Citation: Lv Yiwei, Li Tiegang, Guo Chengan, Sun Chenglong, Tang Fei, Huang Luojiao, Luo Zhigang, Li Xin, Zhang Ruiping, Zang Qingce, He Jiuming, Abliz Zeper. A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer[J]. Chinese Chemical Letters, ;2019, 30(2): 461-464. doi: 10.1016/j.cclet.2018.06.006 shu

A high-performance bio-tissue imaging method using air flow-assisted desorption electrospray ionization coupled with a high-resolution mass spectrometer

Lv Yiwei ^a ,
Li Tiegang ^a ,
Guo Chengan ^b ,
Sun Chenglong ^a ,
Tang Fei ^b ,
Huang Luojiao ^a ,
Luo Zhigang ^a ,
Li Xin ^a ,
Zhang Ruiping ^a ,
Zang Qingce ^a ,
He Jiuming ^a,*, ,
Abliz Zeper ^a,c

a.
State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
b.
State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100086, China
c.
Center for Imaging and Systems Biology, Minzu University of China, Beijing 100081, China

hejiuming@imm.ac.cn

Received Date: 22 April 2018
Revised Date: 2 June 2018
Accepted Date: 4 June 2018
Available Online: 6 February 2018

Figures(4)

Mass spectrometry imaging (MSI) technology can simultaneously obtain the spatial distribution of thousands of chemical compounds and has unique advantages compared to other techniques that allow mapping the surface of bio-tissue. Here, we combined an air flow-assisted desorption electrospray ionization (AFADESI) MSI device with a high-resolution mass spectrometer to optimize the system parameters and achieve more accurate spatial distribution characteristics for compounds of interest while investigating bio-tissue sections. The platform set-up, required instrumentation, sample pretreatment, parameter optimization and bio-tissue characterization are described and discussed. Finally, the parameter conditions that can provide optimal ionic intensity and enhanced resolution were confirmed. The reasonable resolution and sensitivity improvements of AFADESI-MSI have been achieved through tandem a high-resolution mass spectrometer system, therefore, it would be a promising technique for the bio-tissue imaging analysis.
- Mass spectrometry imaging,
- Air flow-assisted desorption electrospray,
- ionization,
- High-resolution mass spectrometry,
- Endogenous metabolites,
- Bio-tissue imaging
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