Citation: Zhang Lin, Xu Tengfei, Pi Zifeng, Zheng Meizhu, Song Fengrui, Liu Zhiqiang. Effects of the ambient fine particulate matter (PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS[J]. Chinese Chemical Letters, ;2019, 30(1): 90-94. doi: 10.1016/j.cclet.2017.11.019 shu

Effects of the ambient fine particulate matter (PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS

Zhang Lin ^a,b ,
Xu Tengfei ^a ,
Pi Zifeng ^a,, ,
Zheng Meizhu ^c ,
Song Fengrui ^a ,
Liu Zhiqiang ^a,,

a.
Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun Center of Mass Spectrometry, Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun 130022, China
b.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China
c.
The Central Laboratory, Changchun Normal University, Changchun 130032, China

Corresponding author: Pi Zifeng, mslab21@ciac.ac.cn Liu Zhiqiang, liuzq@ciac.ac.cn
Received Date: 26 September 2017
Revised Date: 10 November 2017
Accepted Date: 14 November 2017
Available Online: 16 January 2017

Figures(3)

In order to study the damage of ambient fine particulate matter (PM2.5) to organism in the environment, the method of urinary metabolomics was used to detect the potential endogenous metabolites and their related metabolic pathways to clarify the toxicity mechanisms. Particles of PM2.5 collected from the fibrous membrane were extracted ultrasonically and dissolved in physiological saline to prepare PM2.5 suspension. The solution of saline and fine particular matter was injected into the trachea of rats respectively. The rats were injected two times a week for four weeks. Samples of 24-h urine were collected at the 14th day after the end of exposure, and the ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for the metabolomics detection. Principal component analysis (PCA) was used to investigate the global metabolomic alterations and a clear separation in the scatter diagram were observed. 17 potential endogenous metablites were identified from urinary samples in rats by UPLC-Q-TOF-MS. The primary metabolism pathways involved pentose and glucuronate interconversions, starch and sucrose metabolism, tryptophan metabolism, tyrosine metabolism, phenylalanine metabolism, purine metabolism, acetaminophen metabolism pathway, retinol metabolism and valproic acid metabolism pathway. The results are helpful to understand the toxicological mechanisms of PM2.5 and screened out potential biomarkers of rats which exposure to PM2.5.
- Ambient fine particulate matter,
- UPLC-Q-TOF-MS,
- Metabolomics,
- Urine
1. [1]
  C.A. Pope, R.T. Burnett, M.J. Thun, et al., J. Am. Med. Assoc. 287(2002) 1132-1141. doi: 10.1001/jama.287.9.1132
2. [2]
  D. Hartono, B. Lioe, Y. Zhang, B. Li, et al., Sci. Rep. 7(2017) 644. doi: 10.1038/s41598-017-00449-5
3. [3]
  Y.L. Shi, L.H. Deng, J. Med. Bio. 28(2013) 127-134.
4. [4]
  Y.Q. Lu, Y.T. Yuan, J. Anhui. Agric. 41(2013) 13988-13989.
5. [5]
  S. Barnesa, H.P. Bentonj, K. Casazzac, et al., J. Mass Spectrom. 51(2016) 461-475. doi: 10.1002/jms.3782
6. [6]
  N. Psychogios, D.D. Hau, J. Peng, et al., PLoS. One 6(2011) e16957. doi: 10.1371/journal.pone.0016957
7. [7]
  O. Beckonert, H.C. Keun, T.M.D. Ebbels, et al., Nat. Protoc. 2(2007) 2692. doi: 10.1038/nprot.2007.376
8. [8]
  Z.H. Su, S.Q. Li, G.A. Zou, C.Y. Yu, et al., J. Pharm. Bio. Anal. 55(2011) 533-539. doi: 10.1016/j.jpba.2011.02.013
9. [9]
  D.Pawlak, A.Tankiewicz, T.Matys, etal., J.Physiol.Pharmacol.54(2003)175-189.
10. [10]
  D. Pawlak, A. Tankiewicz, W. Buczko, J. Physiol. Pharmacol. 52(2001) 755-766.
11. [11]
  D.H. Wang, S.M. Wang, L. Wei, et al., Adv. Physiol. Sci. 47(2016) 43-46.
12. [12]
  L.E. Goldstein, M.C. Leopold, X. Huang, et al., Biochemistry 39(2000) 7266-7275. doi: 10.1021/bi992997s
13. [13]
  S. Okuda, N. Nishiyama, H. Saito, et al., J. Neurochem. 70(1998) 299-307.
14. [14]
  E.P. Wang, Y. Sun, H. Yue, et al., Chin. J. Anal. Chem. 44(2016) 1410-1418.
15. [15]
  N.G. Buckman, J.O. Hill, R.J. Magee, et al., J. Chromatogr. A 284(1984) 441-446. doi: 10.1016/S0021-9673(01)87845-6
16. [16]
  G. Glorieux, R. Vanholder, Contrib. Nephrol. 168(2011) 117-128.
17. [17]
  T. Miyagaki, I. Aoyama, M. Ise, et al., Nephrol. Dial. Transpl. 15(2000) 1773-1781. doi: 10.1093/ndt/15.11.1773
18. [18]
  E.J. Want, I.D. Wilson, H. Gika, et al., Nat. Protoc. 5(2010) 1005-1018. doi: 10.1038/nprot.2010.50
19. [19]
  N.S. Jain, U.H.N. Dürr, A. Ramamoorthy, Chin. Chem. Lett. 26(2015) 407-415. doi: 10.1016/j.cclet.2015.03.001
1. [1]
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2. [2]
  Xiaoxiao Wang , Bolun Wang , Fenfen Ji , Jie Yan , Jiacheng Fang , Doudou Zhang , Ji Xu , Jing Ji , Xinran Hao , Hemi Luan , Yanjun Hong , Shulan Qiu , Min Li , Zhu Yang , Wenlan Liu , Xiaodong Cai , Zongwei Cai . Discovery of plasma biomarkers for Parkinson’s disease diagnoses based on metabolomics and lipidomics. Chinese Chemical Letters, 2024, 35(11): 109653-. doi: 10.1016/j.cclet.2024.109653
3. [3]
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4. [4]
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5. [5]
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9. [9]
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10. [10]
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11. [11]
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12. [12]
  Jun-Jie Fang , Zheng Liu , Yun-Peng Xie , Xing Lu . Superatomic Ag₅₈ nanoclusters incorporating a [MS₄@Ag₁₂]²⁺ (M = Mo or W) kernel show aggregation-induced emission. Chinese Chemical Letters, 2024, 35(10): 109345-. doi: 10.1016/j.cclet.2023.109345
13. [13]
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14. [14]
  Yong-Fang Shi , Sheng-Hua Zhou , Zuju Ma , Xin-Tao Wu , Hua Lin , Qi-Long Zhu . From [Ba3S][GeS4] to [Ba3CO3][MS4] (M = Ge, Sn): Enhancing optical anisotropy in IR birefringent crystals via functional group implantation. Chinese Journal of Structural Chemistry, 2025, 44(1): 100455-100455. doi: 10.1016/j.cjsc.2024.100455

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