Citation: Hou Guang-Yue, Men Li-Hui, Wang Lu, Zheng Zhong, Liu Zhi-Qiang, Song Feng-Rui, Liu Zhong-Ying. Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats[J]. Chinese Chemical Letters, ;2017, 28(6): 1214-1219. doi: 10.1016/j.cclet.2016.12.039 shu

Quantitative analysis of urinary endogenous markers for the treatment effect of Radix Scutellariae on type 2 diabetes rats

a.
National Center for Mass Spectrometry in Changchun & Jilin Province Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
b.
School of Pharmaceutical Sciences, Jilin University, Changchun 130012, China

Corresponding author: Song Feng-Rui, songfr@ciac.ac.cn

Received Date: 30 August 2016
Revised Date: 17 November 2016
Accepted Date: 1 December 2016
Available Online: 8 June 2017

Figures(5)

The effect of Radix Scutellariae treated on type 2 diabetic rats has been investigated by a liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) based urinary quantitative approach. In this research, multiple reactions monitoring mode of MS/MS in LC-MS/MS analysis was used to quantitatively analyze the concentrations of 7 endogenous compounds in urine of normal control group, type 2 diabetic model group and Radix Scutellariae-treated group, and multivariate statistical analysis was utilized for MS data processing. The above-mentioned three groups can be distinguished via pattern recognition. The obtained results indicated that Radix Scutellariae affect the urinary metabolic profiling of type 2 diabetic rats on the polyol pathway, protein glycation reaction and amino acids metabolism pathway. According to these results, Radix Scutellariae should have the pharmacological effect on preventing or delaying the onset and progression of diabetes and its complications.
- LC-MS/MS,
- Endogenous markers,
- Multivariate statistical analysis,
- Type 2 diabetic complications,
- Radix Scutellariae
1. [1]
  Porte Jr D., Schwartz M.W.. Diabetes complications-why is glucose potentially toxic[J]. Science, 1996:272-699.
2. [2]
  Stratton I.M., Adler A.I., Neil H.A.W.. Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35):prospective observational study[J]. BMJ, 2000,321:405-412. doi: 10.1136/bmj.321.7258.405
3. [3]
  Ramasamy R., Goldberg I.J.. Aldose reductase and cardiovascular diseases, creating human-like diabetic complications in an experimental model[J]. Circ. Res., 2010,106:1449-1458. doi: 10.1161/CIRCRESAHA.109.213447
4. [4]
  Brownlee M.. Biochemistry and molecular cell biology of diabetic complications[J]. Nature, 2001,414:813-820. doi: 10.1038/414813a
5. [5]
  Ton A.L.Y., Forbes J.M., Cooper M.E.. AGE, RAGE, and ROS in diabetic nephropathy[J]. Semin. Nephrol., 2007,27:130-143. doi: 10.1016/j.semnephrol.2007.01.006
6. [6]
  Chung S.S.M., Ho E.C.M., Lam K.S.L., Chung S.K.. Contribution of polyol pathway todiabetes-induced oxidative stress[J]. J. Am. Soc. Nephrol., 2003,14:S233-S236. doi: 10.1097/01.ASN.0000077408.15865.06
7. [7]
  Inoguchi T., Sonta T., Tsubouchi H.. Protein kinase C-dependent increase in reactive oxygen species (ROS) production in vascular tissues of diabetes:role of vascular NAD(P)H oxidase[J]. J. Am. Soc. Nephrol., 2003,14:S227-S232.
8. [8]
  Maccari R., Ottanà R.. Targeting aldose reductase for the treatment of diabetes complications and inflammatory diseases:new insights and future directions[J]. J. Med. Chem., 2015,58:2047-2067. doi: 10.1021/jm500907a
9. [9]
  Magalhães P.M., Appell H.J., Duarte J.A.. Involvement of advanced glycation end products in the pathogenesis of diabetic complications:the protective role of regular physical activity[J]. Eur. Rev. Aging Phys. Act., 2008,5:17-29. doi: 10.1007/s11556-008-0032-7
10. [10]
  Choudhuri S., Dutta D., Sen A.. Role of N-ε-carboxy methyl lysine, advanced glycation end products and reactive oxygen species for the development of nonproliferative and proliferative retinopathy in type 2 diabetes mellitus[J]. Mol. Vis., 2013,19:100-113.
11. [11]
  Wang C.Y., Zhu H.B., Pi Z.F.. Classification of type 2 diabetes rats based on urine amino acids metabolic profiling by liquid chromatography coupled with tandem mass spectrometry[J]. J. Chromatogr. B, 2013,935:26-31. doi: 10.1016/j.jchromb.2013.07.016
12. [12]
  Song K.H., Lee S.H., Kim B.Y., Park A.Y., Kim J.Y.. Extracts of Scutellaria baicalensis reduced body weight and blood triglyceride in db/db Mice[J]. Phytother. Res., 2013,27:244-250. doi: 10.1002/ptr.v27.2
13. [13]
  Suh K.S., Nam Y.H., Ahn Y.M.. Effect of scutellariae radix extract on the high glucose-induced apoptosis in cultured vascular endothelial cells[J]. Biol. Pharm. Bull., 2003,26:1629-1632. doi: 10.1248/bpb.26.1629
14. [14]
  Zhang D.Y., Wu J., Ye F.. Inhibition of cancer cell proliferation and prostaglandin E₂ synthesis by Scutellaria baicalensis[J]. Cancer Res., 2003,63:4037-4043.
15. [15]
  Li H.B., Jiang Y., Chen F.. Separation methods used for Scutellaria baicalensis active components[J]. J. Chromatogr. B, 2004,812:277-290. doi: 10.1016/S1570-0232(04)00545-8
16. [16]
  Zhu Z.Y., Zhao L.A., Liu X.F.. Comparative pharmacokinetics of baicalin and wogonoside by liquid chromatography-mass spectrometry after oral administration of Xiaochaihu Tang and Radix scutellariae extract to rats[J]. J. Chromatogr. B, 2010,878:2184-2190. doi: 10.1016/j.jchromb.2010.06.021
17. [17]
  Ahad A., Mujeeb M., Ahsan H., Siddiqui W.A.. Prophylactic effect of baicalein against renal dysfunction in type 2 diabetic rats[J]. Biochimie, 2014,106:101-110. doi: 10.1016/j.biochi.2014.08.006
18. [18]
  Park S.M., Hong S.M., Sung S.R., Lee J.E., Kwon D.Y.. Extracts of Rehmanniae radix, Ginseng radix and Scutellariae radix improve glucose-stimulated insulin secretionand β-cell proliferation through IRS2 induction[J]. Genes Nutr., 2008,2:347-351. doi: 10.1007/s12263-007-0065-y
19. [19]
  El-Bassossy H.M., Hassan N.A., Mahmoud M.F., Fahmy A.. Baicalein protects against hypertension associated with diabetes:effect on vascular reactivity and stiffness[J]. Phytomedicine, 2014,21:1742-1745. doi: 10.1016/j.phymed.2014.08.012
20. [20]
  Hou G.Y., Zhang R.X., Pi Z.F.. A new method for screeningaldose reductase inhibitors using ultrahigh performance liquid chromatography-tandem mass spectrometry[J]. Anal. Methods, 2014,6:7681-7688. doi: 10.1039/C4AY00857J
21. [21]
  Pérez-Enciso M., Tenenhaus M.. Prediction of clinical outcomewith microarray data:a partial least squares discriminant analysis (PLS-DA) approach[J]. Hum. Genet., 2003,112:581-592.
22. [22]
  Hsu F.F., Turk J.. Characterization of phosphatidylethanolamine as a lithiated adduct by triple quadrupole tandem mass spectrometry with electrospray ionization[J]. J. Mass Spectrom., 2000,35:596-606.
23. [23]
  Wilson D.K., Bohren K.M., Gabbay K.H., Quiocho F.A.. An unlikely sugar substrate site in the 1.65A structure of the human aldose reductase holoenzyme implicated in diabetic complications[J]. Science, 1992,257:81-84. doi: 10.1126/science.1621098
24. [24]
  Goh S.Y., Cooper M.E.. The role of advanced glycation end products in progression and complications of diabetes[J]. J. Clin. Endocrinol. Metab., 2008,93:1143-1152. doi: 10.1210/jc.2007-1817
25. [25]
  Ahmed N., Babaei-Jadidi R., Howell S.K., Beisswenger P.J., Thornalley P.J.. Degradation products of proteins damaged by glycation, oxidation and nitration in clinical type 1 diabetes[J]. Diabetologia, 2005,48:1590-1603. doi: 10.1007/s00125-005-1810-7
26. [26]
  Hou G.Y., Wang L., Liu S., Song F.R., Liu Z.Q.. Inhibitory effect of eleven herbal extracts on advanced glycation end-products formation and aldose reductase activity[J]. Chin. Chem. Lett., 2014,25:1039-1043. doi: 10.1016/j.cclet.2014.04.029
27. [27]
  Piatti P.M., Monti L.D., Valsecchi G.. Long-term oral L-arginine administration improves peripheral and hepatic insulin sensitivity in type 2 diabetic patients[J]. Diabetes Care, 2001,24:875-880. doi: 10.2337/diacare.24.5.875
28. [28]
  Malloy V.L., Krajcik R.A., Bailey S.J.. Methionine restriction decreases visceral fat mass and preserves insulin action in aging male Fischer 344 rats independent of energy restriction[J]. Aging Cell, 2006,5:305-314. doi: 10.1111/ace.2006.5.issue-4
29. [29]
  Mihalik S.J., Michaliszyn S.F., de las Heras J.. Metabolomic profiling of fatty acid and amino acid metabolism in youth with obesity and type 2 diabetes evidence for enhanced mitochondrial oxidation[J]. Diabetes Care, 2012,35:605-611. doi: 10.2337/DC11-1577
30. [30]
  Qu Y., Slocum R.H., Fu J.. Quantitative amino acid analysis using a Beckman system gold HPLC 126AA analyzer[J]. Clin. Chim. Acta, 2001,312:153-162. doi: 10.1016/S0009-8981(01)00615-5
31. [31]
  Petrie J.R., Ueda S., Webb D.J., Elliott H.L., Connel J.M.C.. Endothelial nitric oxide production and insulin sensitivity-Aphysiological link with implications for pathogenesis of cardiovascular disease[J]. Circulation, 1996,93:1331-1333. doi: 10.1161/01.CIR.93.7.1331
32. [32]
  Rajapakse N.W., Chong A.L., Zhang W.Z., Kaye D.M.. Insulin-mediated activation of the L-arginine nitric oxide pathway in man, and its impairment in diabetes[J]. PLoS One, 2013,8e61840. doi: 10.1371/journal.pone.0061840
33. [33]
  Tuitoek P.J., Ziari S., Tsin A.T., Rajotte R.V.. Streptozotocin-induced diabetes in rats is associated with impaired metabolic availability of vitamin A (retinol)[J]. Br. J. Nutr., 1996,75:615-622. doi: 10.1079/BJN19960164
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