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Citation: Ru Feng, Jian Gong Shi, Xiao Wei Liu, Chun Tao Che, John H. K. Yeung, Yan Wang. Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry[J]. Chinese Chemical Letters, ;2011, 22(7): 839-842. doi: 10.1016/j.cclet.2010.12.021 shu

Identification of the metabolites of biologically active xanthones isolated from Halenia elliptica D.Don by high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry

a Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China;
b Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, China;
c School of Chinese Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China;
d Department of Pharmacology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, NT, Hong Kong SAR, China

Received Date: 11 October 2010

Fund Project: We gratefully acknowledge the supports of the National Natural Science Foundation of China (No.30873115) and National Science and Technology Special Projects (Nos.2009zx09301-003-5-1 and 2009zx09301-003-7-1).This study was also supported by the analytical center of the Peking branch of Japanese Shimadzu Corporation to provide the LCMSn-IT-TOF system.

Metabolism study has been carried out on 1-hydroxy-2,3,5-trimethoxyxanthone (HM-1) and 1-hydroxy-2,3,4,7-tetramethoxyxanthone (HM-2), which are two biologically active ingredients isolated from the Tibetan herb, <Halenia elliptica D.Don., in rat liver microsomes in vitro. A method of high performance liquid chromatography coupled to ion trap time-of-flight mass spectrometry (LCMSn-ESI-IT-TOF) was applied to analyze metabolites of HM-1 and HM-2 on line, and five metabolites were identified containing 1,5-dihydroxy-2,3-dimethoxyxanthone (HM-5), 1,7-dihydroxy-2,3,4-trimethoxyxanthone (HM-9), 1,4, 7- trihydroxy-2,3-dimethoxyxanthone (HM-10), 1,4-dihydroxy-2,3,7-trimethoxyxanthone (HM-11) and 1,2-dihydroxy-3,4,7- trimethoxyxanthone (HM-12). Among these metabolites, HM-9, HM-11, and HM-12 were isomers mutually. The results indicated that HM-1 and HM-2 occurred Phase I metabolic reaction of demethylation in rat microsomes in vitro.
- Halenia elliptica,
- Xanthone,
- Biotransformation,
- LCMSⁿ-ESI-IT-TOF,
- Metabolite identification
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