Citation: Wang Zhenyu, Wu Chaoxiong, Fan Ting, Han Xianwei, Wang Qiong, Lei Jincan, Yang Jun. Electroformation and collection of giant liposomes on an integrated microchip[J]. Chinese Chemical Letters, ;2019, 30(2): 353-358. doi: 10.1016/j.cclet.2018.12.001 shu

Electroformation and collection of giant liposomes on an integrated microchip

Wang Zhenyu ^a,b,*, ,
Wu Chaoxiong ^a ,
Fan Ting ^b ,
Han Xianwei ^b ,
Wang Qiong ^b,c ,
Lei Jincan ^c ,
Yang Jun ^b,*,

a.
State Key Laboratory of Ultrasound Engineering in Medicine Co-Founded by Chongqing & the Ministry of Science & Technology, College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
b.
Key Laboratory of Biorheological Science and Technology (Chongqing University), Ministry of Education, and Key Lab of Visual Damage and Regeneration & Restoration of Chongqing, Bioengineering College, Chongqing University, Chongqing 400030, China
c.
Chongqing Engineering and Technology Research Center of Intelligent Rehabilitation and Eldercare, Chongqing City Management College, Chongqing 401331, China

gdwzycq@163.com

bioyangjun@cqu.edu.cn

Received Date: 5 September 2018
Revised Date: 29 November 2018
Accepted Date: 3 December 2018
Available Online: 3 February 2018

Figures(5)

Giant liposome is an important lipid structure widely used in biological and medical fields. In its main preparation method, electroformation, many influencing factors must be optimized for good effect. How to collect the desired giant liposomes is another major issue. In this work, a microchip with a reactor chamber array was used to study the influences of multiple parameters, and a suitable condition could be achieved rapidly and efficiently. A tailor-made collection chamber was also integrated on the chip. Based on the multifactor and multilevel orthogonal experiment, optimal conditions of the lipid solution, buffer solution, and electric signal were achieved with high efficiency. More than one thousand giant liposomes could be formed in each microscale reactor chamber, and most of them were unilamellar. The on-chip collection ratio of giant liposome carriers could also approximate to 40%.
- Giant liposome,
- Microchip,
- ITO,
- Electroformation,
- Collection
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