Citation: Chang Feng Li, Li Ming Du, Hao Wu, Ying Xia Chang. Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method[J]. Chinese Chemical Letters, ;2011, 22(7): 851-854. doi: 10.1016/j.cclet.2010.12.029 shu

Determination of L-phenylalanine by cucurbit[7]uril sensitized fluorescence quenching method

a Analytical and Testing Center, Shanxi Normal University, Linfen 041004, China;
b School of Chemistry and Materials Science, Shanxi Normal University, Linfen 041004, China

Received Date: 14 October 2010

Fund Project: This work was supported by the Research Fund for the Doctoral Program of Higher Education of China (No.20091404110001) and the foundation of Shanxi Normal University (No.ZR1007).

The determination method of L-phenylalanine (LPA) by fluorescence quenching was developed. The assay was based on the combination of the cucurbit[7]uril (CB[7]) with palmatine hydrochloride (PAL) reaction. In the presence of CB[7], the fluorescence of PAL was quenched by LPA which can be employed to detect LPA. Under the optimal conditions, a linear range 3.63×10^-8-9.68×10^-6 mol/L and a detection limit 1.27×10^-8 mol/L of LPA were obtained. The relative standard deviation (R.S.D) was 1.8% obtained from a series of11 standards each containing 6.05×10^-6 mol/L oLPA. This paper also discusses the mechanism of fluorescence indicator probe.
- L-phenylalanine,
- Palmatine hydrochloride,
- Cucurbit[7]uril,
- Fluorescent probe
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