Citation: Cao Dandan, Yin Yongguang, He Bin. Speciation of organomercury compounds by capillary electrophoresis with pre-column derivatization and on-line stacking[J]. Chinese Chemical Letters, ;2019, 30(3): 650-652. doi: 10.1016/j.cclet.2018.10.015 shu

Speciation of organomercury compounds by capillary electrophoresis with pre-column derivatization and on-line stacking

Cao Dandan ^a ,
Yin Yongguang ^a,b ,
He Bin ^a,b,*,

a.
State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

bhe@rcees.ac.cn

Received Date: 26 September 2018
Accepted Date: 16 October 2018
Available Online: 19 March 2018

Figures(4)

Simultaneous separation and detection of three organomercury species, namely methylmercury (MeHg), ethylmercury (EtHg), and phenylmercury (PhHg), was performed by using capillary electrophoresis (CE) with UV detection. Pre-column derivatization with thiosalicylic acid and on-line salt-induced stacking significantly improved the detection performance. Buffer pH, ion strength, and additive were optimized for CE separation, concentration of NaCl in sample solution and injection time were optimized for on-line stacking. The limits of detection were 76.9, 83.0 and 76.4 mg/L for PhHg, EtHg and MeHg, respectively. The developed method was validated by certified reference material and liquid chromatography-atomic fluorescence spectroscopy, which suggests this method could be useful in the speciation of organomercury compounds in biological samples.
- Capillary electrophoresis,
- Mercury speciation,
- Derivatization,
- On-line stacking
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沈阳化工大学材料科学与工程学院沈阳 110142