Citation: Jiang Jiang, Qiong Jie Wu, Xiang Yang Cai, Long Gen Zhu, Wen Ji Wang. Mass spectrometry assisted assignments of binding and cleavage sites of Zn(Ⅱ) complex towards oxidized insulin B chain[J]. Chinese Chemical Letters, ;2009, 20(1): 71-75. doi: 10.1016/j.cclet.2008.09.043 shu

Mass spectrometry assisted assignments of binding and cleavage sites of Zn(Ⅱ) complex towards oxidized insulin B chain

a College of Chemistry and Chemical Engineering, Fuzhou University, Fuzhou 350002, China;
b College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
c State Key Laboratory of Coordination Chemistry, Nanjing University, Nanjing 210093, China

Received Date: 11 June 2008

The electrospray ionization mass spectrometry investigation showed that the binding sites of [ZnL]²⁺, where L is 2-[bis(2-aminoethyl)amino]ethanol, with oxidized insulin B chain are Phe1, His5 and Arg22, which lead to the selective cleavages of the peptide bonds at Phe1-Val2, His5-Leu6, Glu21-Arg22, and Arg22-Gly23 of oxidized insulin B chain.
- Oxidized insulin B chain,
- Zn(Ⅱ)complex,
- Mass spectrometry,
- Hydrolytic cleavage
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