Citation: Zi Cheng Li, Lang Zhu Chi, Jin Kun Zhu, Yin Yan Zhang, Qing Jiang Wang, Pin Gang He, Yu Zhi Fang. Simultaneous determination of active ingredients in blueberry wine by CE-AD[J]. Chinese Chemical Letters, ;2011, 22(10): 1237-1240. doi: 10.1016/j.cclet.2011.04.011 shu

Simultaneous determination of active ingredients in blueberry wine by CE-AD

Department of Chemistry, East China Normal University, Shanghai 200062, China

Received Date: 10 January 2011

Fund Project: This work is financially supported by Natural Science Research Foundation from Shanghai Science and Technology Committee (No.08142201400).

A method based on capillary electrophoresis coupled with amperometric detection (CE-AD) has been developed for the separation and determination of kaempferol, ferulic acid, vanillic acid, caffeic acid, gallic acid and protocatechuic acid in blueberry wine for the first time. In order to get the optimum conditions of separation, the effects of working electrode potential, pH value and concentration of running buffer, separation voltage and injection time on CE-AD were investigated. Under the optimum conditions, the analytes could be separated in Na₂B₄O₇-KH₂PO₄ buffer at pH 7.8 within 18 min. A 300 μm diameter carbon disk electrode had good current responses at +0.95 V (vs. SCE) for all analytes. The responses were linear with concentrations over three orders of magnitude with detection limits (S/N=3) at 10^-8 g/mL magnitude for the analytes and the recoveries were in the range of 95.8-106.7%. The method could be successfully applied to the analysis of real sample with satisfactory results and therefore recommended for use by the quality control departments of fruit wine producers.
- Application,
- Capillary electrophoresis,
- Amperometric detection Blueberry wine
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