Citation: Dai Lingzi, Guo Nian, Liu Yaqin, Shen Shanshan, Ge Qiufu, Pan Yuanjiang. Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS[J]. Chinese Chemical Letters, ;2019, 30(1): 103-106. doi: 10.1016/j.cclet.2017.12.023 shu

Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

a.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China
b.
Hangzhou Pharmaceutical Group Co., Ltd., Hangzhou 311100, China

Corresponding author: Pan Yuanjiang, panyuanjiang@zju.edu.cn
Received Date: 17 November 2017
Revised Date: 25 December 2017
Accepted Date: 27 December 2017
Available Online: 30 January 2017

Figures(4)

The binding between NL-101, a novel nitrogen mustard anti-cancer drug, with amino acids and peptides has been investigated by high performance liquid chromatography electrospray tandem mass spectrometry (HPLC/ESI-MS/MS). This study offers supporting data of the interaction among drug and amino acids and peptides, which could potentially explain the cytotoxic and mutagenic effects of the drug. Collision-induced dissociation (CID) experiment demonstrated that under the same collision energy, the amino group combined with NL-101 adducts are sensitive and often produce more fragment ions; the carboxyl group combined with NL-101 adducts are hard to break and display fewer fragment ions. In addition, when other group (like sulfhydryl group) of amino acids binds to NL-101, CID spectra show different fragmentation pattern. These differences could display structural information about the drug adducts and be utilized as location of the authentic binding sites.
- High-performance liquid chromatography/,
- tandem mass spectrometry,
- NL-101,
- Amino acids,
- Peptides,
- Binding sites
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沈阳化工大学材料科学与工程学院沈阳 110142