Citation: Song Fengling, Liang Ri, Deng Jundie, Liu Zhiwei, Peng Xiaojun. Fine-tailoring the linker of near-infrared fluorescence probes for nitroreductase imaging in hypoxic tumor cells[J]. Chinese Chemical Letters, ;2017, 28(10): 1997-2000. doi: 10.1016/j.cclet.2017.08.023 shu

Fine-tailoring the linker of near-infrared fluorescence probes for nitroreductase imaging in hypoxic tumor cells

State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China

Corresponding author: Song Fengling, songfl@dlut.edu.cn
Received Date: 19 June 2017
Revised Date: 21 July 2017
Accepted Date: 14 August 2017
Available Online: 24 October 2017

Figures(8)

Imaging hypoxia using fluorescence probes for nitroreductase (NTR) have attracted much attention in last decade. At least three different linkers have been commonly used to connect the recognition unit and reporting unit in reported probes for NTR. Meanwhile, the linker is known to be a key factor for achieving best sensing performance. In this work, three near-infrared fluorescence probes CyNP-1, CyNP-2 and CyNP-3 were designed and synthesized from an aminocyanine dye CyNP. The three probes have the same recognition unit and same fluorescence reporting unit, but different linkers. CyNP-1 was found to have the best sensing performance for NTR with 40-fold of fluorescence enhancement. It is well investigated how the difference of the linkers brings out the different sensing performance by HPLC, MS and docking calculations. In the end, CyNP-1 was found to have good selectivity for NTR and used to imaging hypoxia in Hela cells.
- Hypoxia,
- Fluorescence probes,
- Nitroreductase,
- Near-infrared,
- Aminocyanine dyes
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沈阳化工大学材料科学与工程学院沈阳 110142