Citation: Rong Min Yu, Liang Bin Zhou, Chun Yan Yan, Guo Yan Duan, Yu Zhao. Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum[J]. Chinese Chemical Letters, ;2008, 19(1): 76-78. doi: 10.1016/j.cclet.2007.10.047 shu

Two new coumarin glucosides biosynthesized by transgenic hairy roots of Polygonum multiflorum

a College of Pharmacy, Jinan University, Guangzhou 510632, China;
b Department of Traditional Chinese Medicines and Natural Drug Research, College of Pharmaceutical Sciences, Zhejiang University, 310031 Hangzhou, China

Received Date: 27 August 2007

Fund Project: This research was financially supported by Natural Sciences Foundation of Guangdong(No.04010461).We thank Dr.Dongbo Yu of University of Texas Southwestern Medical Center, USA, for his check in our manuscript.

The glycosylation of hydroxylcoumarin was investigated by using suspension cultures of hairy roots of Polygonum multiflorum, Two new coumarin glucosides (3 and 4) were biosysthesized by regioselectively glycosylation of corresponding substrates (1 and 2) in the system. The structures of two products were identified as 7-hydroxy-4-methylcoumarin 5-O-β-D-glucopyranoside and 7-hydroxy-3,4-dimethylcoumarin 5-O-β-D-glucopyranoside on the ground of chemical and spectroscopic methods, respectively.
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