Citation: Cheng Dan, Pan Yue, Yin Bin-Cheng, Yuan Lin, Zhang Xiao-Bing. A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress[J]. Chinese Chemical Letters, ;2017, 28(10): 1987-1990. doi: 10.1016/j.cclet.2017.08.021 shu

A new fluorescent probe with ultralow background fluorescence for imaging of endogenous cellular selenol under oxidative stress

a.
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
b.
Lab of Biosystem and Microanalysis, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China

Corresponding author: Yuan Lin, lyuan@hnu.edu.cn; yuanlin_1982@126.com
Received Date: 12 June 2017
Revised Date: 20 July 2017
Accepted Date: 14 August 2017
Available Online: 19 October 2017

Figures(4)

A new fluorescent probe (Rhod-Sec) for selenol detection with ultralow background fluorescence have been developed in this paper, which showed a 380-fold off-on fluorescence response, and the nontoxic Rhod-Sec is well suitable for detecting and imaging both exogenous and endogenous selenol in living cells. It also can be applied to visualize the fluctuation of selenol in HepG2 cells through LPS-induced cells oxidation resistance.
- Fluorescent probe,
- Selenol,
- Fluorescent imaging,
- Oxidative stress
1. [1]
  L. Schomburg, U. Schweizer, J. Kçhrle, Cell. Mol. Life Sci. 61(2004) 1988-1995.
2. [2]
  C.M. Weekley, H.H. Harris, Chem. Soc. Rev. 42(2013) 8870-8894. doi: 10.1039/c3cs60272a
3. [3]
  L. Johansson, G. Gafvelin, E.S. Arner, Biochim. Biophys. Acta 1726(2005) 1-13. doi: 10.1016/j.bbagen.2005.05.010
4. [4]
  X. Du, C. Wang, Q. Liu, Curr. Top. Med. Chem. 16(2016) 835-848.
5. [5]
  M. Kucharzewski, J. Braziewicz, U. Majewska, S. Gozdz, Biol. Trace Elem. Res. 88(2002) 25-30. doi: 10.1385/BTER:88:1
6. [6]
  J.R. Encinar, D. Schaumloffel, Y. Ogra, R. Lobinski, Anal. Chem. 76(2004) 6635-6642. doi: 10.1021/ac049280h
7. [7]
  G. Ballihaut, L.E. Kilpatrick, W.C. Davis, Anal. Chem. 83(2011) 8667-8674. doi: 10.1021/ac2021147
8. [8]
  S.J. Fairweather-Tait, Y. Bao, M.R. Broadley, et al., Antioxid. Redox Signal. 14(2011) 1337-1383. doi: 10.1089/ars.2010.3275
9. [9]
  S. Tanguy, S. Grauzam, J. de Leiris, F. Boucher, Mol. Nutr. Food Res. 56(2012) 1106-1121. doi: 10.1002/mnfr.201100766
10. [10]
  D.L. Hatfield, P.A. Tsuji, B.A. Carlson, V.N. Gladyshev, Trends Biochem. Sci. 39(2014) 112-120. doi: 10.1016/j.tibs.2013.12.007
11. [11]
  W. Xu, Z.B. Zeng, J.H. Jiang, Y.T. Chang, L. Yuan, Angew. Chem. Int. Ed. 55(2016) 13658-13699. doi: 10.1002/anie.201510721
12. [12]
  S.L. Shen, X.P. Chen, X.F. Zhang, J.Y. Miao, B.X. Zhao, J. Mater. Chem. B 3(2015) 919-925.
13. [13]
  X. Gao, X. Li, L. Li, J. Zhou, H. Ma, Chem. Commun. 51(2015) 9388-9390. doi: 10.1039/C5CC02788H
14. [14]
  T.F. Brewer, C.J. Chang, J. Am. Chem. Soc. 137(2015) 10886-10889. doi: 10.1021/jacs.5b05340
15. [15]
  D. Cheng, Y. Pan, L. Wang, et al., J. Am. Chem. Soc. 139(2017) 285-292. doi: 10.1021/jacs.6b10508
16. [16]
  X. Chen, Y. Zhou, X. Peng, J. Yoon, Chem. Soc. Rev. 39(2010) 2120-2135. doi: 10.1039/b925092a
17. [17]
  K. Xu, M. Qiang, W. Gao, et al., Chem. Sci. 4(2013) 1079-1086. doi: 10.1039/c2sc22076h
18. [18]
  D. Jung, S. Maiti, J.H. Lee, J.H. Lee, J.S. Kim, Chem. Commun. 50(2014) 3044-3047. doi: 10.1039/c3cc49790a
19. [19]
  J. Liu, Y.Q. Sun, H. Zhang, et al., Chem. Sci. 5(2014) 3183-3188. doi: 10.1039/c4sc00838c
20. [20]
  F. Kong, B. Hu, Y. Gao, et al., Chem. Commun. 51(2015) 3102-3105. doi: 10.1039/C4CC06359G
21. [21]
  H. Maeda, K. Katayama, H. Matsuno, T. Uno, Angew. Chem. Int. Ed. 45(2006) 1810-1813. doi: 10.1002/(ISSN)1521-3773
22. [22]
  Q. Sun, S.H. Yang, L. Wu, et al., Anal. Chem. 88(2016) 6084-6091. doi: 10.1021/acs.analchem.6b01545
23. [23]
  B. Zhang, C. Ge, J. Yao, et al., J. Am. Chem. Soc. 137(2015) 757-769. doi: 10.1021/ja5099676
24. [24]
  M.X. Li, W.Y. Feng, Q.S. Zhai, G.Q. Feng, Biosens. Bioelectron. 87(2017) 894-900. doi: 10.1016/j.bios.2016.09.056
25. [25]
  S. Areti, S.K. Verma, J. Bellare, C.P. Rao, Anal. Chem. 88(2016) 7259-7267. doi: 10.1021/acs.analchem.6b01518
26. [26]
  Z. Huang, S. Ding, D. Yu, F. Huang, G. Feng, Chem. Commun. 50(2014) 9185-9187. doi: 10.1039/C4CC03818E
27. [27]
  M. Kamiya, D. Asanuma, E. Kuranaga, et al., J. Am. Chem. Soc.133(2011) 12960-12963. doi: 10.1021/ja204781t
28. [28]
  M. Sakabe, D. Asanuma, M. Kamiya, et al., J. Am. Chem. Soc. 135(2013) 409-414. doi: 10.1021/ja309688m
29. [29]
  G.V. Kryukov, S. Castellano, S.V. Novoselov, et al., Science 300(2003) 1439-1443. doi: 10.1126/science.1083516
30. [30]
  C.M. Weekley, H.H. Harris, Chem. Soc. Rev. 42(2013) 8870-8894. doi: 10.1039/c3cs60272a
31. [31]
  E. Olm, A.P. Fernandes, C. Hebert, et al., Proc. Natl. Acad. Sci. U. S. A. 106(2009) 11400-11405. doi: 10.1073/pnas.0902204106
32. [32]
  L. Bjorkhem-Bergman, K. Jonsson, L.C. Eriksson, et al., Biochem. Pharmacol. 63(2002) 1875-1884. doi: 10.1016/S0006-2952(02)00981-4
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