Citation: Fei Tang, Yi Chen, Jiu-Ming He, Zhi-Gang Luo, Zeper Abliz, Xiao-Hao Wang. Design and performance of air flow-assisted ionization imaging mass spectrometry system[J]. Chinese Chemical Letters, ;2014, 25(05): 687-692. doi: 10.1016/j.cclet.2014.01.046 shu

Design and performance of air flow-assisted ionization imaging mass spectrometry system

1.
a State Key Laboratory of Precision Measurement Technology and Instruments, Department of Precision Instrument, Tsinghua University, Beijing 100086, China;
2.
b State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China

Corresponding author: Xiao-Hao Wang,
Received Date: 3 December 2013
Available Online: 16 January 2014
Fund Project: This work is financially supported by the National Instrumentation Program (No. 2011YQ17006702) (No. 2011YQ17006702)

The imaging mass spectrometry (IMS) technology has experienced a rapid development in recent years. A new IMS technology which is based on air flowassisted ionization (AFAI) was reported. It allows for the convenient pretreatment of the samples and can image a large area of sample in a single measurement with high sensitivity. The AFAI in DESI mode was used as the ion source in this paper. The new IMS method is named AFADESI-IMS. The adoption of assisted air flow makes the sample pretreatment easy and convenient. An optimization of the distance between the ion transport tube and MS orifice increases the sensitivity of the system. For data processing, a program based on MATLAB with the function of numerical analysis was developed. A theoretical imaging resolution of a few hundred microns can be achieved. The composite AFAI-IMS images of different target analytes were imaged with high sensitivity. A typical AFAI-IMS image of the whole-body section of a rat was obtained in a single analytical measurement. The ability to image a large area for relevant samples in a single measurement with high sensitivity and repeatability is a significant advantage. The method has enormous potentials in the MS imaging of large and complicated samples.
- Imaging mass spectrometry,
- Air flow assisted ionization imaging mass,
- spectrometry,
- Whole-body imaging,
- Large and complicated sample imaging
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