Citation: Feng Qu, Hao Qin, Min Dong, Dong Xu Zhao, Xin Ying Zhao, Jing Hua Zhang. Selective separation and enrichment of proteins in aqueous two-phase extraction system[J]. Chinese Chemical Letters, ;2009, 20(9): 1100-1102. doi: 10.1016/j.cclet.2009.04.023 shu

Selective separation and enrichment of proteins in aqueous two-phase extraction system

a School of Life Science, Beijing Institute of Technology, Beijing 100081, China;
b Beijing Center for Physical and Chemical Analysis, Beijing 100089, China

Received Date: 19 December 2008

Fund Project: National Natural Science Foundation of China (No.20875009).This work is supported by the National Basic Research Program of China (973 program No.2007CB914101)

A simple aqueous two-phase extraction system (ATPS) of PEG/phosphate was proposed for selective separation and enrichment of proteins. The combination of ATPE with HPLC was applied to identify the partition of proteins in two phases. Five proteins (bovine serum albumin, Cytochrome C, lysozyme, myoglobin, and trypsin) were used as model proteins to study the effect of phosphate concentration and pH on proteins partition. The PEG/phosphate system was firstly applied to real human saliva and plasma samples, some proteins showed obviously different partition in two phases. The primary results manifest the selective separation and enrichment of proteins in ATPS provided the potential for high abundance proteins depletion in proteomics.
- Aqueous two-phase extraction,
- Saliva and plasma,
- Proteins,
- Selective separation and enrichment
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