Citation: Ming Qin Chen, Zhi Yong Lin, Hao Qian. Preparation of thiophilic paramagnetic adsorbent for separation of antibodies[J]. Chinese Chemical Letters, ;2008, 19(12): 1495-1498. doi: 10.1016/j.cclet.2008.09.050 shu

Preparation of thiophilic paramagnetic adsorbent for separation of antibodies

College of Material Science and Engineering, Huaqiao University, Quanzhou 362021, China

Received Date: 16 June 2008

Fund Project: We are grateful to the National Natural Science Foundation of China (No. 50603007 and 50673030) for financial support of this work.

The micron-sized mierospheres with superparamagnetic property were synthesized with vinyl acetate and divinylbenzene by microsuspension polymerization. After the complete alcoholysis, these hydroxyl-functionalized microspheres were activated by divinylfone and modified with mercaptoethanol to prepare the thiophilic magnetic adsorbent, which was used to specifically isolate immunoglobulin G (IgG) from human serum. This thiophilic magnetic adsorbent performed an evident salt-dependent adsorption behavior for IgG. Due to their salt-promoted adsorption towards IgG under high salt concentration, the absorbed antibodies could be extracted in low salt concentration with high purity.
- Superparamagnetic,
- Thiophilic,
- Microsuspension,
- Immunoglobulin G,
- Isolation
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