional aggregations: Influence of the structural rigidity on magnetic relaxation process. Chinese Chemical Letters, 2025, 36(2): 109600-. doi: 10.1016/j.cclet.2024.109600
20. [20]
  Xiaoling WANG , Hongwu ZHANG , Daofu LIU . Synthesis, structure, and magnetic property of a cobalt(Ⅱ) complex based on pyridyl-substituted imino nitroxide radical. Chinese Journal of Inorganic Chemistry, 2025, 41(2): 407-412. doi: 10.11862/CJIC.20240214

Get Citation

PDF

XML

Metrics

PDF Downloads(9)
Abstract views(789)
HTML views(74)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

α-Glucosidase immobilization on functionalized Fe3O4 magnetic nanoparticles for screening of enzyme inhibitors

Metrics

通讯作者: 陈斌, bchen63@163.com

α-Glucosidase immobilization on functionalized Fe₃O₄ magnetic nanoparticles for screening of enzyme inhibitors