Citation: Hai Yan Wang, Chun Gui Zhao, Ye Hong Zhou, Shao Min Shuang, Chuan Dong. Interference study on the free calcium in the human peripheral lymphocyte by acrylamide[J]. Chinese Chemical Letters, ;2008, 19(6): 756-757. doi: 10.1016/j.cclet.2007.12.023 shu

Interference study on the free calcium in the human peripheral lymphocyte by acrylamide

a School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
b Medical College of Shanxi Datong University, Datong 037000, China

Received Date: 9 October 2007

The role of acrylamide on the human peripheral lymphocytes was studied by laser scanning confocal microscopy technique and fluo-3. The calibration value of the apparent dissociation constant (K_d) of the fluo-3-Ca²⁺ complex was obtained as 4.83×10^-7mol/L. Acrylamide ( < 54μg/mL) evoked a rise in free intracellular calcium concentration [Ca²⁺]_i, in a dose dependent manner. Acrylamide induced the increase of [Ca²⁺]_i was discussed in detail.
- Laser scanning confocal microscopy,
- Acrylamide,
- Lymphocytes,
- Fluo-3,
- [Ca²⁺]_i
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