Citation: Xia Junmei, Wang Wenjing, Hai Xin, E Shuang, Shu Yang, Wang Jianhua. Improvement of antibacterial activity of copper nanoclusters for selective inhibition on the growth of gram-positive bacteria[J]. Chinese Chemical Letters, ;2019, 30(2): 421-424. doi: 10.1016/j.cclet.2018.07.008 shu

Improvement of antibacterial activity of copper nanoclusters for selective inhibition on the growth of gram-positive bacteria

Xia Junmei ^a ,
Wang Wenjing ^b ,
Hai Xin ^a ,
E Shuang ^a ,
Shu Yang ^a,*, ,
Wang Jianhua ^a,*,

a.
Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China
b.
College of Chemistry and Chemical Engineering, Qingdao University, Qingdao 266071, China

shuyang@mail.neu.edu.cn

jianhuajrz@mail.neu.edu.cn

Received Date: 31 May 2018
Revised Date: 2 July 2018
Accepted Date: 4 July 2018
Available Online: 5 February 2018

Figures(4)

In general, copper nanoclusters (CuNCs) possess very low or even virtually no bactericidal effect. Herein, we report a novel CuNCs possessing significantly high antibacterial activity, that is tannic acid (TA) capped CuNCs (TA-CuNCs). TA-CuNCs exhibit strong absorption and excitation-dependent fluorescence within pH 2-12, resulting from the functional groups of TA-CuNCs due to two prototropic equilibria, phenol↔phenolate and carboxylic↔carboxylate. There exists synergistic effect of TA and copper nanoclusters which endows TA-CuNCs remarkable antibacterial capability as a microbicide, as characterized by the effective inhibition on the growth of gram-positive bacteria by damaging the cell membrane. By incubating 1×10⁷ CFU/mL of gram-positive bacteria Staphylococcus aureus and Bacillus subtilis with 30 μg/mL of TA-CuNCs for 10 min, the bacteria are completely inhibited, while under same conditions the viabilities of gram-negative bacteria Escherichia coli O157:H7 and Pseudomonas aeruginosa remain 85.0%, 72.0%, respectively. In addition, TA-CuNCs exhibit low cytotoxicity and favorable biocompatibility demonstrated by standard methyl thiazolyl tetrazolium (MTT) assay with HepG2 and 293 T cells, giving rise to cell viability of 94.2% for HepG2 and 96.7% for 293 T by incubating 10⁶ cell/mL with 200 μg/mL of TA-CuNCs for 24 h. These results make TA-CuNCs a potential alternative as bactericide for infection treatment caused by gram-positive bacteria.
- Tannic acid capping,
- Copper nanoclusters,
- Antibacterial effect,
- Gram-positive bacteria
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