Citation: Fan Liu, Jian Bin Zhang, Zheng Fu Zhang, Xiao Yan Zhang, Zuo Peng Du, Xiong Hui Wei. Chelerythrine chloride binding of guanosine in aqueous solution[J]. Chinese Chemical Letters, ;2009, 20(1): 66-70. doi: 10.1016/j.cclet.2008.10.023 shu

Chelerythrine chloride binding of guanosine in aqueous solution

a College of Chemical Engineering, Inner Mongolia University of Technology, Huhhot 010051, China;
b Department of Applied Chemistry, College of Chemistry & Molecular Engineering, Peking University, Beijing 100190, China

Received Date: 28 August 2008

Fund Project: This project is financed by Jiangxi Boyuan Industry Co., Ltd. (Jiangxi province, China). Thanks to Professor Wenting Hua and Professor Hongcheng Gao (Peking University, China) for their suggestions on the interaction mechanism of CHE with guanosine.

In this work, the interaction between chelerythrine (CHE) and guanosine is studied usingUV-vis and fluorescence measurements at various temperatures. The UV-vis spectra show that the increasing guanosine concentrations result in the decreasing absorption intensity and red shift of CHE E absorption band (267 nm). The fluorescence spectra are fitted to linear analysis, yielding a binding constant of 1.04×10⁴ L/mol at 298.15 K of CHE with guanosine. Besides, with △_rH_m^Θ=-8.26 kJ=mol, △_rG_m^Θ=-22.90 kJ=mol,and △_rS_m^Θ=-49.38 J/(molK)the interaction should be entropy-driven and exterothermic.
- Chelerythrine(CHE),
- Guanosine,
- UV-vis spectra,
- Fluorescence spectra
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