Citation: Leng Shuang, Qiao Qing-Long, Gao Yue, Miao Lu, Deng Wu-Guo, Xu Zhao-Chao. SNAP-tag fluorogenic probes for wash free protein labeling[J]. Chinese Chemical Letters, ;2017, 28(10): 1911-1915. doi: 10.1016/j.cclet.2017.03.034 shu

SNAP-tag fluorogenic probes for wash free protein labeling

Leng Shuang ^a,b ,
Qiao Qing-Long ^b,c ,
Gao Yue ^a ,
Miao Lu ^b,, ,
Deng Wu-Guo ^a,, ,
Xu Zhao-Chao ^b,,

a.
Institute of Cancer Stem Cell, Cancer Center, Dalian Medical University, Dalian 116044, China
b.
Key Laboratory of Separation Science for Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
c.
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116012, China

Corresponding author: Miao Lu, miaolu@dicp.ac.cn Deng Wu-Guo, dengwg@hotmail.com Xu Zhao-Chao, zcxu@dicp.ac.cn
Received Date: 23 February 2017
Revised Date: 5 March 2017
Accepted Date: 23 March 2017
Available Online: 29 October 2017

Figures(4)

Protein labeling by using a protein tag and tag-specific fluorescent probes is increasingly becoming a useful technique for the real-time imaging of proteins in living cells. SNAP-tag as one of the most prominent fusion tags has been widely used and already commercially available. Recently, various fluorogenic probes for SNAP-tag based protein labeling were reported. Owing to turn-on fluorescence response, fluorogenic probes for SNAP-tag minimize the fluorescence background caused by unreacted or nonspecifically bound probes and allow for direct imaging in living cells without wash-out steps. Thus, real-time analysis of protein localization, dynamics and interactions has been made possible by SNAP-tag fluorogenic probes. In this review, we describe the design strategies of fluorogenic probes for SNAP-tag and their applications in cellular protein labeling.
- Fluorogenic probes,
- Wash free,
- SNAP-tag,
- Protein labeling
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