Citation: Miao Luyang, Zhang Lianhua, Jiao Lei, Tan Xiaofeng, Wei Qin, Li He. pH Readout enhanced ELISA for point-of-care testing of cardiac troponin I[J]. Chinese Chemical Letters, ;2017, 28(9): 1878-1880. doi: 10.1016/j.cclet.2017.04.018 shu

pH Readout enhanced ELISA for point-of-care testing of cardiac troponin I

Miao Luyang ^a,1 ,
Zhang Lianhua ^b,1 ,
Jiao Lei ^a ,
Tan Xiaofeng ^a ,
Wei Qin ^a ,
Li He ^a,,

a.
School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China
b.
Department of Urology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, China

Corresponding author: Li He, lihecd@gmail.com; chem_lih@ujn.edu.cn

Received Date: 23 February 2017
Revised Date: 23 March 2017
Accepted Date: 1 April 2017
Available Online: 28 September 2017

Figures(3)

Enzyme-linked immunosorbent assay (ELISA) as a conventional method for protein quantification has its characteristic properties, however, it is challenging to implement excellent portability and sensitivity at the same time. In this study, we described a pH ELISA using synthetic melanin nanoparticles (SMNPs) for the co-immobilization of glucose oxidase (GOx) and second antibody (Ab₂) as signal labels, portable pH meter as signal readout device for point-of-care testing (POCT) of cardiac troponin I (cTnI). In accordance with the varying amount of cTnI, following sandwich type immunoassay, proportional SMNPs-GOx-Ab₂ were immobilized specifically resulting in corresponding decrease of pH values owing to GOx loaded on SMNPs can high-efficiency convert glucose into gluconic acid. This assay is easy-to-use, portable, sensitive and able to realize POCT, affording a linear range from 0.5 pg/mL to 10 ng/mL and low limit of detection (LOD) of 0.15 pg/mL towards cTnI, which was demonstrated the significant promising in the early diagnosis and screening of acute myocardial infarction.
- pH meter,
- Synthetic melanin nanoparticles,
- Polydopamine nanospheres,
- Biomarker,
- Immunosensor
1. [1]
  L.C. Chen, X.T. Zeng, P. Si, et al., Anal. Chem. 86(2014) 4188-4195. doi: 10.1021/ac403635f
2. [2]
  X.M. Fu, Z.J. Liu, S.X. Cai, et al., Chin. Chem. Lett. 86(2016) 920-926.
3. [3]
  T.T. Song, W. Wang, L.L. Meng, et al., Chin. Chem. Lett. 28(2017) 226-230. doi: 10.1016/j.cclet.2016.07.021
4. [4]
  Q.L. Li, D.L. Liu, L. Xu, et al., ACS Appl. Mater. Inter 7(2015) 22719-22726. doi: 10.1021/acsami.5b07895
5. [5]
  H.N. Li, Y.W. Mu, J.R. Yan, et al., Anal. Chem. 87(2015) 2007-2015. doi: 10.1021/ac504589d
6. [6]
  W. Li, Y. Li, Y. Wu, et al., J. Biomed. Nanotech. 11(2015) 2050-2056. doi: 10.1166/jbn.2015.2104
7. [7]
  Z. Li, J. He, Y. Zhang, et al., Chin. Chem. Lett. 27(2016) 1771-1775. doi: 10.1016/j.cclet.2016.06.052
8. [8]
  C. Li, Y.C. Yang, D. Wu, et al., Chem. Science 7(2016) 3011-3016. doi: 10.1039/C5SC04256A
9. [9]
  W.Y. Sun, W.Y. Liu, L.B. Qu, Chin Chem, Lett. 18(2016) 1107-1110.
10. [10]
  G.L. Fu, S.T. Sanjay, M.W. Dou, et al., Nanoscale 8(2016) 5422-5427. doi: 10.1039/C5NR09051B
11. [11]
  S.M. Wang, L. Ge, X.R. Song, et al., Biosens. Bioelectron. 31(2012) 212-218. doi: 10.1016/j.bios.2011.10.019
12. [12]
  P. Bai, Y. Luo, Y. Li, et al., Chin J. Anal, Chem. 41(2013) 20-24. doi: 10.1016/S1872-2040(13)60620-9
13. [13]
  S.T. Sanjay, M.W. Dou, J.J. Sun, et al., Sci. Rep. 6(2016) 30474. doi: 10.1038/srep30474
14. [14]
  S. Bagherbaigi, E.P. Corcoles, D.H.B. Wicaksono, Anal. Methods 18(2014) 7175-7180.
15. [15]
  D. Kwon, J. Joo, S. Lee, et al., Anal. Chem. 85(2013) 12134-12137. doi: 10.1021/ac403329w
16. [16]
  Y. Zhang, J.N. Yang, J.F. Nie, et al., Chem. Commun. 52(2015) 3474-3477.
17. [17]
  R.F. Ye, C.Z. Zhu, Y. Song, et al., Small 12(2016) 3094-3100. doi: 10.1002/smll.v12.23
18. [18]
  W.B. Qiang, W. Li, X.Q. Li, et al., Chem. Sci. 5(2014) 3018-3024. doi: 10.1039/C4SC00085D
19. [19]
  D.Q. Fan, C.T. Wu, K. Wang, et al., Chem. Commun. 52(2016) 406-409. doi: 10.1039/C5CC06754E
20. [20]
  D.F. Chang, Y.F. Gao, L.J. Wang, et al., J. Colloid & Interface Science 463(2016) 279-287.
21. [21]
  S.N. Sarangi, S. Nozaki, S.N. Sahu, J. Biomed, Nanotech. 11(2015) 988-996.
22. [22]
  X. Han, S.H. Li, Z.L. Peng, et al., Physiologia Plantarum. 49(2016) 141-144.
23. [23]
  A. Chiu, W.K. Chan, S.H. Cheng, et al., Qjm Monthly Journal of the Association of Physicians. 92(1999) 711-718. doi: 10.1093/qjmed/92.12.711
24. [24]
  S.S. Wong, Ann. Clin. Lab. Sci. 26(1996) 301-312.
25. [25]
  W.H. Hu, G.L. He, H.H. Zhang, et al., Anal. Chem. 86(2014) 4488-4493. doi: 10.1021/ac5003905
26. [26]
  Y.L. Liu, K.L. Ai, J.H. Liu, et al., Adv. Mater. 25(2013) 1353-1359. doi: 10.1002/adma.v25.9
27. [27]
  S.A. Centeno, J. Shamir, J. Mol. Structure 873(2008) 149-159. doi: 10.1016/j.molstruc.2007.03.026
1. [1]
  Yao-Hua Gu , Yu Chen , Qing Li , Neng-Bin Xie , Xue Xing , Jun Xiong , Min Hu , Tian-Zhou Li , Ke-Yu Yuan , Yu Liu , Tang Tang , Fan He , Bi-Feng Yuan . Metabolome profiling by widely-targeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of chronic kidney disease. Chinese Chemical Letters, 2024, 35(11): 109627-. doi: 10.1016/j.cclet.2024.109627
2. [2]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
3. [3]
  Xiaoxiao Wang , Bolun Wang , Fenfen Ji , Jie Yan , Jiacheng Fang , Doudou Zhang , Ji Xu , Jing Ji , Xinran Hao , Hemi Luan , Yanjun Hong , Shulan Qiu , Min Li , Zhu Yang , Wenlan Liu , Xiaodong Cai , Zongwei Cai . Discovery of plasma biomarkers for Parkinson’s disease diagnoses based on metabolomics and lipidomics. Chinese Chemical Letters, 2024, 35(11): 109653-. doi: 10.1016/j.cclet.2024.109653
4. [4]
  Jiaxu Wang , Jinxie Zhang , Xiuping Wang , Jingying Wang , Lina Chen , Jiahui Cao , Wei Cao , Siyu Liang , Ping Luan , Ke Zheng , Xiao-Kun Ouyang , Li Gao , Xiaowen Ou , Fan Zhang , Meitong Ou , Lin Mei . CaCO₃-coated hollow mesoporous silica nanoparticles for pH-responsive fungicides release. Chinese Chemical Letters, 2024, 35(12): 109697-. doi: 10.1016/j.cclet.2024.109697
5. [5]
  Yuwen Zhu , Xiang Deng , Yan Wu , Baode Shen , Lingyu Hang , Yuye Xue , Hailong Yuan . Formation mechanism of herpetrione self-assembled nanoparticles based on pH-driven method. Chinese Chemical Letters, 2025, 36(1): 109733-. doi: 10.1016/j.cclet.2024.109733
6. [6]
  Zhaorui Song , Qiulian Hao , Bing Li , Yuwei Yuan , Shanshan Zhang , Yongkuan Suo , Hai-Hao Han , Zhen Cheng . NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers. Chinese Chemical Letters, 2025, 36(1): 109834-. doi: 10.1016/j.cclet.2024.109834
7. [7]
  Mingqi Wang , Shixin Fa , Jiate Yu , Guoxian Zhang , Yi Yan , Qing Liu , Qiuyu Zhang . Light-controlled protein imprinted nanospheres with variable recognition specificity. Chinese Chemical Letters, 2025, 36(2): 110124-. doi: 10.1016/j.cclet.2024.110124
8. [8]
  Xiaoshuai Wu , Bailei Wang , Yichen Li , Xiaoxuan Guan , Mingjing Yin , Wenquan Lv , Yin Chen , Fei Lu , Tao Qin , Huyang Gao , Weiqian Jin , Yifu Huang , Cuiping Li , Ming Gao , Junyu Lu . NIR driven catalytic enhanced acute lung injury therapy by using polydopamine@Co nanozyme via scavenging ROS. Chinese Chemical Letters, 2025, 36(2): 110211-. doi: 10.1016/j.cclet.2024.110211
9. [9]
  Hao Zhang , Haonan Qu , Ehsan Bahojb Noruzi , Haibing Li , Feng Liang . A nanocomposite film with layer-by-layer self-assembled gold nanospheres driven by cucurbit[7]uril for the selective transport of L-tryptophan and lysozyme. Chinese Chemical Letters, 2025, 36(1): 109731-. doi: 10.1016/j.cclet.2024.109731
10. [10]
  Shehla Khalid , Muhammad Bilal , Nasir Rasool , Muhammad Imran . Photochemical reactions as synthetic tool for pharmaceutical industries. Chinese Chemical Letters, 2024, 35(9): 109498-. doi: 10.1016/j.cclet.2024.109498
11. [11]
  Botao QU , Qian WANG , Xiaogang NING , Yuxin ZHOU , Ruiping ZHANG . Deeply penetrating photoacoustic imaging in tumor tissues based on dual-targeted melanin nanoparticle. Chinese Journal of Inorganic Chemistry, 2024, 40(5): 1025-1032. doi: 10.11862/CJIC.20230416
12. [12]
  Jieqiong Qin , Zhi Yang , Jiaxin Ma , Liangzhu Zhang , Feifei Xing , Hongtao Zhang , Shuxia Tian , Shuanghao Zheng , Zhong-Shuai Wu . Interfacial assembly of 2D polydopamine/graphene heterostructures with well-defined mesopore and tunable thickness for high-energy planar micro-supercapacitors. Chinese Chemical Letters, 2024, 35(7): 108845-. doi: 10.1016/j.cclet.2023.108845
13. [13]
  Haitao Yin , Liang Meng , Li Li , Jiamu Xiao , Longrui Liang , Nannan Huang , Yansong Shi , Angang Zhao , Jingwen Hou . Polydopamine-modified biochar supported polylactic acid and zero-valent iron affects the functional microbial community structure for 1,1,1-trichloroethane removal in simulated groundwater. Chinese Chemical Letters, 2025, 36(1): 110313-. doi: 10.1016/j.cclet.2024.110313
14. [14]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
15. [15]
  Shuang Li , Jiayu Sun , Guocheng Liu , Shuo Zhang , Zhong Zhang , Xiuli Wang . A new Keggin-type polyoxometallate-based bifunctional catalyst for trace detection and pH-universal photodegradation of phenol. Chinese Chemical Letters, 2024, 35(8): 109148-. doi: 10.1016/j.cclet.2023.109148
16. [16]
  Rui Wang , He Qi , Haijiao Zheng , Qiong Jia . Light/pH dual-responsive magnetic metal-organic frameworks composites for phosphorylated peptide enrichment. Chinese Chemical Letters, 2024, 35(7): 109215-. doi: 10.1016/j.cclet.2023.109215
17. [17]
  Yan Liu , Yang Wang , Jiayi Zhu , Xuxian Su , Xudong Lin , Liang Xu , Xiwen Xing . Employing pH-responsive RNA triplex to control CRISPR/Cas9-mediated gene manipulation in mammalian cells. Chinese Chemical Letters, 2024, 35(9): 109427-. doi: 10.1016/j.cclet.2023.109427
18. [18]
  Tongyu Zheng , Teng Li , Xiaoyu Han , Yupei Chai , Kexin Zhao , Quan Liu , Xiaohui Ji . A DIY pH Detection Agent Using Persimmon Extract for Acid-Base Discoloration Popularization Experiment. University Chemistry, 2024, 39(5): 27-36. doi: 10.3866/PKU.DXHX202309107
19. [19]
  Chuyuan Lin , Hui Lin , Lingxing Zeng . Optimization strategy for rechargeable Zn metal batteries over wide-pH aqueous electrolytes. Chinese Journal of Structural Chemistry, 2025, 44(1): 100407-100407. doi: 10.1016/j.cjsc.2024.100407
20. [20]
  Yujie Li , Ya-Nan Wang , Yin-Gen Luo , Hongcai Yang , Jinrui Ren , Xiao Li . Advances in synthetic biology-based drug delivery systems for disease treatment. Chinese Chemical Letters, 2024, 35(11): 109576-. doi: 10.1016/j.cclet.2024.109576

Get Citation

PDF

XML

Metrics

PDF Downloads(1)
Abstract views(601)
HTML views(22)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142