Citation: Fu Yuan-Hui, Liu Ya-Ru, Zheng Yan-Peng, Jiang Nan, Yue-Ying-Jiao, Li Wei, Peng Xiang-Lei, He Jin-Sheng. An RNA polymerase I-driven human respiratory syncytial virus minigenome as a tool for quantifying virus titers and screening antiviral drug[J]. Chinese Chemical Letters, ;2017, 28(1): 131-135. doi: 10.1016/j.cclet.2016.06.018 shu

An RNA polymerase I-driven human respiratory syncytial virus minigenome as a tool for quantifying virus titers and screening antiviral drug

College of Life Sciences and Bioengineering, Beijing Jiaotong University, Beijing 100044, China

Corresponding author: He Jin-Sheng, jshhe@bjtu.edu.cn

Received Date: 18 April 2016
Revised Date: 15 May 2016
Accepted Date: 26 May 2016
Available Online: 22 January 2016

Figures(6)

Human respiratory syncytial virus (RSV) is an important pediatric pathogen of lower respiratory tract worldwide. No vaccines and antiviral drugs are available. Herein the use of an RNA polymerase I-driven RSV minigenome for analyzing RSV replication and screening anti-RSV drugs was investigated. The RNA polymerase I (Pol I) was used to transcribe RSV minigenome from the constructed plasmid, designated pHM-RSV-Gluc, of minigenome cDNA which comprised trailer region, gene start sequence (GS), reverse complementary copy of Gaussia luciferase (Gluc) gene, gene end sequence (GE), and leader region in the direction of 5'-3' end and was flanked by promoter and terminator of Pol I. The expression of Gluc was confirmed in pHM-RSV-Gluc transfected HEp-2 cells following RSV infection and had the characteristics of dose-dependent, which provided a rapid, sensitive, and quantitative method for quantifying virus titers and screening antiviral drugs.
- Human respiratory syncytial virus,
- Gaussia luciferase,
- Minigenome,
- Antiviral drug,
- Drug-screening
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