Citation: Liu Chenguang, Zheng Wenchen, Xie Ruoxiao, Liu Yupeng, Liang Zhe, Luo Guoan, Ding Mingyu, Liang Qionglin. Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers[J]. Chinese Chemical Letters, ;2019, 30(2): 457-460. doi: 10.1016/j.cclet.2018.09.010 shu

Microfluidic fabrication of water-in-water droplets encapsulated in hydrogel microfibers

a.
Key Laboratory of Chemical Biology (Ministry of Education), Beijing Key Laboratory of Microanalytical Methods & Instrumentation, Department of Chemistry, Tsinghua University, Beijing 100084, China
b.
State Key Laboratory of Quality Research in Chinese Medicine, Macau Institute for Applied Research in Medicine and Health, Macau University of Science and Technology, Macau, China

liangql@tsinghua.edu.cn

Received Date: 4 August 2018
Revised Date: 11 September 2018
Accepted Date: 12 September 2018
Available Online: 14 February 2018

Figures(5)

Aqueous microdroplets are of significant interest in biological fields, while the employment of water-inoil (w/o) emulsion and lack of spatial control precluded its widespread application. Herein a novel microfluidic approach is developed to generate water-in-water (w/w) microdroplets embedded in hydrogel microfibers. Aqueous two phase system (ATPS) is applied to generate w/w droplets, and alginate is introduced to continuous phase to form microfibers, which offers spatial restriction and manipulation possibility to droplets. The size and pattern of aqueous droplets can be precisely controlled, and immobilization within hydrogel fiber facilitates easy manipulation and observation. The microdroplets surrounded by hydrophilic environment can act as a cell-cell interaction model, and their potential for biological and environmental applications are demonstrated by long-term culture of encapsulated cells and water remediation of Bacillus subtilis.
- Microfluidics,
- Water-in-water droplets,
- Droplet fibers,
- Intercellular communication,
- Water remediation
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1.
沈阳化工大学材料科学与工程学院沈阳 110142