Citation: Cheng Cheng Liu, Yun Liu, Hui Xiang Wang, Yan Bo Qi, Peng Yuan Yang, Bao Hong Liu. Apolipoprotein B100 analysis in microchip with electrochemical detection[J]. Chinese Chemical Letters, ;2011, 22(11): 1343-1346. doi: 10.1016/j.cclet.2011.05.028 shu

Apolipoprotein B100 analysis in microchip with electrochemical detection

Department of Chemistry and Institute of Biomedical Science, Fudan University, Shanghai 200433, China

Received Date: 11 March 2011

Fund Project: This work is supported by 973 Program (No.2007CB714506), NSFC (Nos.20925517, 21005019), STCSM (Nos.10XD1406000, 09JC1402600) and Shanghai Leading Academic Discipline Project (No.B109).

Apolipoprotein B100 (apoB-100) is a major protein of the cholesterol-rich low-density lipoprotein (LDL) and reflects a better assessment of total atherogenic burden to the vascular system than LDL. In this work, a simple and sensitive method has been developed to determine picoliter apoB-100s using the PMMA microfluidic chip coupled with electrochemical detection system. This method performs very well with a detectable linear range of 1-800 pg/mL and a detection limit of 1 pg/mL. A real serum sample has further been detected by this microchip-based biosensor. The results show that this kind of method is practicable and has the potential application in clinical analysis and diagnosis.
- Microchip,
- ApoB-100,
- Immunoassay,
- Electrochemical detection
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