Citation: Dan Dan Wang, Hai Yan Wang, Ye Hong Zhou, Chun Gui Zhao, Chuan Dong, Shao Min Shuang. Study on the effects of cefotaxime on intracellular Ca²⁺ in human peripheral lymphocytes by fluoremetry[J]. Chinese Chemical Letters, ;2007, 18(4): 424-426. doi: 10.1016/j.cclet.2006.12.044 shu

Study on the effects of cefotaxime on intracellular Ca²⁺ in human peripheral lymphocytes by fluoremetry

a School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China;
b Medical College of Shanxi Datong University, Datong 037000, China

Received Date: 30 October 2006

Fund Project: This work is supported by the National Natural Science Foundation of China (No. 20575038). All the authors express their deep thanks.

Characteristic of Fura-2-Ca²⁺ interaction was studied based on the fluorescence technique. The apparent dissociation constants (K_d) of the Fura-2-Ca²⁺ complex were determined at different temperature. The effect of cefotaxime (CEFA) on intracellular Ca²⁺ concentration ([Ca²⁺]_i) was discussed by using a ratiometric fluorescence dye Fura-2 as a probe. The basal [Ca²⁺]_i in resting human peripheral lymphocytes was 100±7 nmol/L but after treatment with cefotaxime, the changes of [Ca²⁺]_i were observed in different conditions. In the concentration range of 1-30 μmol/L of cefotaxime [Ca²⁺]_i increased, as a result of releasing intracellular Ca²⁺ stores. Higher concentration of cefotaxime (50-500 μmol/L) stimulated to decrease of [Ca²⁺]_i.
- Fura-2,
- Lymphocytes,
- Cefotaxime,
- [Ca²⁺]_i
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