Citation: Zhang Mengge, Wen Ming, Xiong Ying, Zhang Longhua, Tian Changlin. In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺[J]. Chinese Chemical Letters, ;2018, 29(10): 1509-1512. doi: 10.1016/j.cclet.2018.04.026 shu

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺

Zhang Mengge ^a ,
Wen Ming ^a ,
Xiong Ying ^a ,
Zhang Longhua ^a,, ,
Tian Changlin ^a,b,*,,

a.
National Laboratory of Physical Sciences at Microscale and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China
b.
High Magnetic Field Laboratory, Chinese Academy of Sciences, Hefei 230031, China

Corresponding author: Zhang Longhua, zlhustc@ustc.edu.cn Tian Changlin, cltian@ustc.edu.cn

Received Date: 7 April 2018
Revised Date: 19 April 2018
Accepted Date: 23 April 2018
Available Online: 24 October 2018

Figures(4)

Fluorescence microscopy, as a sensitive method to detect microenvironment of molecules, is widely used in protein conformation and dynamic studies in live cells. Fluorescence lifetime imaging microscopy (FLIM), which is independent of fluorophore concentrations, scattering and bleaching, is a suitable tool to analyze membrane proteins in a single cell. Ferroportin (FPN), a multi-ion exporter in vertebrates, was modulated by metal ions with unknown mechanism. Herein, we fused green fluorescence protein on Cterminal of FPN (FPN-eGFP) and applied fluorescence lifetime to monitor conformation changes of FPN in a live cell. The fluorescence lifetime distribution showed a shift to shorter lifetime upon Mn²⁺ treatment, suggesting a preference conformation of FPN in Mn²⁺ exposure. It is also observed that the lifetime (rather than intensity) measurement was not strongly influenced by laser power. The observed fluorescence lifetime changes of FPN-eGFP upon Mn²⁺ treatments indicated that extracellular metal ions can modulate FPN through conformation exchanges between several different states.
- Fluorescence lifetime imaging microscopy,
- In cell analysis of conformation change,
- Ferroprotin
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沈阳化工大学材料科学与工程学院沈阳 110142

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn2+

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通讯作者: 陈斌, bchen63@163.com

In cell measurement of fluorescence lifetime imaging microscopy revealed C-terminal conformation changes of Ferroportin upon addition of Mn²⁺