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Citation:
Su Yuan Zhou, Xiao Yan Wei. Determination of vinyl orientation of mouse neuroglobin by 2D nuclear Overhauser spectroscopy[J]. Chinese Chemical Letters,
;2007, 18(3): 319-321.
doi:
10.1016/j.cclet.2007.01.033
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1H NMR has been used to determine the 2-, 4-vinyl orientation of heme active site from oxidized mouse neuroglobin (mNgb).The NOEs between 3-methyl and Hα, Hβ of 2-vinyl, together with the NOEs between 5-methyl and Hα, Hβ of 4-vinyl, allowed the unambiguous determination of trans and cis orientations for the 2-and 4-vinyl groups in the mNgb, respectively.
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Keywords:
- Heme,
- Vinyl orientation,
- Neuroglobin
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