Citation: Wei Jin-Qi, Liu Yun, Zhang Xue-Hui, Liang Wei-Wei, Zhou Tuan-Feng, Zhang Hua, Deng Xu-Liang. Enhanced critical-sized bone defect repair efficiency by combining deproteinized antler cancellous bone and autologous BMSCs[J]. Chinese Chemical Letters, ;2017, 28(4): 845-850. doi: 10.1016/j.cclet.2017.01.008 shu

Enhanced critical-sized bone defect repair efficiency by combining deproteinized antler cancellous bone and autologous BMSCs

Wei Jin-Qi ^a,b,1 ,
Liu Yun ^a,c,1 ,
Zhang Xue-Hui ^d,e,f,, ,
Liang Wei-Wei ^a ,
Zhou Tuan-Feng ^b ,
Zhang Hua ^b ,
Deng Xu-Liang ^a,e,f,,

a.
Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China
b.
First Clinical Division, Peking University School and Hospital of Stomatology, Beijing 100034, China
c.
Department of Prosthodonitcs, Peking University School and Hospital of Stomatology, Beijing 10081, China
d.
Department of Dental Materials & Dental Medical Devices Testing Center, Peking University School and Hospital of Stomatology, Beijing 100081, China
e.
National Engineering Laboratory for Digital and Material Technology of Stomatology, Peking University School and Hospital of Stomatology, Beijing 100081, China
f.
Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Corresponding author: Zhang Xue-Hui, zhangxuehui914@163.com Deng Xu-Liang, kqdengxuliang@bjmu.edu.cn

Received Date: 1 September 2016
Revised Date: 18 December 2016
Accepted Date: 21 December 2016
Available Online: 12 April 2017

Figures(5)

Previously we have demonstrated that calcinated antler cancellous bone(CACB)has great potential for bone defect repair, due to its highly similar composition and architecture to natural extracellular bone matrix.This study is aiming at seeking for an optimal strategy of combined application of CACB and bone marrow mesenchymal stem cells(BMSCs)in bone defect repair.In vitro study demonstrated that CACB promoted the adhesion, spreading and viability of BMSCs.Increased extracellular matrix production and expression of osteogenic markers in BMSCs were observed when seeded on CACB scaffolds.The cells ceased to proliferation in the dual effect of CACB and osteogenic induction at the early stage of incubation. Hence synergistic effect of CACB combined with autologous undifferentiated BMSCs in rabbit mandible critical-sized defect repair was further evaluated.Histological analysis results showed that loading the CACB with autologous BMSCs resulted in enhanced new bone formation and angiogenesis when compared with implanted CACB alone.These findings indicate that the combination of CACB and autologous BMSCs should become potential routes to improve bone repair efficiency
- Calcinated antler cancellous bone,
- Bone marrow mesenchymal stromal cells,
- Xenogenic bone graft,
- Osteogenic differentiation,
- Bone tissue engineering
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