Citation: Fang Fang, Xue Jun Kang, Zhi Yong Liu, Yu Qin Ma, Zhong Ze Gu. Use of packed-fiber solid-phase extraction for sample clean-up and preconcentration of vitamin B 12 before determination[J]. Chinese Chemical Letters, ;2009, 20(12): 1491-1494. doi: 10.1016/j.cclet.2009.06.030
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A rapid and simple preconcentration step applying packed-fiber solid-phase extraction columns has been investigated to vitamin B 12.The extraction performance of the new method was investigated preliminarily on vitamin functional drink.The analysis used a reversed-phase C18 column, with a photo-diode array detector at 220 nm.The samples were preconcentrated with packed-fiber solid-phase extraction columns.Good linearity was observed in vitamin functional drink.The repeatability of extraction performance, expressed as relative standard deviations, was from 3.5% to 4.3%.The limit of detection (LOD) is 5 ng mL-1(S/N=3).Finally, the method had been applied for the determination of vitamin B 12 in vitamin functional drink.
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