Citation: Wang Zhangjie, Chi Lianli. Recent advances in mass spectrometry analysis of low molecular weight heparins[J]. Chinese Chemical Letters, ;2018, 29(1): 11-18. doi: 10.1016/j.cclet.2017.08.050 shu

Recent advances in mass spectrometry analysis of low molecular weight heparins

Wang Zhangjie ,
Chi Lianli ^,

National Glycoengineering Research Center, Shandong University, Ji'nan 250100, China

Corresponding author: Chi Lianli, lianlichi@sdu.edu.cn
Received Date: 13 June 2017
Revised Date: 25 July 2017
Accepted Date: 24 August 2017
Available Online: 31 January 2017

Figures(6)

Carbohydrates are important biomolecules and promising novel drug candidates, but their structural complexity and heterogeneity hinder their study and application. Low molecular weight heparins (LMWHs) are widely used as anticoagulants in the clinic and are considered the most successful carbohydrate-based drugs. They consist of both natural structures inherited from parent heparin and modified structures derived from depolymerization reactions. Sophisticated analytical methods are in great demand to elucidate the fine structure of LMWHs. This review summarizes recent progress in mass spectrometry (MS) that facilitates the in-depth structural characterization of LMWHs. The strategies can be sorted into top-down approaches and bottom-up approaches. In top-down approaches, the intact oligosaccharides are analyzed directly by hyphenated MS techniques to reveal their distribution and composition. Bottom-up approaches provide complementary structural information by analyzing partially enzymatically digested fragments or exhaustively degraded disaccharide building blocks of LMWHs. Advances in the MS/MS sequencing of short oligosaccharides and bioinformatics tools are also reviewed. Multidimensional analysis by MS offers a rigorous inspection of LMWHs, which is indispensable for ensuring drug quality and expanding their applications.
- LMWHs,
- MS,
- Structural characterization,
- Chain mapping,
- Sequence
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