Citation: Kang Yao, Fan Jiangli, Jin Qiang, Shi Chenhui, Du Jianjun, Peng Xiaojun. A α-KA fluorescent probe for discrimination of blood cancer serum[J]. Chinese Chemical Letters, ;2017, 28(10): 1991-1993. doi: 10.1016/j.cclet.2017.08.054 shu

A α-KA fluorescent probe for discrimination of blood cancer serum

a.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China
b.
Department of Hepatobiliary and Pancreatic Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China

Corresponding author: Fan Jiangli, fanjl@dlut.edu.cn
Received Date: 15 June 2017
Revised Date: 22 August 2017
Accepted Date: 30 August 2017
Available Online: 1 October 2017

Figures(5)

α-Ketoglutaric acid (α-KA) is an important metabolic intermediate in tricarboxylic acid circle in our body. The mutations of isocitrate dehydrogenase-1 (IDH1) and isocitrate dehydrogenase-2 (IDH2), however, will lead to the transformation of α-KA into 2-hydroxyglutarate (2-HG), which is confirmed to closely related to actue myeloid leukemia (AML). Therefore it is of great significance to detect α-KA level changes in serum. In this paper, a fluorescent "off-on" probe CH for α-KA was designed based on naphthalimide fluorophore by introducing a hydrazine group for α-KA recognition and a long alkyl amino chain to enhance PET efficiency and water solubility. Cetyltrimethyl ammonium bromide (CTAB) was added toform self-assembly micelles for accelerating the recognition process. CH shows a 28-fold fluorescence enhancement ((I-I₀)/I₀ at 550 nm) over other biological species by optimizing the chemical recognition process of CH with α-KA. Significantly, CH was successfully applied for thefluorescence discrimination of all kinds of blood cancer serum samples. This work would provide a potential method that is quick and convenient for sensing α-KA and may promote fluorescence detection in clinical diagnosis.
- Biomarker,
- Fluorescent probe,
- α-Ketoglutaricacid,
- Actue myeloid leukemia (AML),
- Serum detection
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1.
沈阳化工大学材料科学与工程学院沈阳 110142