Citation: Qin Huihuan, Li Lingling, Li Kun, Yu Xiaoqi. Novel strategy of constructing fluorescent probe for MAO-B via cascade reaction and its application in imaging MAO-B in human astrocyte[J]. Chinese Chemical Letters, ;2019, 30(1): 71-74. doi: 10.1016/j.cclet.2018.05.018 shu

Novel strategy of constructing fluorescent probe for MAO-B via cascade reaction and its application in imaging MAO-B in human astrocyte

Key Laboratory of Green Chemistry and Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China

Corresponding author: Li Kun, kli@scu.edu.cn Yu Xiaoqi, xqyu@scu.edu.cn
Received Date: 20 March 2018
Revised Date: 8 May 2018
Accepted Date: 8 May 2018
Available Online: 16 January 2018

Figures(7)

To detect monoamine oxidase B (MAO-B), the level of which is one of the most important indicators of neurodegenerative diseases such as Parkinson's disease, a new type of cascade reaction based on the formation of coumarin was applied in this research. After the reaction with MAO-B, the protecting group of hydroxyl group in probes (DEAN-MA and DEAB-MA) was removed, and the fluorescence intensity significantly increased (λ_em=456 nm) as the structure of coumarin was formed subsequently. The probes showed excellent sensitivity and selectivity to MAO-B. The detection limit of DEAN-MA and DEAB-MA were 0.6 ng/mL and 0.2 ng/mL, respectively. We succeeded in detecting MAO-B in vitro and imaging it in human astrocyte (U 87).
- Monoamine oxidase-B,
- Fluorescent probe,
- Covalent-assembly,
- Coumarin,
- Cell imaging
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