Citation: Zhao Liping, Qiao Juan, Zhang Hongyi, Xie Fangnan, Qi Li. Open tubular capillary electrochromatography with block co-polymer coating for separation of β-lactam antibiotics[J]. Chinese Chemical Letters, ;2019, 30(2): 349-352. doi: 10.1016/j.cclet.2018.03.007 shu

Open tubular capillary electrochromatography with block co-polymer coating for separation of β-lactam antibiotics

Zhao Liping ^a,b ,
Qiao Juan ^b,c ,
Zhang Hongyi ^a,**, ,
Xie Fangnan ^d ,
Qi Li ^b,c,*,

a.
College of Chemistry & Environmental Science, Key Laboratory of Analytical Science and Technology of Hebei Province, Hebei University, Baoding 071002, China
b.
Beijing National Laboratory of Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China
c.
University of Chinese Academy of Sciences, Beijing 100049, China
d.
National Center for Children's Health, Beijing Children's Hospital, Beijing 100045, China

hyzhang@hbu.edu.cn

qili@iccas.ac.cn

Received Date: 6 January 2018
Revised Date: 9 February 2018
Accepted Date: 6 March 2018
Available Online: 8 February 2018

Figures(3)

A new open-tubular capillary electrochromatography (OT-CEC) method for analysis of β-lactam antibiotics has been developed with unique block co-polymer coating. To obtain the highly ordered block polymer chains, reversible addition fragmentation chain transfer radical polymerization method was used to synthesize poly(maleic anhydride-styrene-N-isopropylacrylamide). The prepared block copolymer coating was characterized with NMR, fourier transform infrared spectroscopy and scanning electron microscope. Several key separation factors of OT-CEC, which including polymer amount, stability of the coating, temperature, species of organic additives, buffer pH and concentration, were investigated in detail. Our results indicated that the separation efficiency was improved greatly with the coating capillary and the three test analytes could be baseline separated. Then, the separation mechanism was briefly explored. Moreover, the proposed OT-CEC method displayed promising quantitative analysis property of the three test analytes with good linearity (R² > 0.99), repeatability (relative standard deviations < 0.9%) and high recovery (95.4%-106.2%). Further, the assay was applied in monitoring the three test β-lactam antibiotics (cephradine, cephalexin and amoxicillin) in serum samples, providing a useful platform for construction of novel polymer coatings in OT-CEC system and for analysis of drugs in real bio-samples.
- Open-tubular capillary,
- Electrochromatography,
- β-Lactam antibiotics,
- Block co-polymer coating,
- Serum drugs separation
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