Citation: Jiang Hua Zhong, Peng Qin, Wei Sun, Kui Jiao. Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels[J]. Chinese Chemical Letters, ;2008, 19(10): 1244-1247. doi: 10.1016/j.cclet.2008.06.036 shu

Simultaneous electrochemical DNA hybridization assay for PAT and FMV 35S gene sequence using quantum dots as labels

College of Chemistry and Molecular Engineering, Key Laboratory of Eco-Chemical Engineering of Ministry ofEducation, Qingdao University of Science and Technology, Qingdao 266042, China

Received Date: 26 March 2008

Fund Project: We are grateful to the financial support of the National Natural Science Foundation of China (Nos. 20405008, 20635020).

An electrochemical method for the simultaneous detection of two different DNA sequenees from PAT and FMV 35S gene sequence using CdS and PbS quantum dots (QDs)as labels was described.The QDs were readily functionalized with oligonucleotides as electrochemical DNA probes and selectively hybridized to the complementary sequences immobilized on the microplate.The QDs anchored on the hybrids were dissolved in the solution by the oxidation of HNO₃ and further detected by a sensitive differential pulse anodic stripping voltammetric method(DPASV).The DPASV signals of the oxidation of Cd²⁺ and Pb²⁺ ions present in the solution were difierent and reflected the identity of corresponding ssDNA targets sequences.
- Electrochemical DNA assay,
- Quantum dots,
- Differential pulse anodic stripping voltammetry,
- Mieroplate,
- Simultaneous detection
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