Citation: Wei Jin-Qi, Liu Yun, Zhang Xue-Hui, Liang Wei-Wei, Zhou Tuan-Feng, Zhang Hua, Deng Xu-Liang. Enhanced critical-sized bone defect repair efficiency by combining deproteinized antler cancellous bone and autologous BMSCs[J]. Chinese Chemical Letters, ;2017, 28(4): 845-850. doi: 10.1016/j.cclet.2017.01.008 shu

Enhanced critical-sized bone defect repair efficiency by combining deproteinized antler cancellous bone and autologous BMSCs

Wei Jin-Qi ^a,b,1 ,
Liu Yun ^a,c,1 ,
Zhang Xue-Hui ^d,e,f,, ,
Liang Wei-Wei ^a ,
Zhou Tuan-Feng ^b ,
Zhang Hua ^b ,
Deng Xu-Liang ^a,e,f,,

a.
Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China
b.
First Clinical Division, Peking University School and Hospital of Stomatology, Beijing 100034, China
c.
Department of Prosthodonitcs, Peking University School and Hospital of Stomatology, Beijing 10081, China
d.
Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing 100081, China
e.
National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China
f.
Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Corresponding author: Zhang Xue-Hui, zhangxuehui914@163.com Deng Xu-Liang, kqdengxuliang@bjmu.edu.cn

Received Date: 1 September 2016
Revised Date: 18 December 2016
Accepted Date: 21 December 2016
Available Online: 12 April 2017

Figures(5)

Previously we have demonstrated that calcinated antler cancellous bone(CACB)has great potential for bone defect repair, due to its highly similar composition and architecture to natural extracellular bone matrix.This study is aiming at seeking for an optimal strategy of combined application of CACB and bone marrow mesenchymal stem cells(BMSCs)in bone defect repair.In vitro study demonstrated that CACB promoted the adhesion, spreading and viability of BMSCs.Increased extracellular matrix production and expression of osteogenic markers in BMSCs were observed when seeded on CACB scaffolds.The cells ceased to proliferation in the dual effect of CACB and osteogenic induction at the early stage of incubation. Hence synergistic effect of CACB combined with autologous undifferentiated BMSCs in rabbit mandible critical-sized defect repair was further evaluated.Histological analysis results showed that loading the CACB with autologous BMSCs resulted in enhanced new bone formation and angiogenesis when compared with implanted CACB alone.These findings indicate that the combination of CACB and autologous BMSCs should become potential routes to improve bone repair efficiency
- Calcinated antler cancellous bone,
- Bone marrow mesenchymal stromal cells,
- Xenogenic bone graft,
- Osteogenic differentiation,
- Bone tissue engineering
1. [1]
  H. F. Nasr, M. E. Aichelmann-Reidy, R. A. Yukna, Bone and bone substitutes, Periodontology 19(1999)(2000)74-86.
2. [2]
  Kon E., Muraglia A., Corsi A.. Autologous bone marrow stromal cells loaded onto porous hydroxyapatite ceramic accelerate bone repair in critical-size defects of sheep long bones[J]. J.Biomed.Mater.Res., 2000,49:328-337. doi: 10.1002/(ISSN)1097-4636
3. [3]
  Bareille R., M.H.Lafage-Proust , Faucheux C.. Various evaluation techniques of newly formed bone in porous hydroxyapatite loaded with human bone marrow cells implanted in an extra-osseous site[J]. Biomaterials, 2000,21:1345-1352. doi: 10.1016/S0142-9612(00)00015-6
4. [4]
  Gutwald R., Haberstroh J., Kuschnierz J.. Mesenchymal stem cells and inorganic bovine bone mineral in sinus augmentation:comparison with augmentation by autologous bone in adult sheep[J]. Br.J.Oral.Maxillofac.Surg., 2010,48:285-290. doi: 10.1016/j.bjoms.2009.06.226
5. [5]
  Zhang X., Cai Q., Liu H.. Osteoconductive effectiveness of bone graft derived from antler cancellous bone:an experimental study in the rabbit mandible defect model[J]. Int.J.Oral.Maxillofac.Surg., 2012,41:1330-1337. doi: 10.1016/j.ijom.2012.05.014
6. [6]
  Zhang X.H., Xu M.M., Song L.. Effects of compatibility of deproteinized antler cancellous bone with various bioactive factors on their osteogenic potential[J]. Biomaterials, 2013,34:9103-9114. doi: 10.1016/j.biomaterials.2013.08.024
7. [7]
  Wei J.Q., Xu M.M., Zhang X.H.. Enhanced osteogenic behavior of ADSCs produced by deproteinized antler cancellous bone and evidence for involvement of ERK signaling pathway[J]. Tissue Eng.Part A, 2015,21:1810-1821. doi: 10.1089/ten.tea.2014.0395
8. [8]
  Mizuno H. Adipose-derived stem cells for tissue repair and regeneration:ten years of research and a literature review[J]. J.Nippon Med.Sch., 2009,76:56-66. doi: 10.1272/jnms.76.56
9. [9]
  Yamada Y., Nakamura S., Ito K.. Injectable tissue-engineered bone using autogenous bone marrow-derived stromal cells for maxillary sinus augmentation:clinical application report from a 2-6-year follow-up[J]. Tissue Eng.A, 2008,14:1699-1707. doi: 10.1089/ten.tea.2007.0189
10. [10]
  Dallari D., Savarino L., Stagni C.. Enhanced tibial osteotomy healing with use of bone grafts supplemented with platelet gel or platelet gel and bone marrow stromal cells[J]. J.Bone Jt.Surg.Am., 2007,89:2413-2420.
11. [11]
  Gamie Z., Tran G.T., Vyzas G.. Stem cells combined with bone graft substitutes in skeletal tissue engineering[J]. Expert Opin.Biol.Ther., 2012,12:713-729. doi: 10.1517/14712598.2012.679652
12. [12]
  Karageorgiou V., Kaplan D. Porosity of 3D biomaterial scaffolds and osteogenesis[J]. Biomaterials, 2005,26:5474-5491. doi: 10.1016/j.biomaterials.2005.02.002
13. [13]
  Kasten P., Beyen I., Niemeyer P.. Porosity and pore size of b-tricalcium phosphate scaffold can influence protein production and osteogenic differentiation of human mesenchymal stem cells:an in vitro and in vivo study[J]. Acta Biomater., 2008,4:1904-1915. doi: 10.1016/j.actbio.2008.05.017
14. [14]
  Müller P., Bulnheim U., Bulnheim A.. Calcium phosphate surfaces promote osteogenic differentiation of mesenchymal stem cells[J]. J.Cell.Mol. Med., 2008,12:281-291.
15. [15]
  Cui L., Liu B., Liu G.P.. Repair of cranial bone defects with adipose derived stem cells and coral scaffold in a canine model[J]. Biomaterials, 2007,28:5477-5486. doi: 10.1016/j.biomaterials.2007.08.042
16. [16]
  Meng S., Zhang X.H., Xu M.M.. Effects of deer age on the physicochemical properties of deproteinized antler cancellous bone:an approach to optimize osteoconductivity of bone graft[J]. Biomed.Mater., 2015,10035006. doi: 10.1088/1748-6041/10/3/035006
17. [17]
  Sun H.L., Wu C.T., Dai K.R., Chang J., Tang T.T. Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics[J]. Biomaterials, 2006,27:5651-5657. doi: 10.1016/j.biomaterials.2006.07.027
18. [18]
  Amaral M., Costa M.A., Lopes M.A.. Si₃N₄-bioglass composites stimulate the proliferation of MG63 osteoblast-like cells and support the osteogenic differentiation of human bone marrow cells[J]. Biomaterials, 2002,23:4897-4906. doi: 10.1016/S0142-9612(02)00249-1
19. [19]
  Nair M.B., Bernhardt A., Lode A.. A bioactive triphasic ceramic-coated hydroxyapatite promotes proliferation and osteogenic differentiation of human bone marrow stromal cells[J]. J.Biomed.Mater.Res.A, 2009,90:533-542.
20. [20]
  Honda M., Kikushima K., Kawanobe Y.. Enhanced early osteogenic differentiation by silicon-substituted hydroxyapatite ceramics fabricated via ultrasonic spray pyrolysis route[J]. J.Mater.Sci.Mater.Med., 2012,23:2923-2932. doi: 10.1007/s10856-012-4744-x
21. [21]
  Luo X.M., Barbieri D., Davison N.. Zinc in calcium phosphate mediates bone induction:in vitro and in vivo model[J]. Acta Biomater., 2014,10:477-485. doi: 10.1016/j.actbio.2013.10.011
22. [22]
  Oh S.A., Kim S.H., Won J.E.. Effects on growth and osteogenic differentiation of mesenchymal stem cells by the zinc-added sol-gel bioactive glass granules[J]. J.Tissue Eng., 2011,2010475260.
23. [23]
  Dawson J.I., Oreffo R.O.C. Bridging the regeneration gap:stem cells, biomaterials and clinical translation in bone tissue engineering[J]. Arch. Biochem.Biophys., 2008,473:124-131. doi: 10.1016/j.abb.2008.03.024
24. [24]
  Lu Z.F., S.I.Roohani-Esfahani , Wang G.C., Zreiqat H. Bone biomimetic microenvironment induces osteogenic differentiation of adipose tissue-derived mesenchymal stem cells[J]. Nanomed.Nanotechnol.Biol.Med., 2012,8:507-515. doi: 10.1016/j.nano.2011.07.012
25. [25]
  Açil Y., Terheyden H., Dunsche A., Fleiner B., Jepsen S. Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral[J]. J.Biomed.Mater.Res., 2000,51:703-710. doi: 10.1002/(ISSN)1097-4636
26. [26]
  Kubler A., Neugebauer J., Oh J.H., Scheer M., Zöller J.E.. Growth and proliferation of human osteoblasts on different bone graft substitutes:an in vitro study[J]. Implant.Dent., 2004,13:171-179. doi: 10.1097/01.ID.0000127522.14067.11
27. [27]
  Siggers K., Frei H., Fernlund G., Rossi F. Effect of bone graft substitute on marrow stromal cell proliferation and differentiation[J]. J.Biomed.Mater.Res.A, 2010,94:877-885.
28. [28]
  Chandra V.S., Baskar G., Suganthi R.V.. Blood compatibility of iron-doped nanosize hydroxyapatite and its drug release[J]. ACS Appl.Mater.Interfaces, 2012,4:1200-1210. doi: 10.1021/am300140q
29. [29]
  Li H.Y., Xue K., Kong N., Liu K., Chang J. Silicate bioceramics enhanced vascularization and osteogenesis through stimulating interactions between endothelia cells and bone marrow stromal cells[J]. Biomaterials, 2014,35:3803-3818. doi: 10.1016/j.biomaterials.2014.01.039
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