Citation: Li Wang, Xu Sheng-Wei, Xu Hui-Ren, Song Yi-Lin, Liu Jun-Tao, Luo Jin-Ping, Cai Xin-Xia. Spatio-temporally resolved measurement of quantal exocytosis from single cells using microelectrode array modified with poly L-lysine and poly dopamine[J]. Chinese Chemical Letters, ;2016, 27(5): 738-744. doi: 10.1016/j.cclet.2016.01.018 shu

Spatio-temporally resolved measurement of quantal exocytosis from single cells using microelectrode array modified with poly L-lysine and poly dopamine

Li Wang ^a ,
Xu Sheng-Wei ^a,b ,
Xu Hui-Ren ^a,b ,
Song Yi-Lin ^a ,
Liu Jun-Tao ^a ,
Luo Jin-Ping ^a ,
Cai Xin-Xia ^a,b,,

a.
State Key Laboratory of Transducer Technology, Institute of Electronics, Chinese Academy of Sciences, Beijing 100190, China
b.
University of Chinese Academy of Sciences, Beijing 100190, China

Corresponding author: Cai Xin-Xia, xxcai@mail.ie.ac.cn
Received Date: 30 September 2015
Revised Date: 26 October 2015
Accepted Date: 22 January 2015
Available Online: 22 May 2016

Figures(6)

The subsecond, temporal, vesicular exocytosis is ubiquitous, but difficult detecting in communication mechanisms of cells. A microelectrode array (MEA), fabricated by MEMS technology, was applied successfully for real-time monitoring of quantal exocytosis from single pheochromocytoma (PC12) cell. The developed MEA was evaluated by dopamine (DA) using electrochemical methods and the results revealed that the sensitivity of DA was improved to 12659.24 μA L mmol^-1 cm^-2. The modified MEA was used to detect in vitro vesicular exocytosis of DA from single PC12 cells stimulated by concentrated 100 μmol L^-1 K⁺ cell solution. A total of 592 spikes were measured and analyzed by three parameters and the statistical results revealed the population of each parameter was an approximate Gaussian distribution, and on average, 1.31×10⁶±9.25×10⁴ oxidizable molecules were released in each quantal exocytosis. In addition, results also indicate that a single PC12 cell probably releases the spikes with T ranging from 25.6 ms to 35.4 ms corresponding to I_max ranging from 45.6 pA to 65.2 pA. The devices, including a homemade computer interface and the MEA modified with polymer film, provides a new means for further research on the neural, intercellular, communication mechanism.
- Microelectrode array,
- Quantal exocytosis,
- Dopamine,
- Parameter
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